Visualizing notch signaling in vivo in Drosophila tissues

Methods in Molecular Biology

Volumn 1187, Issue , 2014, Pages 101-113

  Housden, Benjamin E ^a,b   Li, Jinghua ^b   Bray, Sarah J ^b  

^a HARVARD MEDICAL SCHOOL   (United States)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

Antibodies; Fluorescent reporters; Immunostaining; Notch reporters; Notch signaling; Transcriptional reporters; X gal staining

Indexed keywords

ANIMAL TISSUE; ARTICLE; DROSOPHILA; IMMUNOHISTOCHEMISTRY; IN VIVO STUDY; NONHUMAN; NOTCH SIGNALING; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; X GAL STAINING; ANIMAL; METABOLISM; MICROSCOPY; PROCEDURES; ULTRASTRUCTURE;

DROSOPHILA PROTEIN; NOTCH PROTEIN, DROSOPHILA; NOTCH RECEPTOR;

ANIMALS; DROSOPHILA; DROSOPHILA PROTEINS; IMMUNOHISTOCHEMISTRY; MICROSCOPY; RECEPTORS, NOTCH; SIGNAL TRANSDUCTION;

EID: 84925283072     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-1139-4_8     Document Type: Article

References (45)

1. Bray SJ (2006) Notch signalling: a simple pathway becomes complex. Nat Rev Mol Cell Biol 7:678-689
2. Fryer CJ, Lamar E, Turbachova I et al (2002) Mastermind mediates chromatin-specifi c transcription and turnover of the Notch enhancer complex. Genes Dev 16:1397-1411
3. Fryer CJ, White JB, Jones KA (2004) Mastermind recruits CycC:CDK8 to phosphorylate the Notch ICD and coordinate activation with turnover. Mol Cell 16: 509-520
4. Schroeter EH, Kisslinger JA, Kopan R (1998) Notch-1 signalling requires ligand-induced proteolytic release of intracellular domain. Nature 393:382-386
5. Micchelli CA, Rulifson EJ, Blair SS (1997) The function and regulation of cut expression on the wing margin of Drosophila: Notch, wingless and a dominant negative role for Delta and Serrate. Development 124:1485-1495
6. Bailey AM, Posakony JW (1995) Suppressor of hairless directly activates transcription of enhancer of split complex genes in response to Notch receptor activity. Genes Dev 9: 2609-2622
7. Jennings B, Preiss A, Delidakis C et al (1994) The Notch signalling pathway is required for Enhancer of split bHLH protein expression during neurogenesis in the Drosophila embryo. Development 120:3537-3548
8. Lai EC, Bodner R, Posakony JW (2000) The enhancer of split complex of Drosophila includes four Notch-regulated members of the bearded gene family. Development 127: 3441-3455
9. Lecourtois M, Schweisguth F (1995) The neurogenic suppressor of hairless DNA-binding protein mediates the transcriptional activation of the enhancer of split complex genes triggered by Notch signaling. Genes Dev 9:2598-2608
10. Wech I, Bray S, Delidakis C et al (1999) Distinct expression patterns of different enhancer of split bHLH genes during embryogenesis of Drosophila melanogaster. Dev Genes Evol 209:370-375
11. de Celis JF, de Celis J, Ligoxygakis P et al (1996) Functional relationships between Notch, Su(H) and the bHLH genes of the E(spl) complex: the E(spl) genes mediate only a subset of Notch activities during imaginal development. Development 122:2719-2728
12. Nellesen DT, Lai EC, Posakony JW (1999) Discrete enhancer elements mediate selective responsiveness of enhancer of split complex genes to common transcriptional activators. Dev Biol 213:33-53
13. Furriols M, Bray S (2001) A model Notch response element detects suppressor of hairless-dependent molecular switch. Curr Biol 11:60-64
14. Housden BE, Millen K, Bray SJ (2012) Drosophila reporter vectors compatible with phiC31 integrase transgenesis techniques and their use to generate new Notch reporter fl y lines. G3 Bethesda 2:79-82
15. Saj A, Arziman Z, Stempfl e D et al (2010) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Dev Cell 18:862-876
16. Go MJ, Eastman DS, Artavanis-Tsakonas S (1998) Cell proliferation control by Notch signaling in Drosophila development. Development 125:2031-2040
17. Li X, Zhao X, Fang Y et al (1998) Generation of destabilized green fl uorescent protein as a transcription reporter. J Biol Chem 273: 34970-34975
18. Presente A, Shaw S, Nye JS et al (2002) Transgene-mediated RNA interference defi nes a novel role for notch in chemosensory startle behavior. Genesis 34:165-169
19. Helms W, Lee H, Ammerman M et al (1999) Engineered truncations in the Drosophila mastermind protein disrupt Notch pathway function. Dev Biol 215:358-374
20. McGuire SE, Le PT, Osborn AJ et al (2003) Spatiotemporal rescue of memory dysfunction in Drosophila. Science 302:1765-1768
21. Brand AH, Perrimon N (1993) Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development 118:401-415
22. Xu T, Rubin GM (1993) Analysis of genetic mosaics in developing and adult Drosophila tissues. Development 117:1223-1237
23. Lee T, Luo L (1999) Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis. Neuron 22: 451-461
24. Classen AK, Aigouy B, Giangrande A et al (2008) Imaging Drosophila pupal wing morphogenesis. Methods Mol Biol 420:265-275
25. Muller HA (2008) Immunolabeling of embryos. Methods Mol Biol 420:207-218
26. Stocker H, Gallant P (2008) Getting started : an overview on raising and handling Drosophila. Methods Mol Biol 420:27-44
27. Cooper MT, Tyler DM, Furriols M et al (2000) Spatially restricted factors cooperate with notch in the regulation of Enhancer of split genes. Dev Biol 221:390-403
28. de Celis JF, Tyler DM, de Celis J et al (1998) Notch signalling mediates segmentation of the Drosophila leg. Development 125: 4617-4626
29. Pines MK, Housden BE, Bernard F et al (2010) The cytolinker Pigs is a direct target and a negative regulator of Notch signalling. Development 137:913-922
30. Assa-Kunik E, Torres IL, Schejter ED et al (2007) Drosophila follicle cells are patterned by multiple levels of Notch signaling and antagonism between the Notch and JAK/STAT pathways. Development 134:1161-1169
31. Almeida MS, Bray SJ (2005) Regulation of post-embryonic neuroblasts by Drosophila Grainyhead. Mech Dev 122:1282-1293
32. Cooper MT, Bray SJ (1999) Frizzled regulation of Notch signalling polarizes cell fate in the Drosophila eye. Nature 397:526-530
33. Kramatschek B, Campos-Ortega JA (1994) Neuroectodermal transcription of the Drosophila neurogenic genes E(spl) and HLHm5 is regulated by proneural genes. Development 120:815-826
34. Castro B, Barolo S, Bailey AM et al (2005) Lateral inhibition in proneural clusters: cis-regulatory logic and default repression by suppressor of hairless. Development 132:3333-3344
35. Barolo S, Castro B, Posakony JW (2004) New Drosophila transgenic reporters: insulated P-element vectors expressing fast-maturing RFP. Biotechniques 36:436-440
36. Afek Y, Alon N, Barad O et al (2011) A biological solution to a fundamental distributed computing problem. Science 331:183-185
37. Williams JA, Paddock SW, Vorwerk K et al (1994) Organization of wing formation and induction of a wing-patterning gene at the dorsal/ventral compartment boundary. Nature 368:299-305
38. Djiane A, Krejci A, Bernard F et al (2013) Dissecting the mechanisms of Notch induced hyperplasia. EMBO J 32:60-71
39. Blochlinger K, Bodmer R, Jack J et al (1988) Primary structure and expression of a product from cut, a locus involved in specifying sensory organ identity in Drosophila. Nature 333:629-635
40. Brook WJ, Cohen SM (1996) Antagonistic interactions between wingless and decapentaplegic responsible for dorsal-ventral pattern in the Drosophila Leg. Science 273: 1373-1377
41. Yip ML, Lamka ML, Lipshitz HD (1997) Control of germ-band retraction in Drosophila by the zinc-fi nger protein HINDSIGHT. Development 124:2129-2141
42. Giráldez AJ, Pérez L, Cohen SM (2002) A naturally occurring alternative product of the mastermind locus that represses notch signalling. Mech Dev 115:101-105
43. Lieber T, Kidd S, Alcamo E et al (1993) Antineurogenic phenotypes induced by truncated Notch proteins indicate a role in signal transduction and may point to a novel function for Notch in nuclei. Genes Dev 7:1949-1965
44. Seugnet L, Simpson P, Haenlin M (1997) Requirement for dynamin during Notch signaling in Drosophila neurogenesis. Dev Biol 192:585-598
45. Rebay I, Fehon RG, Artavanis-Tsakonas S (1993) Specifi c truncations of Drosophila Notch defi ne dominant activated and dominant negative forms of the receptor. Cell 74:319-329