Expression levels of chaperones influence biotransformation activity of recombinant Escherichia coli expressing Micrococcus luteus alcohol dehydrogenase and Pseudomonas putida Baeyer-Villiger monooxygenase

Biotechnology and Bioengineering

Volumn 112, Issue 5, 2015, Pages 889-895

  Baek, A Hyong ^a   Jeon, Eun Yeong ^a   Lee, Sun Mee ^b   Park, Jin Byung ^a  

^a Ewha Womans University   (South Korea)

^b Kyungil University   (South Korea)

Author keywords

Alcohol dehydrogenase; Baeyer Villiger monooxygenase; Chaperones; Escherichia coli; Ricinoleic acid; Thermosome; Whole cell biocatalysis; prefoldin

Indexed keywords

BACTERIA; BIOCATALYSTS; BIOCONVERSION; CELLS; CYTOLOGY; ENZYMES; ESCHERICHIA COLI;

ALCOHOL DEHYDROGENASE; BAEYER-VILLIGER MONOOXYGENASES; CHAPERONES; RICINOLEIC ACID; THERMOSOME; WHOLE-CELL BIOCATALYSIS;

ENZYME ACTIVITY;

ALCOHOL DEHYDROGENASE; CHAPERONE; RICINOLEIC ACID; THERMOSOME; UNSPECIFIC MONOOXYGENASE; MIXED FUNCTION OXIDASE; PREFOLDIN; RECOMBINANT PROTEIN;

ARTICLE; BAEYER VILLIGER REACTION; BIOCATALYSIS; BIOCATALYST; BIOTRANSFORMATION; CARBON SOURCE; ENZYME ACTIVITY; ESCHERICHIA COLI; METHANOCALDOCOCCUS JANNASCHII; MICROCOCCUS LUTEUS; NONHUMAN; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN STRUCTURE; PSEUDOMONAS PUTIDA; WESTERN BLOTTING; WHOLE CELL; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; METHANOCALDOCOCCUS;

ARCHAEA; ESCHERICHIA COLI; METHANOCALDOCOCCUS JANNASCHII; MICROCOCCUS LUTEUS; PSEUDOMONAS PUTIDA;

ALCOHOL DEHYDROGENASE; BIOTRANSFORMATION; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; METHANOCALDOCOCCUS; MICROCOCCUS LUTEUS; MIXED FUNCTION OXYGENASES; MOLECULAR CHAPERONES; PSEUDOMONAS PUTIDA; RECOMBINANT PROTEINS; RICINOLEIC ACIDS;

EID: 84925428381     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25521     Document Type: Article

References (26)

1. Bergeron LM, Gomez L, Whitehead TA, Clark DS. 2009a. Self-renaturing enzymes: design of an enzyme-chaperone chimera as a new approach to enzyme stabilization. Biotechnol Bioeng 102:(5):1316-1322.
2. Bergeron LM, Lee C, Tokatlian T, Hollrigl V, Clark DS. 2008. Chaperone function in organic co-solvents: Experimental characterization and modeling of a hyperthermophilic chaperone subunit from Methanocaldococcus jannaschii. Biochim Biophys Acta-Prot Proteom 1784:(2):368-378.
3. Bergeron LM, Tokatlian T, Gomez L, Clark DS. 2009b. Redirecting the inactivation pathway of penicillin amidase and increasing amoxicillin production via a thermophilic molecular chaperone. Biotechnol Bioeng 102:(2):417-424.
4. Doyle SM, Wickner S. 2009. Hsp104 and ClpB: protein disaggregating machines. Trends Biochem Sci 34:(1):40-48.
5. Franceschini S, van Beek HL, Pennetta A, Martinoli C, Fraaije MW, Mattevi A. 2012. Exploring the structural basis of substrate preferences in Baeyer-Villiger monooxygenases. Insight from steroid monooxygenase. J Biol Chem 287:(27):22626-22634.
6. Hoffmann F, Rinas U. 2004. Stress induced by recombinant protein production in Escherichia coli. Adv Biochem Eng Biotechnol 89:73-92.
7. Hirtreiter AM, Calloni G, Forner F, Scheibe B, Puype M, Vandekerckhove J, Mann M, Hartl FU, Hayer-Hartl M. 2009. Differential substrate specificity of group I and group II chaperonins in the archaeon Methanosarcina mazei. Mol Microbiol 74:(5):1152-1168.
8. Jang H-Y, Jeon E-Y, Baek A-H, Lee S-M, Park JB. 2014. Production of ω-hydroxyundec-9-enoic acid and n-heptanoic acid from ricinoleic acid by recombinant Escherichia coli-based biocatalyst. Process Biochem 49:617-622.
9. Jeon E-Y, Bornscheuer UT, Park J-B.; 2014. Enzyme fusion for whole-cell biotransformation of long chain sec-alcohols into esters. Appl Microbiol Biotechnol Submitted. In press. doi: 10.1007/s00253-015-6392-9
10. Kim YE, Hipp MS, Bracher A, Hayer-Hartl M, Hartl FU. 2013. Molecular chaperone functions in protein folding and proteostasis. Ann Rev Biochem 82:323-355.
11. Kolaj O, Spada S, Robin S, Wall J. 2009. Use of folding modulators to improve heterologous protein production in Escherichia coli. Microb Cell Fact 8:1-17.
12. Laksanalamai P, Narayan S, Luo H, Robb F. 2009. Chaperone action of a versatile small heat shock protein from Methanococcoides burtonii, a cold adapted archaeon. Proteins 75:275-281.
13. Lee S-M, Yun J-Y, Woo J-M, Kang S-H, Yang K-M, Park J-B. 2011. Improving the catalytic activity of cyclohexanone monooxygenase-based whole-cell biocatalysts under substrate toxic conditions. J Kor Soc Appl Biol Chem 54:(6):986-992.
14. Lee W-H, Park J-B, Park K, Kim M-D, Seo J-H. 2007. Enhanced production of e{open}-caprolactone by overexpression of NADPH-regenerating glucose 6-phosphate dehydrogenase in recombinant Escherichia coli harboring cyclohexanone monooxygenase gene. Appl Microbiol Biotechnol 76:329-338.
15. Lim JH, Seo SW, Kim SY, Jung GY. 2013. Model-driven rebalancing of the intracellular redox state for optimization of a heterologous n-butanol pathway in Escherichia coli. Met Eng 20:49-55.
16. Orru R, Dudek HM, Martinoli C, Pazmino DET, Royant A, Weik M, Fraaije MW, Mattevi A. 2011. Snapshots of enzymatic Baeyer-Villiger catalysis. Oxygen activation and intermediate stabilization. J Biol Chem 286:(33):29284-29291.
17. Park YS, Seo SW, Hwang S, Chu HS, Ahn J-H, Kim T-W, Kim D-M, Jung GY. 2007. Design of 5 '-untranslated region variants for tunable expression in Escherichia coli. Biochem Biophys Res Comm 356:(1):136-141.
18. Riesenberg D. 1991. High-cell-density cultivation of Escherichia coli. Curr Opin Biotechnol 2:380-384.
19. Rehdorf J, Kirschner A, Bornscheuer UT. 2007. Cloning, expression and characterization of a Baeyer-Villiger monooxygenase from Pseudomonas putida KT2440. Biotechnol Lett 29:1393-1398.
20. Seo SW, Yang J-S, Cho H-S, Yang J, Kim SC, Park JM, Kim S, Jung GY. 2014. Predictive combinatorial design of mRNA translation initiation regions for systematic optimization of gene expression levels. Sci Rep 4.
21. Seo SW, Yang J-S, Kim I, Yang J, Min BE, Kim S, Jung GY. 2013. Predictive design of mRNA translation initiation region to control prokaryotic translation efficiency. Met Eng 15:67-74.
22. Song JW, Jeon EY, Song DH, Jang HY, Bornscheuer UT, Oh DK, Park JB. 2013. Multistep enzymatic synthesis of long-chain α, ω-dicarboxylic and ω-hydroxycarboxylic acids from renewable fatty acids and plant oils. Ang Chem-Int Ed 52:(9):2534-2537.
23. Song JW, Lee J-H, Bornscheuer UT, Park JB. 2014. Microbial synthesis of medium chain α, ω-dicarboxylic acids and ω-aminocarboxylic acids from renewable long chain fatty acids. Adv Syn Cat 356:1782-1788.
24. Techtmann SM, Robb FT. 2010. Archaeal-like chaperonins in bacteria. PNAS 107:(47):20269-20274.
25. Whitehead TA, Boonyaratanakornkit BB, Hoellrigl V, Clark DS. 2007. A filamentous molecular chaperone of the prefoldin family from the deep-sea hyperthermophile Methanocaldococcus jannaschii. Prot Sci 16:(4):626-634.
26. Yachnin BJ, Sprules T, McEvoy MB, Lau PCK, Berghuis AM. 2012. The substrate-bound crystal structure of a Baeyer-Villiger monooxygenase exhibits a Criegee-like conformation. J Am Chem Soc 134:(18):7788-7795.