Rapid detection of Gram-negative bacteria and their drug resistance genes from positive blood cultures using an automated microarray assay

Diagnostic Microbiology and Infectious Disease

Volumn 81, Issue 3, 2015, Pages 153-157

  Han, Eunhee ^a,b   Park, Dong Jin ^a,b   Kim, Yukyoung ^a,b   Yu, Jin Kyung ^a,b   Park, Kang Gyun ^a,b   Park, Yeon Joon ^a,b  

^a The Catholic University of Korea   (South Korea)

^b The Catholic University of Korea College of Medicine   (South Korea)

Author keywords

Automated microarray assay; Polymicrobial; Positive blood cultures; Verigene

Indexed keywords

BETA LACTAMASE CTX M;

ACCURACY; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; BLACTX M GENE; BLAIMP GENE; BLAKPC GENE; BLANDM GENE; BLAOXA GENE; BLAVIM GENE; BLOOD CULTURE; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST ACCURACY STUDY; ESCHERICHIA COLI; EVALUATION STUDY; GRAM NEGATIVE BACTERIUM; GRAM NEGATIVE BLOOD CULTURE ASSAY; GRAM NEGATIVE SEPSIS; HUMAN; IN VITRO STUDY; INTERMETHOD COMPARISON; MICROARRAY KIT; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; RAPID TEST; RESISTANCE GENE; SENSITIVITY AND SPECIFICITY; TURNAROUND TIME; BLOOD; DRUG EFFECTS; GENETICS; GRAM-NEGATIVE BACTERIAL INFECTIONS; ISOLATION AND PURIFICATION; LABORATORY AUTOMATION; MICROARRAY ANALYSIS; MICROBIOLOGY; MOLECULAR DIAGNOSIS; PROCEDURES; TIME;

AUTOMATION, LABORATORY; BLOOD; DRUG RESISTANCE, BACTERIAL; GRAM-NEGATIVE BACTERIA; GRAM-NEGATIVE BACTERIAL INFECTIONS; HUMANS; MICROARRAY ANALYSIS; MOLECULAR DIAGNOSTIC TECHNIQUES; TIME FACTORS;

EID: 84925036354     PISSN: 07328893     EISSN: 18790070     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2014.10.009     Document Type: Article

References (38)

1. Arakawa Y., Shibata N., Shibayama K., Kurokawa H., Yagi T., Fujiwara H., et al. Convenient test for screening metallo-beta-lactamase-producing gram-negative bacteria by using thiol compounds. J Clin Microbiol 2000, 38(1):40-43.
2. Bhatti M.M., Boonlayangoor S., Beavis K.G., Tesic V. Evaluation of FilmArray and Verigene systems for rapid identification of positive blood cultures. J Clin Microbiol 2014, 52(9):3433-3436.
3. Bradford P.A., Bratu S., Urban C., Visalli M., Mariano N., Landman D., et al. Emergence of carbapenem-resistant Klebsiella species possessing the class A carbapenem-hydrolyzing KPC-2 and inhibitor-resistant TEM-30 beta-lactamases in New York City. Clin Infect Dis 2004, 39(1):55-60.
4. Chong Y., Ito Y., Kamimura T. Genetic evolution and clinical impact in extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae. Infect Genet Evol 2011, 11(7):1499-1504.
5. Clinical and Laboratory Standards Institute (CLSI) Performance standards for antimicrobial susceptibility testing; 23rd informational supplement 2013, CLSI, Wayne, PA.
6. Dellinger R.P., Levy M.M., Rhodes A., Annane D., Gerlach H., Opal S.M., et al. Surviving sepsis campaign: international guidelines for management of severe sepsis and septic shock, 2012. Intensive Care Med 2013, 39(2):165-228.
7. Dodémont M., De Mendonça R., Nonhoff C., Roisin S., Denis O. Performance of the Verigene gram-negative blood culture assay for rapid detection of bacteria and resistance determinants. J Clin Microbiol 2014, 52(8):3085-3087.
8. Drancourt M., Boolet C., Carta A., Rousselier P. Phylogenetic analyses of Klebsiella species delineate Klebsiella and Raoultella gen. nov., with description of Raoultella ornithinolytica comb. nov., Raoultella terrigena comb. nov. and Raoultella planticola comb. nov. Int J Syst Evol Microbiol 2001, 51(3):925-932.
9. Hochedez P., Hope-Rapp E., Olive C., Nicolas M., Beaucaire G., Cabié A. Bacteremia caused by Aeromonas species [corrected] complex in the Caribbean Islands of Martinique and Guadeloupe. Am J Trop Med Hyg 2010, 83(5):1123-1127.
10. Jacoby G.A. AmpC beta-lactamases. Clin Microbiol Rev 2009, 22(1):161-182.
11. Kang C.I., Kim S.H., Park W.B., Lee K.D., Kim H.B., Kim E.C., et al. Bloodstream infections caused by antibiotic-resistant gram-negative bacilli: risk factors for mortality and impact of inappropriate initial antimicrobial therapy on outcome. Antimicrob Agents Chemother 2005, 49(2):760-766.
12. Kim J., Kwon Y., Pai H., Kim J.W., Cho D.T. Survey of Klebsiella pneumoniae strains producing extended-spectrum beta-lactamases: prevalence of SHV-12 and SHV-2a in Korea. J Clin Microbiol 1998, 36(5):1446-1449.
13. Kim M., Heo S.R., Choi S.H., Kwon H., Park J.S., Seong M.W., et al. Comparison of the MicroScan, VITEK 2, and Crystal GP with 16S rRNA sequencing and MicroSeq 500 v2.0 analysis for coagulase-negative staphylococci. BMC Microbiol 2008, 8:233. 10.1186/1471-2180-8-233.
14. Ko W.C., Lee H.C., Chuang Y.C., Liu C.C., Wu J.J. Clinical features and therapeutic implications of 104 episodes of monomicrobial Aeromonas bacteraemia. J Infect 2000, 40(3):267-273.
15. Kumar A., Roberts D., Wood K.E., Light B., Parrillo J.E., Sharma S., et al. Duration of hypotension before initiation of effective antimicrobial therapy is the critical determinant of survival in human septic shock. Crit Care Med 2006, 34(6):1589-1596.
16. Labarca J.A., Salles M.J., Seas C., Guzmán-Blanco M. Carbapenem resistance in Pseudomonas aeruginosa and Acinetobacter baumannii in the nosocomial setting in Latin America. Crit Rev Microbiol 2014, 27:1-17.
17. Lee K., Yong D., Yum J.H., Kim H.H., Chong Y. Diversity of TEM-52 extended-spectrum beta-lactamase-producing non-typhoidal Salmonella isolates in Korea. J Antimicrob Chemother 2003, 52:493-496.
18. Leggieri N., Rida A., François P., Schrenzel J. Molecular diagnosis of bloodstream infections: planning to (physically) reach the bedside. Curr Opin Infect Dis 2010, 23(4):311-319.
19. Livermore D.M., Canton R., Gniadkowski M., Nordmann P., Rossolini G.M., Arlet G., et al. CTX-M: changing the face of ESBLs in Europe. J Antimicrob Chemother 2007, 59(2):165-174.
20. Mancini N., Infurnari L., Ghidoli N., Valzano G., Clementi N., Burioni R., et al. Potential impact of a microarray-based nucleic acid assay for rapid detection of Gram-negative bacteria and resistance markers in positive blood cultures. J Clin Microbiol 2014, 52(4):1242-1245.
21. Martin G.S., Mannino D.M., Eaton S., Moss M. The epidemiology of sepsis in the United States from 1979 through 2000. N Engl J Med 2003, 348(16):1546-1554.
22. Morgan M., Marlowe E., Della-Latta P., Salimnia H., Novak-Weekley S., et al. Multicenter evaluation of a new shortened peptide nucleic acid fluorescence in situ hybridization procedure for species identification of select Gram-negative bacilli from blood cultures. J Clin Microbiol 2010, 48(6):2268-2270.
23. Djahmi N., Dunyach-Remy C., Pantel A., Dekhil M., Sotto A., Lavigne J.P. Epidemiology of carbapenemase-producing Enterobacteriaceae and Acinetobacter baumannii in Mediterranean countries. Biomed Res Int 2014, 2014:305784. 10.1155/2014/305784.
24. Nüesch-Inderbinen M.T., Hächler H., Kayser F.H. Detection of genes coding for extended-spectrum SHV beta-lactamases in clinical isolates by a molecular genetic method, and comparison with the E test. Eur J Clin Microbiol Infect Dis 1996, 15(5):398-402.
25. Östholm B.Å., Tärnberg M., Monstein H.J., Hällgren A., Hanberger H., Nilsson L.E. High frequency of co-resistance in CTX-M-producing Escherichia coli to non-beta-lactam antibiotics, with the exceptions of amikacin, nitrofurantoin, colistin, tigecycline, and fosfomycin, in a county of Sweden. Scand J Infect Dis 2013, 45(4):271-278.
26. Park Y.J., Yu J.K., Park K.G., Park Y.G., Lee S., Kim S.Y., et al. Prevalence and contributing factors of nonsusceptibility to imipenem or meropenem in extended-spectrum β-lactamase-producing Klebsiella pneumoniae and Escherichia coli. Diagn Microbiol Infect Dis 2011, 71(1):87-89.
27. Poirel L., Lambert T., Türkoglü S., Ronco E., Gaillard J., Nordmann P. Characterization of Class 1 integrons from Pseudomonas aeruginosa that contain the bla(VIM-2) carbapenem-hydrolyzing beta-lactamase gene and of two novel aminoglycoside resistance gene cassettes. Antimicrob Agents Chemother 2001, 45(2):546-552.
28. Poirel L., Héritier C., Tolün V., Nordmann P. Emergence of oxacillinase-mediated resistance to imipenem in Klebsiella pneumoniae. Antimicrob Agents Chemother 2004, 48(1):15-22.
29. Rello J., Quintana E., Mirelis B., Gurguí M., Net A., Prats G. Polymicrobial bacteremia in critically ill patients. Intensive Care Med 1993, 19(1):22-25.
30. Rossolini G.M., D'Andrea M.M., Mugnaioli C. The spread of CTX-M-type extended-spectrum beta-lactamases. Clin Microbiol Infect 2008, 14(Suppl. 1):33-41.
31. Song W., Bae I.K., Lee Y.N., Lee C.H., Lee S.H., Jeong S.H. Detection of extended-spectrum beta-lactamases by using boronic acid as an AmpC beta-lactamase inhibitor in clinical isolates of Klebsiella spp. and Escherichia coli. J Clin Microbiol 2007, 45(4):1180-1184.
32. Sullivan K.V., Deburger B., Roundtree S.S., Ventrola C.A., Blecker-Shelly D.L., Mortensen J.E., et al. Pediatric multicenter evaluation of the Verigene Gram-negative blood culture test for rapid detection of inpatient bacteremia involving Gram-negative organisms, extended-spectrum beta-lactamases, and carbapenemases. J Clin Microbiol 2014, 52(7):2416-2421.
33. Tang H.J., Lai C.C., Lin H.L., Chao C.M. Clinical manifestations of bacteremia caused by Aeromonas species in southern Taiwan. PLoS One 2014, 9(3):e91642. (doi: 10.1371).
34. Tojo M., Fujita T., Ainoda Y., Nagamatsu M., Hayakawa K., Mezaki K., et al. Evaluation of an automated rapid diagnostic assay for detection of gram-negative bacteria and their drug-resistance genes in positive blood cultures. PLoS One 2014, 9(4):e94064. (doi: 10.1371).
35. Wang J.H., Wang C.Y., Chi C.Y., Ho M.W., Ho C.M., Lin P.C. Clinical presentations, prognostic factors, and mortality in patients with Aeromonas sobria complex bacteremia in a teaching hospital: a 5-year experience. J Microbiol Immunol Infect 2009, 42(6):510-515.
36. Woodford N., Fagan E.J., Ellington M.J. Multiplex PCR for rapid detection of genes encoding CTX-M extended-spectrum (beta)-lactamases. J Antimicrob Chemother 2006, 57(1):154-155.
37. Woodford N., Ellington M.J., Coelho J.M., Turton J.F., Ward M.E., Brown S., et al. Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp. Int J Antimicrob Agents 2006, 27(4):351-353.
38. Yong D., Toleman M.A., Giske C.G., Cho H.S., Sundman K., Lee K., et al. Characterization of a new metallo-lactamase gene, blaNDM-1, and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14 from India. Antimicrob Agents Chemother 2009, 53(12):5046-5054.