Application of an LC-MS/MS method for the simultaneous quantification of human intestinal transporter proteins absolute abundance using a QconCAT technique

Journal of Pharmaceutical and Biomedical Analysis

Volumn 110, Issue , 2015, Pages 27-33

  Harwood, M D ^a,b   Achour, B ^c   Russell, M R ^a,c   Carlson, G L ^a   Warhurst, G ^a   Rostami Hodjegan, A ^b,c  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

^b CERTARA UK LIMITED   (United Kingdom)

^c UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

Absolute abundance; Human intestine; QconCAT; Quantitative targeted absolute proteomics; Transporter proteins

Indexed keywords

ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); ALKALINE PHOSPHATASE; BREAST CANCER RESISTANCE PROTEIN; CYTOCHROME P450 3A4; CYTOCHROME P450 3A5; HUMAN PEPTIDE TRANSPORTER 1; MULTIDRUG RESISTANCE ASSOCIATED PROTEIN 2; ORGANIC SOLUTE TRANSPORTER SUBUNIT ALPHA; PROTEIN; UNCLASSIFIED DRUG; CARRIER PROTEIN;

AMINO ACID SEQUENCE; ANALYTIC METHOD; ARTICLE; BASEMENT MEMBRANE; CELL MEMBRANE; CHELATION; CONTROLLED STUDY; DRUG ABSORPTION; ENZYME ACTIVITY; HUMAN; HUMAN TISSUE; ILEUM; IN VITRO STUDY; INTESTINE CELL; JEJUNUM; LIQUID CHROMATOGRAPHY; PRIORITY JOURNAL; PROTEIN CONTENT; PROTEIN DEGRADATION; QUALITY CONTROL; QUANTITATIVE CONCATENATION; STANDARD; TANDEM MASS SPECTROMETRY; CALIBRATION; CHEMISTRY; CYTOLOGY; PROCEDURES; PROTEOMICS; REPRODUCIBILITY; STANDARDS; STATISTICAL MODEL; VALIDATION STUDY;

CALIBRATION; CELL MEMBRANE; CHROMATOGRAPHY, LIQUID; ENTEROCYTES; HUMANS; ILEUM; JEJUNUM; LINEAR MODELS; MEMBRANE TRANSPORT PROTEINS; PROTEOMICS; REFERENCE STANDARDS; REPRODUCIBILITY OF RESULTS; TANDEM MASS SPECTROMETRY;

EID: 84924989739     PISSN: 07317085     EISSN: 1873264X     Source Type: Journal    
DOI: 10.1016/j.jpba.2015.02.043     Document Type: Article

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