Development and validation of a liquid medium (M7H9C) for routine culture of Mycobacterium avium subsp. paratuberculosis to replace modified bactec 12B medium

Journal of Clinical Microbiology

Volumn 51, Issue 12, 2013, Pages 3993-4000

  Whittington, Richard J ^a   Whittington, Ann Michele ^a   Waldron, Anna ^a   Begg, Douglas J ^a   De Silva, Kumi ^a   Purdie, Auriol C ^a   Plain, Karren M ^a  

^a UNIVERSITY OF SYDNEY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

ALBUMIN; CATALASE; GLUCOSE; MACROGOL STEARATE;

ANIMAL TISSUE; ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; CATTLE; CONTROLLED STUDY; EGG YOLK; FECES ANALYSIS; LIMIT OF DETECTION; LIQUID CULTURE; MYCOBACTERIUM PARATUBERCULOSIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SENSITIVITY ANALYSIS; SHEEP; VALIDATION STUDY;

ANIMALS; AUSTRALIA; BACTERIOLOGICAL TECHNIQUES; CATTLE; CATTLE DISEASES; COSTS AND COST ANALYSIS; CULTURE MEDIA; FECES; MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS; NEW ZEALAND; PARATUBERCULOSIS; SHEEP; SHEEP DISEASES; UNITED STATES;

EID: 84889833137     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.01373-13     Document Type: Article

References (44)

1. Ott SL, Wells SJ, Wagner BA. 1999. Herd-level economic losses associated with Johne's disease on US dairy operations. Prev. Vet. Med. 40:179-192.
2. Forde T, Kutz S, De Buck J, Warren A, Ruckstuhl K, Pybus M, Orsel K. 2012. Occurrence, diagnosis, and strain typing of Mycobacterium avium subspecies paratuberculosis infection in rocky mountain bighorn sheep (Ovis canadensis canadensis) in southwestern Alberta. J. Wildl. Dis. 48:1-11.
3. Kirkwood CD, Wagner J, Boniface K, Vaughan J, Michalski WP, Catto-Smith AG, Cameron DJ, Bishop RF. 2009. Mycobacterium avium subspecies paratuberculosis in children with early-onset Crohn's disease. Inflamm. Bowel Dis. 15:1643-1655.
4. Collins MT, Kenefeck KB, Sockett DC, Lambrecht RS, McDonald J, Jorgensen JB. 1990. Enhanced radiometric detection of Mycobacterium paratuberculosis by using filter-concentrated bovine fecal specimens. J. Clin. Microbiol. 28:2514-2519.
5. Eamens GJ, Whittington RJ, Marsh IB, Turner MJ, Saunders V, Kemsley PD, Rayward D. 2000. Comparative sensitivity of various faecal culture methods and ELISA in dairy cattle herds with endemic Johne's disease. Vet. Microbiol. 77:357-367.
6. Motiwala AS, Strother M, Theus NE, Stich RW, Byrum B, Shulaw WP, Kapur V, Sreevatsan S. 2005. Rapid detection and typing of strains of Mycobacterium avium subsp. paratuberculosis from broth cultures. J. Clin. Microbiol. 43:2111-2117.
7. Stich RW, Byrum B, Love B, Theus N, Barber L, Shulaw WP. 2004. Evaluation of an automated system for non-radiometric detection of Mycobacterium avium paratuberculosis in bovine feces. J. Microbiol. Methods 56:267-275.
8. Cousins DV, Evans RJ, Francis BR. 1995. Use of BACTEC radiometric culture method and polymerase chain reaction for the rapid screening of faeces and tissues for Mycobacterium paratuberculosis. Aust. Vet. J. 72: 458-462.
9. Damato JJ, Collins MT. 1990. Growth of Mycobacterium paratuberculosis in radiometric, Middlebrook and egg-based media. Vet. Microbiol. 22:31-42.
10. Whittington R. 2010. Cultivation of Mycobacterium avium subsp. paratuberculosis, p 244-266. In Behr MA, Collins DM (ed), Paratuberculosis. Organism, disease, control. CABI, Wallingford, United Kingdom.
11. Collins DM, Gabric DM, de Lisle GW. 1990. Identification of two groups of Mycobacterium paratuberculosis strains by restriction endonuclease analysis and DNA hybridization. J. Clin. Microbiol. 28:1591-1596.
12. Gumber S, Whittington RJ. 2007. Comparison of BACTEC 460 and MGIT 960 systems for the culture of Mycobacterium avium subsp. paratuberculosis S strain and observations on the effect of inclusion of ampicillin in culture media to reduce contamination. Vet. Microbiol. 119:42-52.
13. Dimareli-Malli Z, Mazaraki K, Stevenson K, Tsakos P, Zdragas A, Giantzi V, Petridou E, Heron I, Vafeas G. 2013. Culture phenotypes and molecular characterization of Mycobacterium avium subsp. paratuberculosis isolates from small ruminants. Res. Vet. Sci. 95:49-53.
14. Whittington RJ, Marsh I, McAllister S, Turner MJ, Marshall DJ, Fraser CA. 1999. Evaluation of modified BACTEC 12B radiometric medium and solid media for culture of Mycobacterium avium subsp. paratuberculosis from sheep. J. Clin. Microbiol. 37:1077-1083.
15. Whittington RJ, Marsh IB, Saunders V, Grant IR, Juste R, Sevilla IA, Manning EJ, Whitlock RH. 2011. Culture phenotypes of genomically and geographically diverse Mycobacterium avium subsp. paratuberculosis isolates from different hosts. J. Clin. Microbiol. 49:1822-1830.
16. Pozzato N, Gwozdz J, Gastaldelli M, Capello K, Dal Ben C, Stefani E. 2011. Evaluation of a rapid and inexpensive liquid culture system for the detection of Mycobacterium avium subsp. paratuberculosis in bovine faeces. J. Microbiol. Methods 84:413-417.
17. Marsh IB, Bannantine JP, Paustian ML, Tizard ML, Kapur V, Whit tington RJ. 2006. Genomic comparison of Mycobacterium avium subsp. paratuberculosis sheep and cattle strains by microarray hybridization. J. Bacteriol. 188:2290-2293.
18. Marsh I, Whittington R, Cousins D. 1999. PCR-restriction endonuclease analysis for identification and strain typing of Mycobacterium avium subsp. paratuberculosis and M. avium subsp. avium based on polymorphisms in IS1311. Mol. Cell. Probes 13:115-126.
19. Cousins DV, Whittington R, Marsh I, Masters A, Evans RJ, Kluver P. 1999. Mycobacteria distinct from Mycobacterium avium subsp. paratuberculosis isolated from the faeces of ruminants possess IS900-like sequences detectable by IS900 polymerase chain reaction: implications for diagnosis. Mol. Cell. Probes 13:431-442.
20. Reddacliff LA, Nicholls PJ, Vadali A, Whittington RJ. 2003. Use of growth indices from radiometric culture for quantification of sheep strains of Mycobacterium avium subsp. paratuberculosis. Appl. Environ. Microbiol. 69:3510-3516.
21. Motulsky H. 1995. Intuitive biostatistics: a nonmathematical guide to statistical thinking. Oxford University Press, Inc., New York, NY.
22. Fleiss JL. 1981. Statistical methods for rates and proportions, 2nd ed. John Wiley, New York, NY. 23.
23. Reddacliff LA, Vadali A, Whittington RJ. 2003. The effect of decontamination protocols on the numbers of sheep strain Mycobacterium avium subsp. paratuberculosis isolated from tissues and faeces. Vet. Microbiol. 95:271-282.
24. Grant IR, Kirk RB, Hitchings E, Rowe MT. 2003. Comparative evaluation of the MGIT and BACTEC culture systems for the recovery of Mycobacterium avium subsp. paratuberculosis from milk. J. Appl. Microbiol. 95:196-201.
25. Ellingson JLE, Koziczkowski JJ, Anderson JL. 2004. Comparison of PCR prescreening to two cultivation procedures with PCR confirmation for detection of Mycobacterium avium subsp. paratuberculosis in U.S. Department of Agriculture fecal check test samples. J. Food Prot. 67:2310-2314.
26. Liu Y-C, Huang T-S, Huang W-K. 1999. Comparison of a nonradiometric liquid-medium method (MB REDOX) with the BACTEC system for growth and identification of mycobacteria in clinical specimens. J. Clin. Microbiol. 37:4048-4050.
27. Thorel MF, Krichevsky M, Lévy-Frébault VV. 1990. Numerical taxonomy of mycobactin-dependent mycobacteria, emended description of Mycobacterium avium, and description of Mycobacterium avium subsp. avium subsp. nov., Mycobacterium avium subsp. paratuberculosis subsp. nov., and Mycobacterium avium subsp. silvaticum subsp. nov. Int. J. Syst. Bacteriol. 40:254-260.
28. Whittington RJ. 2009. Factors affecting isolation and identification of Mycobacterium avium subsp. paratuberculosis from faecal and tissue samples in a liquid culture system. J. Clin. Microbiol. 47:614-622.
29. Shin SJ, Anklam K, Manning EJ, Collins MT. 2009. Rapid mycobacterial liquid culture-screening method for Mycobacterium avium complex based on secreted antigen-capture enzyme-linked immunosorbent assay. Clin. Vaccine Immunol. 16:613-620.
30. Lambrecht RS, Carriere JF, Collins MT. 1988. A model for analyzing growth kinetics of a slowly growing Mycobacterium sp. Appl. Environ. Microbiol. 54:910-916.
31. Shin SJ, Han JH, Manning EJ, Collins MT. 2007. Rapid and reliable method for quantification of Mycobacterium paratuberculosis by use of the BACTEC MGIT 960 system. J. Clin. Microbiol. 45:1941-1948.
32. Cutler R, Wilson P, Clarke F. 1987. The effect of polyoxyethylene stearate (POES) on the growth of mycobacteria in radiometric 7H12 Middlebrook TB medium. Tubercle 68:209-220.
33. Stinson MW, Solotorovsky M. 1971. Interaction of Tween 80 detergent with mycobacteria in synthetic medium. I. Effect of Tween 80 on growth and turbidimetric response of Mycobacterium avium cultures. Am. Rev. Respir. Dis. 104:717-727.
34. van Boxtel RM, Lambrecht RS, Collins MT. 1990. Effect of polyoxyethylene sorbate compounds (Tweens) on colonial morphology, growth, and ultrastructure of Mycobacterium paratuberculosis. APMIS 98:901-908.
35. Kaur R, Grant D, Eaves T. 1980. Comparison of polyethylene glycol and polyoxyethylene stearate as excipients for solid dispersion systems of griseofulvin and tolbutamide II: dissolution and solubility studies. J. Pharm. Sci. 69:1321-1326.
36. Realini L, van der Stuyft P, De Ridder K, Hirschel B, Portaels F. 1997. Inhibitory effects of polyoxyethylene stearate, PANTA, and neutral pH on growth of Mycobacterium genavense in BACTEC primary cultures. J. Clin. Microbiol. 35:2791-2794.
37. Bull TJ, McMinn EJ, Sidi-Boumedine K, Skull A, Durkin D, Neild P, Rhodes G, Pickup R, Hermon-Taylor J. 2003. Detection and verification of Mycobacterium avium subsp. paratuberculosis in fresh ileocolonic mucosal biopsy specimens from individuals with and without Crohn's disease. J. Clin. Microbiol. 41:2915-2923.
38. Eamens GJ, Walker DM, Porter NS, Fell SA. 2007. Pooled faecal culture for the detection of Mycobacterium avium subsp paratuberculosis in goats. Aust. Vet. J. 85:243-251.
39. Eamens GJ, Walker DM, Porter NS, Fell SA. 2008. Radiometric pooled faecal culture for the detection of Mycobacterium avium subsp. paratuberculosis in low-shedder cattle. Aust. Vet. J. 86:259-265.
40. Whittington RJ, Fell S, Walker D, McAllister S, Marsh I, Sergeant E, Taragel CA, Marshall DJ, Links IJ. 2000. Use of pooled fecal culture for sensitive and economic detection of Mycobacterium avium subsp. paratuberculosis infection in flocks of sheep. J. Clin. Microbiol. 38:2550-2556.
41. Whittington RJ, Sergeant ES. 2001. Progress towards understanding the spread, detection and control of Mycobacterium avium subsp. paratuberculosis in animal populations. Aust. Vet. J. 79:267-278.
42. Pillars RB, Grooms DL, Woltanski JA, Blair E. 2009. Prevalence of Michigan dairy herds infected with Mycobacterium avium subspecies paratuberculosis as determined by environmental sampling. Prev. Vet. Med. 89:191-196.
43. Dhand NK, Sergeant E, Toribio JA, Whittington RJ. 2010. Estimation of sensitivity and flock-sensitivity of pooled faecal culture for Mycobacterium avium subsp. paratuberculosis in sheep. Prev. Vet. Med. 95:248-257.
44. Zimbro MJ, Power DA. 2003. Difco and BBL manual: manual of microbiological culture media. Becton, Dickinson and Company, Sparks, MD.