Isothermal titration calorimetry to determine association constants for high-affinity ligands

Nature Protocols

Volumn 1, Issue 1, 2006, Pages 186-191

  Velazquez Campoy, Adrian ^a   Freire, Ernesto ^b  

^a UNIVERSITY OF ZARAGOZA   (Spain)

^b JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

LIGAND; PROTEIN;

ARTICLE; CALORIMETRY; CHEMISTRY; DRUG DESIGN; METABOLISM; METHODOLOGY; THERMODYNAMICS; TITRIMETRY;

CALORIMETRY; DRUG DESIGN; LIGANDS; PROTEINS; THERMODYNAMICS; TITRIMETRY;

EID: 34250641961     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2006.28     Document Type: Article

References (9)

1. Sigurskjold, B.W. Exact analysis of competition ligand binding by displacement isothermal titration calorimetry. Anal. Biochem. 277, 260-266 (2000).
2. Velazquez-Campoy, A. & Freire E. ITC in the post-genomic era...? Priceless. Biophys. Chem. 115, 115-124 (2005).
3. Velazquez-Campoy, A. & Freire, E. Incorporating target heterogeneity in drug design. J. Cell. Biochem. S37, 82-88 (2001).
4. Velazquez-Campoy, A., Kiso, Y. & Freire, E. The binding energetics of first- and second-generation HIV-1 protease inhibitors: Implications for drug design. Arch. Biochem. Biophys. 390, 169-175 (2001).
5. Ohtaka, H., Velazquez-Campoy, A., Xie, D. & Freire, E. Overcoming drug resistance in HIV-1 chemotherapy: The binding thermodynamics of amprenavir and TMC-126 to wild-type and drug-resistant mutants of the HIV-1 protease. Protein Sci. 11, 1908-1916 (2002).
6. Velazquez-Campoy, A., Vega, S. & Freire, E. Amplification of the effects of drug resistance mutations by background polymorphisms in HIV-1 protease from African subtypes. Biochemistry 41, 8613-8619 (2002).
7. Bradshaw, J.M., Mitaxov, V. & Waksman, G. Investigation of phosphotyrosine recognition by the SH2 domain of the Src kinase. J. Mol. Biol. 293, 971-985 (1999).
8. Zhang, Y.-L. & Zhang Z.-Y. Low-affinity binding determined by titration calorimetry using a high-affinity coupling ligand: A thermodynamic study of ligand binding to tyrosine phosphatase 1B. Anal. Biochem. 261, 139-148 (1998).
9. Todd, M.J., Semo, N. & Freire, E. The structural stability of the HIV-1 protease. J. Mol. Biol. 283, 475-488 (1998).