Effects of HAb18G/CD147 Knockout on Hepatocellular Carcinoma Cells In Vitro Using a Novel Zinc-Finger Nuclease-Targeted Gene Knockout Approach

Cell Biochemistry and Biophysics

Volumn 71, Issue 2, 2015, Pages 881-890

  Li, Hong Wei ^a   Yang, Xiang Min ^a   Tang, Juan ^a   Wang, Shi Jie ^a   Chen, Zhi Nan ^a   Jiang, Jian Li ^a  

^a FOURTH MILITARY MEDICAL UNIVERSITY   (China)

Author keywords

HAb18G CD147; Knockout; p53; SMMC 7721; ZFNs

Indexed keywords

BSG PROTEIN, HUMAN; CD147 ANTIGEN; PROTEIN P53; RIBONUCLEASE; ZINC FINGER PROTEIN;

APOPTOSIS; CHEMISTRY; GENE INACTIVATION; GENETICS; HUMAN; LIVER CELL CARCINOMA; LIVER TUMOR; METABOLISM; PROCEDURES; SIGNAL TRANSDUCTION; TUMOR CELL LINE;

ANTIGENS, CD147; APOPTOSIS; CARCINOMA, HEPATOCELLULAR; CELL LINE, TUMOR; ENDORIBONUCLEASES; GENE KNOCKOUT TECHNIQUES; HUMANS; LIVER NEOPLASMS; SIGNAL TRANSDUCTION; TUMOR SUPPRESSOR PROTEIN P53; ZINC FINGERS;

EID: 84925502945     PISSN: 10859195     EISSN: 15590283     Source Type: Journal    
DOI: 10.1007/s12013-014-0278-9     Document Type: Article

References (19)

1. Jemal, A., Bray, F., Center, M. M., Ferlay, J., Ward, E., et al. (2011). Global cancer statistics. CA: A Cancer Journal for Clinicians,61, 69–90.
2. But, D. Y., Lai, C. L., & Yuen, M. F. (2008). Natural history of hepatitis-related hepatocellular carcinoma. World Journal of Gastroenterology,14, 1652–1656.
3. Xu, J., Xu, H. Y., Zhang, Q., Song, F., Jiang, J. L., et al. (2007). HAb18G/CD147 functions in invasion and metastasis of hepatocellular carcinoma. Molecular Cancer Research,5, 605–614.
4. Li, Y., Xu, J., Chen, L., Zhong, W. D., Zhang, Z., et al. (2009). HAb18G (CD147), a cancer-associated biomarker and its role in cancer detection. Histopathology,54, 677–687.
5. Ke, X., Li, L., Dong, H. L., & Chen, Z. N. (2012). Acquisition of anoikis resistance through CD147 upregulation: A new mechanism underlying metastasis of hepatocellular carcinoma cells. Oncology Letters,3, 1249–1254.
6. Gou, X., Ru, Q., Zhang, H., Chen, Y., Li, L., et al. (2009). HAb18G/CD147 inhibits starvation-induced autophagy in human hepatoma cell SMMC7721 with an involvement of Beclin 1 down-regulation. Cancer Science,100, 837–843.
7. Flatmark, K., Borgen, E., Nesland, J. M., Rasmussen, H., Johannessen, H. O., et al. (2011). Disseminated tumour cells as a prognostic biomarker in colorectal cancer. British Journal of Cancer,104, 1434–1439.
8. Hu, J., Dang, N., Yao, H., Li, Y., Zhang, H., et al. (2010). Involvement of HAb18G/CD147 in T cell activation and immunological synapse formation. Journal of Cellular and Molecular Medicine,14, 2132–2143.
9. Vandesompele, J., De Preter, K., Pattyn, F., Poppe, B., Van Roy, N., et al. (2002). Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biology,3, H34.
10. Geske, F. J., Lieberman, R., Strange, R., & Gerschenson, L. E. (2001). Early stages of p53-induced apoptosis are reversible. Cell Death and Differentiation,8, 182–191.
11. Tang, Y., Nakada, M. T., Rafferty, P., Laraio, J., McCabe, F. L., et al. (2006). Regulation of vascular endothelial growth factor expression by EMMPRIN via the PI3K-Akt signaling pathway. Molecular Cancer Research,4, 371–377.
12. Keith, B., Johnson, R. S., & Simon, M. C. (2012). HIF1alpha and HIF2alpha: Sibling rivalry in hypoxic tumour growth and progression. Nature Reviews Cancer,12, 9–22.
13. Urlaub, G., & Chasin, L. A. (1980). Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity. Proceedings of the National Academy of Sciences of the United States of America,77, 4216–4220.
14. Mansour, S. L., Thomas, K. R., & Capecchi, M. R. (1988). Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes. Nature,336, 348–352.
15. Suzuki, S., Sato, M., Senoo, H., & Ishikawa, K. (2004). Direct cell–cell interaction enhances pro-MMP-2 production and activation in co-culture of laryngeal cancer cells and fibroblasts: Involvement of EMMPRIN and MT1-MMP. Experimental Cell Research,293, 259–266.
16. Liu, Y. (1991). [Localization of hepatocellular carcinoma with monoclonal antibodies]. Zhonghua Yi Xue Za Zhi,71, 362–365. 26.
17. Zhao, P., Zhang, W., Wang, S. J., Yu, X. L., Tang, J., et al. (2011). HAb18G/CD147 promotes cell motility by regulating annexin II-activated RhoA and Rac1 signaling pathways in hepatocellular carcinoma cells. Hepatology,54, 2012–2024.
18. Lehmann, B. D., & Pietenpol, J. A. (2012). Targeting mutant p53 in human tumors. Journal of Clinical Oncology,30, 3648–3650.
19. Shi, D., & Gu, W. (2012). Dual roles of MDM2 in the regulation of p53: Ubiquitination dependent and ubiquitination independent mechanisms of MDM2 repression of p53 activity. Genes Cancer,3, 240–248.