Efficient, validated method for detection of mycobacterial growth in liquid culture media by use of bead beating, magnetic-particle-based nucleic acid isolation, and quantitative PCR

Journal of Clinical Microbiology

Volumn 53, Issue 4, 2015, Pages 1121-1128

  Plain, Karren M ^a   Waldron, Anna M ^a   Begg, Douglas J ^a   De Silva, Kumudika ^a   Purdie, Auriol C ^a   Whittington, Richard J ^a  

^a UNIVERSITY OF SYDNEY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOL; BACTERIAL DNA; CULTURE MEDIUM;

ANIMAL TISSUE; ARTICLE; BACTERIAL CELL WALL; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM DETECTION; BACTERIUM EXAMINATION; BEAD BEATING; BOVINAE; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST ACCURACY STUDY; DNA ISOLATION; EGG YOLK; FECES ANALYSIS; LIMIT OF DETECTION; LIQUID CULTURE; MYCOBACTERIUM; NONHUMAN; PARATUBERCULOSIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SENSITIVITY ANALYSIS; SHEEP; ANIMAL; BOVINE; CHEMISTRY; CULTURE MEDIUM; FECES; GENETICS; ISOLATION AND PURIFICATION; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS; PROCEDURES; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY;

BOS; CORYNEBACTERINEAE; MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS; MYCOBACTERIUM TUBERCULOSIS; OVIS ARIES;

ANIMALS; BACTERIOLOGICAL TECHNIQUES; CATTLE; CULTURE MEDIA; DNA, BACTERIAL; FECES; LIMIT OF DETECTION; MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS; PARATUBERCULOSIS; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SHEEP;

EID: 84925276059     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.03521-14     Document Type: Article

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