Generation of site-specific mutations in the rat genome via CRISPR/Cas9

Methods in Enzymology

Volumn 546, Issue C, 2014, Pages 297-317

  Guan, Yuting ^a   Shao, Yanjiao ^a   Li, Dali ^a   Liu, Mingyao ^a  

^a EAST CHINA NORMAL UNIVERSITY   (China)

Author keywords

Gene editing; Knockout Knockin; Mutation; One cell embryo; Rat genome

Indexed keywords

CRISPR ASSOCIATED PROTEIN; CRISPR ASSOCIATED PROTEIN 9; MESSENGER RNA; OLIGONUCLEOTIDE; RNA; SINGLE GUIDE RNA; UNCLASSIFIED DRUG; ENDONUCLEASE;

ADULT; ANIMAL EXPERIMENT; ARTICLE; CRISPR CAS SYSTEM; EMBRYO; EMBRYO TRANSFER; EXPERIMENTAL RAT; FEMALE; GENE MUTATION; GENE TARGETING; GENETIC ENGINEERING; GENOME; IN VITRO STUDY; KNOCKOUT RAT; MALE; MICROINJECTION; NONHUMAN; PREGNANCY; PRIORITY JOURNAL; RAT; RAT EMBRYO; RAT MUTANT; RNA TRANSCRIPTION; SITE DIRECTED MUTAGENESIS; ANIMAL; ANIMAL EMBRYO; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CYTOLOGY; GENETICS; METABOLISM; MUTAGENESIS; MUTATION; PROCEDURES;

MAMMALIA; RATTUS; RATTUS NORVEGICUS;

ANIMALS; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-ASSOCIATED PROTEINS; CRISPR-CAS SYSTEMS; EMBRYO, MAMMALIAN; ENDONUCLEASES; FEMALE; GENE TARGETING; GENOME; MUTAGENESIS; MUTATION; RATS;

EID: 84925411111     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-801185-0.00014-3     Document Type: Chapter

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