mRNA secondary structure engineering of Thermobifida fusca endoglucanase (Cel6A) for enhanced expression in Escherichia coli

World Journal of Microbiology and Biotechnology

Volumn 31, Issue 3, 2015, Pages 499-506

  Ali, Imran ^a   Asghar, Rehana ^a   Ahmed, Sajjad ^b   Sajjad, Muhammad ^b   Tariq, Muhammad ^a   Waheed Akhtar, M ^b  

^a MIRPUR UNIVERSITY OF SCIENCE AND TECHNOLOGY MUST   (Pakistan)

^b UNIVERSITY OF THE PUNJAB   (Pakistan)

Author keywords

Endoglucanase; Enhanced expression; Escherichia coli; Residual activity; Synonymous mutations; Thermobifida fusca

Indexed keywords

ESCHERICHIA COLI; METAL IONS; METALS; RECOMBINANT PROTEINS; THERMODYNAMICS;

ENDOGLUCANASES; ENHANCED EXPRESSION; RESIDUAL ACTIVITY; SYNONYMOUS MUTATIONS; THERMOBIFIDA FUSCA;

ENZYME ACTIVITY;

BAGASSE; CELLULASE; CELLULOSE; ENZYME ACTIVATOR; ENZYME INHIBITOR; MESSENGER RNA; METAL; RECOMBINANT PROTEIN; RIBOSOME RNA;

ACTINOBACTERIA; BASE PAIRING; BINDING SITE; BIOSYNTHESIS; CHEMICAL STRUCTURE; CHEMISTRY; CONFORMATION; ENTEROBACTERIA PHAGE T7; ENZYME STABILITY; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; HYDROLYSIS; ISOLATION AND PURIFICATION; METABOLIC ENGINEERING; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PH; POINT MUTATION; PROCEDURES; PROMOTER REGION; TEMPERATURE;

ACTINOBACTERIA; BACTERIOPHAGE T7; BASE PAIRING; BASE SEQUENCE; BINDING SITES; CELLULASE; CELLULOSE; ENZYME ACTIVATORS; ENZYME INHIBITORS; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; METABOLIC ENGINEERING; METALS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; POINT MUTATION; PROMOTER REGIONS, GENETIC; RECOMBINANT PROTEINS; RNA, MESSENGER; RNA, RIBOSOMAL; TEMPERATURE;

EID: 84925494418     PISSN: 09593993     EISSN: 15730972     Source Type: Journal    
DOI: 10.1007/s11274-015-1806-5     Document Type: Article

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