Self-renaturing enzymes: Design of an enzyme-chaperone chimera as a new approach to enzyme stabilization

Biotechnology and Bioengineering

Volumn 102, Issue 5, 2009, Pages 1316-1322

  Bergeron, Lisa M ^a   Gomez, Lizabeth ^a   Whitehead, Timothy A ^a   Clark, Douglas S ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Antibiotic synthesis; Chaperone; Fusion protein; Organic solvent; Protein folding

Indexed keywords

AMIDASE; AMOXICILLIN; AQUEOUS-ORGANIC MIXTURES; BIOCATALYSIS; CHAPERONE; CHAPERONIN; CHITIN BINDING DOMAINS; DENATURING CONDITIONS; ENZYME STABILIZATIONS; ENZYME SYSTEMS; FLEXIBLE LINKERS; FUSION PROTEIN; METHANOL MIXTURES; MOLECULAR CHAPERONES; NEW APPROACHES; SPECIFIC ACTIVITIES; TOTAL TURNOVERS;

ANTIBIOTICS; CATALYSTS; CHITIN; ENZYMES; METHANOL; MIXTURES; ORGANIC SOLVENTS; PROTEIN FOLDING;

ENZYME IMMOBILIZATION;

AMOXICILLIN; CHAPERONE; CHAPERONIN; CHITIN; HYBRID PROTEIN; METHANOL; PENICILLIN AMIDASE;

ARTICLE; BINDING SITE; CATALYSIS; CHIMERA; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME STABILITY; IMMOBILIZATION; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN FUNCTION; RENATURATION;

AMOXICILLIN; BIOTECHNOLOGY; ENZYME STABILITY; KINETICS; METHANOL; MODELS, MOLECULAR; MOLECULAR CHAPERONES; PENICILLIN AMIDASE; PROTEIN RENATURATION; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SOLUBILITY; WATER;

EID: 63549119129     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22254     Document Type: Article

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