Single-experiment displacement assay for quantifying high-affinity binding by isothermal titration calorimetry

Methods

Volumn 76, Issue , 2015, Pages 116-123

  Krainer, Georg ^a,b   Keller, Sandro ^a  

^a University of Koblenz Landau   (Germany)

^b DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

Competition assay; Metal ion chelation; Protein inhibitor interactions; Protein ligand interactions

Indexed keywords

AMPRENAVIR; LIGAND; METAL ION; NELFINAVIR; CALCIUM CHLORIDE; EDETIC ACID; EGTAZIC ACID; ENZYME INHIBITOR; N-(HYDROXYETHYL)ETHYLENEDIAMINETRIACETIC ACID; PROTEIN; PROTEIN BINDING;

ARTICLE; ASSAY; BINDING SITE; CHELATION; ENTHALPY; ISOTHERMAL TITRATION CALORIMETRY; LIMIT OF QUANTITATION; PRIORITY JOURNAL; SINGLE EXPERIMENT DISPLACEMENT ASSAY; STOICHIOMETRY; THERMODYNAMICS; THERMOGRAPHY; ANALOGS AND DERIVATIVES; CALORIMETRY; CHEMISTRY; COMPUTER SIMULATION; METABOLISM; PROCEDURES;

CALCIUM CHLORIDE; CALORIMETRY; COMPUTER SIMULATION; EDETIC ACID; EGTAZIC ACID; ENZYME INHIBITORS; LIGANDS; PROTEIN BINDING; PROTEINS; THERMODYNAMICS;

EID: 84925449320     PISSN: 10462023     EISSN: 10959130     Source Type: Journal    
DOI: 10.1016/j.ymeth.2014.10.034     Document Type: Article

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