Evaluation of an automated rapid diagnostic assay for detection of gram-negative bacteria and their drug-resistance genes in positive blood cultures

PLoS ONE

Volumn 9, Issue 4, 2014, Pages

  Tojo, Masayoshi ^a   Fujita, Takahiro ^b   Ainoda, Yusuke ^b   Nagamatsu, Maki ^a   Hayakawa, Kayoko ^a   Mezaki, Kazuhisa ^a   Sakurai, Aki ^c   Masui, Yoshinori ^c   Yazaki, Hirohisa ^a   Takahashi, Hiroshi ^d   Miyoshi Akiyama, Tohru ^a   Totsuka, Kyoichi ^b   Kirikae, Teruo ^a   Ohmagari, Norio ^a  

^a NATIONAL CENTER FOR GLOBAL HEALTH AND MEDICINE   (Japan)

^b TOKYO WOMEN'S MEDICAL UNIVERSITY   (Japan)

^c 1 7 1 Kohnodai   (Japan)

^d East West Diagnostics/Theranostics   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMIKACIN; AMOXICILLIN PLUS CLAVULANIC ACID; AMPICILLIN; AZTREONAM; CEFAZOLIN; CEFEPIME; CEFMETAZOLE; CEFOTAXIME; CEFOTIAM; CEFTAZIDIME; CIPROFLOXACIN; COLISTIN; COTRIMOXAZOLE; GENTAMICIN; IMIPENEM; LEVOFLOXACIN; MEROPENEM; MINOCYCLINE; PIPERACILLIN; PIPERACILLIN PLUS TAZOBACTAM; TIGECYCLINE; ANTIINFECTIVE AGENT;

ACINETOBACTER; ACINETOBACTER BAUMANNII; ACINETOBACTER OLEIVORANS; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM DETECTION; BACTERIUM ISOLATE; BLACTXM GENE; BLAIMP GENE; BLAKPC GENE; BLANDM GENE; BLAOXA23 GENE; BLAOXA24/40 GENE; BLAOXA48 GENE; BLAOXA58 GENE; BLAVIM GENE; BLOOD CULTURE; CITROBACTER FREUNDII; CONTROLLED STUDY; ESCHERICHIA COLI; GRAM NEGATIVE BACTERIUM; GRAM NEGATIVE SEPSIS; HUMAN; KLEBSIELLA OXYTOCA; KLEBSIELLA PNEUMONIAE; LIMIT OF DETECTION; NONHUMAN; PSEUDOMONAS AERUGINOSA; SENSITIVITY AND SPECIFICITY; SERRATIA MARCESCENS; CLASSIFICATION; DRUG EFFECTS; GENETICS; GRAM-NEGATIVE BACTERIAL INFECTIONS; ISOLATION AND PURIFICATION; MICROBIAL SENSITIVITY TEST; MICROBIOLOGY; MOLECULAR DIAGNOSIS; PROCEDURES;

ANTI-BACTERIAL AGENTS; DRUG RESISTANCE, BACTERIAL; GRAM-NEGATIVE BACTERIA; GRAM-NEGATIVE BACTERIAL INFECTIONS; HUMANS; MICROBIAL SENSITIVITY TESTS; MOLECULAR DIAGNOSTIC TECHNIQUES;

EID: 84899049190     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0094064     Document Type: Article

References (33)

1. Peleg AY, Hooper DC (2010) Hospital-acquired infections due to gram-negative bacteria. N Engl J Med 362: 1804-1813.
2. Shorr AF, Micek ST, Welch EC, Doherty JA, Reichley RM, et al. (2011) Inappropriate antibiotic therapy in Gram-negative sepsis increases hospital length of stay. Crit Care Med 39: 46-51.
3. Gaieski DF, Mikkelsen ME, Band RA, Pines JM, Massone R, et al. (2010) Impact of time to antibiotics on survival in patients with severe sepsis or septic shock in whom early goal-directed therapy was initiated in the emergency department. Crit Care Med 38: 1045-1053.
4. Kumar A, Roberts D, Wood KE, Light B, Parrillo JE, et al. (2006) Duration of hypotension before initiation of effective antimicrobial therapy is the critical determinant of survival in human septic shock. Crit Care Med 34: 1589-1596. (Pubitemid 43810035)
5. Versalovic J, Carroll KC, Funke G, Jorgensen JH, Landry ML, et al. (2011) Systems for Detection and Identification of Bacteria and yeasts. Manual of clinical microbiolgy. ASM Press, Washington, DC. pp. 15-26.
6. Queenan AM, Bush K (2007) Carbapenemases: the versatile beta-lactamases. Clin Microbiol Rev 20: 440-58, table of contents.
7. Liesenfeld O, Lehmann LE, Hunfeld K-P, Kost G (2014) Molecular Diagnosis of Sepsis: New aspects and recent developments. Eur J Microbiol Immunol: in press.
8. Mancini N, Clerici D, Diotti R, Perotti M, Ghidoli N, et al. (2008) Molecular diagnosis of sepsis in neutropenic patients with haematological malignancies. J Med Microbiol 57: 601-604. (Pubitemid 351632907)
9. Morgan M, Marlowe E, Della-Latta P, Salimnia H, Novak-Weekley S, et al. (2010) Multicenter evaluation of a new shortened peptide nucleic acid fluorescence in situ hybridization procedure for species identification of select Gram-negative bacilli from blood cultures. J Clin Microbiol 48: 2268-2270.
10. Leggieri N, Rida A, François P, Schrenzel J (2010) Molecular diagnosis of bloodstream infections: planning to (physically) reach the bedside. Curr Opin Infect Dis 23: 311-319.
11. Tissari P, Zumla A, Tarkka E, Mero S, Savolainen L, et al. (2010) Accurate and rapid identification of bacterial species from positive blood cultures with a DNA-based microarray platform: an observational study. Lancet 375: 224-230.
12. Samuel LP, Tibbetts RJ, Agotesku A, Fey M, Hensley R, et al. (2013) Evaluation of a microarray-based assay for rapid identification of Gram-positive organisms and resistance markers in positive blood cultures. J Clin Microbiol 51: 1188-1192.
13. Wojewoda CM, Sercia L, Navas M, Tuohy M, Wilson D, et al. (2013) Evaluation of the Verigene Gram-positive blood culture nucleic acid test for rapid detection of bacteria and resistance determinants. J Clin Microbiol 51: 2072-2076.
14. Buchan BW, Ginocchio CC, Manii R, Cavagnolo R, Pancholi P, et al. (2013) Multiplex identification of gram-positive bacteria and resistance determinants directly from positive blood culture broths: evaluation of an automated microarray-based nucleic acid test. PLoS Med 10: e1001478.
15. Sullivan KV, Turner NN, Roundtree SS, Young S, Brock-Haag CA, et al. (2013) Rapid detection of Gram-positive organisms by use of the Verigene Gram-positive blood culture nucleic acid test and the BacT/Alert Pediatric FAN system in a multicenter pediatric evaluation. J Clin Microbiol 51: 3579-3584.
16. Alby K, Daniels LM, Weber DJ, Miller MB (2013) Development of a treatment algorithm for streptococci and enterococci from positive blood cultures identified with the Verigene Gram-positive blood culture assay. J Clin Microbiol 51: 3869-3871.
17. Beal SG, Ciurca J, Smith G, John J, Lee F, et al. (2013) Evaluation of the nanosphere verigene gram-positive blood culture assay with the VersaTREK blood culture system and assessment of possible impact on selected patients. J Clin Microbiol 51: 3988-3992.
18. Sango A, McCarter YS, Johnson D, Ferreira J, Guzman N, et al. (2013) Stewardship approach for optimizing antimicrobial therapy through use of a rapid microarray assay on blood cultures positive for Enterococcus species. J Clin Microbiol 51: 4008-4011.
19. Mestas J, Polanco CM, Felsenstein S, Dien Bard J (2014) Performance of the Verigene Gram-positive blood culture assay for direct detection of Gram-positive organisms and resistance markers in a pediatric hospital. J Clin Microbiol 52: 283-287.
20. Kouyama Y, Harada S, Ishii Y, Saga T, Yoshizumi A, et al. (2012) Molecular characterization of carbapenem-non-susceptible Acinetobacter spp. in Japan: predominance of multidrug-resistant Acinetobacter baumannii clonal complex 92 and IMP-type metallo-β-lactamase-producing non-baumannii Acinetobacter species. J Infect Chemother 18: 522-528.
21. Sontakke S, Cadenas MB, Maggi RG, Diniz PPVP, Breitschwerdt EB (2009) Use of broad range16S rDNA PCR in clinical microbiology. J Microbiol Methods 76: 217-225.
22. Clinical Laboratory Standards Institute (2009) Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard M07-A7. 8th ed. Wayne, PA.
23. Zhang CX, Yang SY, Xu MX, Sun J, Liu H, et al. (2009) Serratia nematodiphila sp. nov., associated symbiotically with the entomopathogenic nematode Heterorhabditidoides chongmingensis (Rhabditida: Rhabditidae). Int J Syst Evol Microbiol 59: 1603-1608.
24. Seki M, Gotoh K, Nakamura S, Akeda Y, Yoshii T, et al. (2013) Fatal sepsis caused by an unusual Klebsiella species that was misidentified by an automated identification system. J Med Microbiol 62: 801-803.
25. Altun O, Almuhayawi M, Ullberg M, Ozenci V (2013) Clinical evaluation of the FilmArray blood culture identification panel in identification of bacteria and yeasts from positive blood culture bottles. J Clin Microbiol 51: 4130-4136.
26. Fujita S, Yosizaki K, Ogushi T, Uechi K, Takemori Y, et al. (2011) Rapid identification of gram-negative bacteria with and without CTX-M extended-spectrum β-lactamase from positive blood culture bottles by PCR followed by microchip gel electrophoresis. J Clin Microbiol 49: 1483-1488.
27. Cantón R, Coque TM (2006) The CTX-M beta-lactamase pandemic. Curr Opin Microbiol 9: 466-475.
28. Bush K, Jacoby GA (2010) Updated functional classification of beta-lactamases. Antimicrob Agents Chemother 54: 969-976.
29. European Centre for disease prevention and Control (ECDC) (2012) Healthcare-associated infections. Annual epidemiological report Reporting on 2010 surveillance data and 2011 epidemic intelligence data. p. 212.
30. Wisplinghoff H, Bischoff T, Tallent SM, Seifert H, Wenzel RP, et al. (2004) Nosocomial bloodstream infections in US hospitals: analysis of 24,179 cases from a prospective nationwide surveillance study. Clin Infect Dis 39: 309-317. (Pubitemid 39050477)
31. Sievert DM, Ricks P, Edwards JR, Schneider A, Patel J, et al. (2013) Antimicrobial-resistant pathogens associated with healthcare-associated infections: summary of data reported to the National Healthcare Safety Network at the Centers for Disease Control and Prevention, 2009-2010. Infect Control Hosp Epidemiol 34: 1-14.
32. Nagao M (2012) A multicentre analysis of epidemiology of the nosocomial bloodstream infections in Japanese university hospitals. Clin Microbiol Infect: Article first published online.
33. La Scola B, Raoult D (2009) Direct identification of bacteria in positive blood culture bottles by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry. PLoS One 4: e8041.