Genome editing using cas9 nickases

Methods in Enzymology

Volumn 546, Issue C, 2014, Pages 161-174

  Trevino, Alexandro E ^a,b,c,d   Zhang, Feng ^a,b,c,d  

^a BROAD INSTITUTE OF MIT AND HARVARD   (United States)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^c MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^d Massachusetts Institute of Technology Cambridge   (United States)

Author keywords

Cas9; CRISPR; Gene targeting; Genome editing; Nucleases

Indexed keywords

CAS9 ENZYME; ENZYME; UNCLASSIFIED DRUG; DEOXYRIBONUCLEASE I;

ARTICLE; CELL LINE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; DNA END JOINING REPAIR; DNA EXTRACTION; GENE INSERTION; GENOME; INDEL MUTATION; PLASMID; PRIORITY JOURNAL; ANIMAL; CHEMISTRY; CRISPR CAS SYSTEM; ENZYMOLOGY; GENETIC ENGINEERING; GENETICS; HUMAN; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PROCEDURES; STREPTOCOCCUS PYOGENES;

ANIMALIA; EUKARYOTA; MAMMALIA; STREPTOCOCCUS PYOGENES;

ANIMALS; BASE SEQUENCE; CELL LINE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-CAS SYSTEMS; DEOXYRIBONUCLEASE I; GENETIC ENGINEERING; GENOME; HUMANS; MOLECULAR SEQUENCE DATA; STREPTOCOCCUS PYOGENES;

EID: 84925427919     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-801185-0.00008-8     Document Type: Chapter

References (23)

1. R. Barrangou, C. Fremaux, H. Deveau, M. Richards, P. Boyaval, and S. Moineau CRISPR provides acquired resistance against viruses in prokaryotes Science 315 5819 2007 1709 1712 10.1126/science.1138140
2. R. Barrangou, and L.A. Marraffini CRISPR-Cas systems: Prokaryotes upgrade to adaptive immunity Molecular Cell 54 2 2014 234 244 10.1016/j.molcel.2014.03.011
3. J. Boch, and U. Bonas Xanthomonas AvrBs3 family-type III effectors: Discovery and function Annual Review of Phytopathology 48 2010 419 436 10.1146/annurev-phyto-080508-081936
4. K. Chylinski, K.S. Makarova, E. Charpentier, and E.V. Koonin Classification and evolution of type II CRISPR-Cas systems Nucleic Acids Research 42 10 2014 6091 6105 10.1093/nar/gku241
5. L. Cong, F.A. Ran, D. Cox, S. Lin, R. Barretto, and N. Habib Multiplex genome engineering using CRISPR/Cas systems Science 339 6121 2013 819 823 10.1126/science.1231143
6. E. Deltcheva, K. Chylinski, C.M. Sharma, K. Gonzales, Y. Chao, and Z.A. Pirzada CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III Nature 471 7340 2011 602 607 10.1038/nature09886
7. G.L. Dianov, and U. Hubscher Mammalian base excision repair: The forgotten archangel Nucleic Acids Research 41 6 2013 3483 3490 10.1093/nar/gkt076
8. Y. Fu, J.A. Foden, C. Khayter, M.L. Maeder, D. Reyon, and J.K. Joung High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells Nature Biotechnology 31 9 2013 822 826 10.1038/nbt.2623
9. J.E. Garneau, M.E. Dupuis, M. Villion, D.A. Romero, R. Barrangou, and P. Boyaval The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA Nature 468 7320 2010 67 71 10.1038/nature09523
10. G. Gasiunas, T. Sinkunas, and V. Siksnys Molecular mechanisms of CRISPR-mediated microbial immunity Cellular and Molecular Life Sciences 71 3 2014 449 465 10.1007/s00018-013-1438-6
11. P.D. Hsu, E.S. Lander, and F. Zhang Development and applications of CRISPR-Cas9 for genome engineering Cell 157 6 2014 1262 1278 10.1016/j.cell.2014.05.010
12. P.D. Hsu, D.A. Scott, J.A. Weinstein, F.A. Ran, S. Konermann, and V. Agarwala DNA targeting specificity of RNA-guided Cas9 nucleases Nature Biotechnology 31 9 2013 827 832 10.1038/nbt.2647
13. M. Jinek, K. Chylinski, I. Fonfara, M. Hauer, J.A. Doudna, and E. Charpentier A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity Science 337 6096 2012 816 821 10.1126/science.1225829
14. A. Klug The discovery of zinc fingers and their applications in gene regulation and genome manipulation Annual Review of Biochemistry 79 2010 213 231 10.1146/annurev-biochem-010909-095056
15. C. Kuscu, S. Arslan, R. Singh, J. Thorpe, and M. Adli Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease Nature Biotechnology 32 7 2014 677 683 10.1038/nbt.2916
16. P. Mali, J. Aach, P.B. Stranges, K.M. Esvelt, M. Moosburner, and S. Kosuri CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering Nature Biotechnology 31 9 2013 833 838 10.1038/nbt.2675
17. F.J. Mojica, C. Diez-Villasenor, J. Garcia-Martinez, and C. Almendros Short motif sequences determine the targets of the prokaryotic CRISPR defence system Microbiology 155 Pt. 3 2009 733 740 10.1099/mic.0.023960-0
18. H. Nishimasu, F.A. Ran, P.D. Hsu, S. Konermann, S.I. Shehata, and N. Dohmae Crystal structure of cas9 in complex with guide RNA and target DNA Cell 156 5 2014 935 949 10.1016/j.cell.2014.02.001
19. V. Pattanayak, S. Lin, J.P. Guilinger, E. Ma, J.A. Doudna, and D.R. Liu High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity Nature Biotechnology 31 9 2013 839 843 10.1038/nbt.2673
20. F.A. Ran, P.D. Hsu, C.Y. Lin, J.S. Gootenberg, S. Konermann, and A.E. Trevino Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity Cell 154 6 2013 1380 1389 10.1016/j.cell.2013.08.021
21. H. Wang, H. Yang, C.S. Shivalila, M.M. Dawlaty, A.W. Cheng, and F. Zhang One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering Cell 153 4 2013 910 918 10.1016/j.cell.2013.04.025
22. B. Wiedenheft, S.H. Sternberg, and J.A. Doudna RNA-guided genetic silencing systems in bacteria and archaea Nature 482 7385 2012 331 338 10.1038/nature10886
23. X. Wu, D.A. Scott, A.J. Kriz, A.C. Chiu, P.D. Hsu, and D.B. Dadon Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells Nature Biotechnology 2014 10.1038/nbt.2889