메뉴 건너뛰기




Volumn 25, Issue 3, 2015, Pages 205-214

Quantification of arbuscular mycorrhizal fungal DNA in roots: how important is material preservation?

Author keywords

Arbuscular mycorrhizal fungi; Intraradical colonization; PCR inhibition; Quantitative real time PCR (qPCR); Sample preservation

Indexed keywords

ARBUSCULAR MYCORRHIZA; DNA; FUNGUS; INHIBITION; LEGUME; POLYMERASE CHAIN REACTION; PRESERVATION; REAL TIME; ROOT COLONIZATION; ROOT SYSTEM;

EID: 84924962530     PISSN: 09406360     EISSN: 14321890     Source Type: Journal    
DOI: 10.1007/s00572-014-0602-7     Document Type: Article
Times cited : (16)

References (42)
  • 1
    • 54549094863 scopus 로고    scopus 로고
    • The impact of tillage practices on arbuscular mycorrhizal fungal diversity in subtropical crops
    • COI: 1:STN:280:DC%2BD1czis1ahsg%3D%3D, PID: 18488613
    • Alguacil MM, Lumini E, Roldan A, Salinas-García JR, Bonfante P, Bianciotto V (2008) The impact of tillage practices on arbuscular mycorrhizal fungal diversity in subtropical crops. Ecol Appl 18:527–536. doi:10.1890/07-0521.1.
    • (2008) Ecol Appl , vol.18 , pp. 527-536
    • Alguacil, M.M.1    Lumini, E.2    Roldan, A.3    Salinas-García, J.R.4    Bonfante, P.5    Bianciotto, V.6
  • 2
    • 1042278600 scopus 로고    scopus 로고
    • Quantification of the arbuscular mycorrhizal fungus Glomus intraradices in host tissue using real-time polymerase chain reaction
    • COI: 1:CAS:528:DC%2BD2cXhvFehtr0%3D
    • Alkan N, Gadkar V, Coburn J, Yarden O, Kapulnik Y (2004) Quantification of the arbuscular mycorrhizal fungus Glomus intraradices in host tissue using real-time polymerase chain reaction. New Phytol 161:877–885. doi:10.1111/j.1469-8137.2003.00975.x.
    • (2004) New Phytol , vol.161 , pp. 877-885
    • Alkan, N.1    Gadkar, V.2    Coburn, J.3    Yarden, O.4    Kapulnik, Y.5
  • 3
    • 33745146508 scopus 로고    scopus 로고
    • Analysis of quantitative interactions between two species of arbuscular mycorrhizal fungi, Glomus mosseae and G. intraradices, by real-time PCR
    • COI: 1:CAS:528:DC%2BD28XmtV2gur4%3D, PID: 16751531
    • Alkan N, Gadkar V, Yarden O, Kapulnik Y (2006) Analysis of quantitative interactions between two species of arbuscular mycorrhizal fungi, Glomus mosseae and G. intraradices, by real-time PCR. Appl Environ Microbiol 72:4192–4199. doi:10.1128/aem.02889-05.
    • (2006) Appl Environ Microbiol , vol.72 , pp. 4192-4199
    • Alkan, N.1    Gadkar, V.2    Yarden, O.3    Kapulnik, Y.4
  • 4
    • 77954083670 scopus 로고    scopus 로고
    • Differential effect of sample preservation methods on plant and arbuscular mycorrhizal fungal DNA
    • COI: 1:CAS:528:DC%2BC3cXosVGnu74%3D, PID: 20470836
    • Bainard LD, Klironomos JN, Hart MM (2010) Differential effect of sample preservation methods on plant and arbuscular mycorrhizal fungal DNA. J Microbiol Methods 82:124–130. doi:10.1016/j.mimet.2010.05.001.
    • (2010) J Microbiol Methods , vol.82 , pp. 124-130
    • Bainard, L.D.1    Klironomos, J.N.2    Hart, M.M.3
  • 6
    • 52649127918 scopus 로고    scopus 로고
    • Preferential colonization of Solanum tuberosum L. roots by the fungus Glomus intraradices in arable soil of a potato farming area
    • COI: 1:CAS:528:DC%2BD1cXhtFCqt7jL, PID: 18676711
    • Cesaro P, van Tuinen D, Copetta A, Chatagnier O, Berta G, Gianinazzi S, Lingua G (2008) Preferential colonization of Solanum tuberosum L. roots by the fungus Glomus intraradices in arable soil of a potato farming area. Appl Environ Microbiol 74:5776–5783. doi:10.1128/aem.00719-08.
    • (2008) Appl Environ Microbiol , vol.74 , pp. 5776-5783
    • Cesaro, P.1    van Tuinen, D.2    Copetta, A.3    Chatagnier, O.4    Berta, G.5    Gianinazzi, S.6    Lingua, G.7
  • 7
    • 0028978636 scopus 로고
    • Diversity of fungal symbionts in arbuscular mycorrhizas from a natural community
    • Clapp JP, Young JPW, Merryweather JW, Fitter AH (1995) Diversity of fungal symbionts in arbuscular mycorrhizas from a natural community. New Phytol 130:259–265. doi:10.1111/j.1469-8137.1995.tb03047.x.
    • (1995) New Phytol , vol.130 , pp. 259-265
    • Clapp, J.P.1    Young, J.P.W.2    Merryweather, J.W.3    Fitter, A.H.4
  • 8
    • 80052635554 scopus 로고    scopus 로고
    • Arbuscular mycorrhizal fungal communities in plant roots are not random assemblages
    • COI: 1:CAS:528:DC%2BC3MXht1CitLvE, PID: 21457278
    • Davison J, Opik M, Daniell TJ, Moora M, Zobel M (2011) Arbuscular mycorrhizal fungal communities in plant roots are not random assemblages. FEMS Microbiol Ecol 78:103–115. doi:10.1111/j.1574-6941.2011.01103.x.
    • (2011) FEMS Microbiol Ecol , vol.78 , pp. 103-115
    • Davison, J.1    Opik, M.2    Daniell, T.J.3    Moora, M.4    Zobel, M.5
  • 9
    • 22144470871 scopus 로고    scopus 로고
    • Contrasting root associated fungi of three common oak-woodland plant species based on molecular identification: host specificity or non-specific amplification?
    • COI: 1:CAS:528:DC%2BD2MXlvV2qtL8%3D, PID: 15772816
    • Douhan GW, Petersen C, Bledsoe CS, Rizzo DM (2005) Contrasting root associated fungi of three common oak-woodland plant species based on molecular identification: host specificity or non-specific amplification? Mycorrhiza 15:365–372. doi:10.1007/s00572-004-0341-2.
    • (2005) Mycorrhiza , vol.15 , pp. 365-372
    • Douhan, G.W.1    Petersen, C.2    Bledsoe, C.S.3    Rizzo, D.M.4
  • 11
    • 0344211422 scopus 로고    scopus 로고
    • Direct quantification of fungal DNA from soil substrate using real-time PCR
    • COI: 1:CAS:528:DC%2BD3sXhsVCmtLw%3D, PID: 12609725
    • Filion M, St-Arnaud M, Jabaji-Hare SH (2003) Direct quantification of fungal DNA from soil substrate using real-time PCR. J Microbiol Methods 53:67–76. doi:10.1016/S0167-7012(02)00225-7.
    • (2003) J Microbiol Methods , vol.53 , pp. 67-76
    • Filion, M.1    St-Arnaud, M.2    Jabaji-Hare, S.H.3
  • 12
    • 41649095297 scopus 로고    scopus 로고
    • Real-time PCR and microscopy: Are the two methods measuring the same unit of arbuscular mycorrhizal fungal abundance?
    • COI: 1:CAS:528:DC%2BD1cXks12ksrY%3D, PID: 17964831
    • Gamper HA, Young JPW, Jones DL, Hodge A (2008) Real-time PCR and microscopy: are the two methods measuring the same unit of arbuscular mycorrhizal fungal abundance? Fungal Genet Biol 45:581–596. doi:10.1016/j.fgb.2007.09.007.
    • (2008) Fungal Genet Biol , vol.45 , pp. 581-596
    • Gamper, H.A.1    Young, J.P.W.2    Jones, D.L.3    Hodge, A.4
  • 13
    • 2442484689 scopus 로고    scopus 로고
    • Diversity of arbuscular mycorrhizal fungi colonising roots of the grass species Agrostis capillaris and Lolium perenne in a field experiment
    • PID: 12768382
    • Gollotte A, van Tuinen D, Atkinson D (2004) Diversity of arbuscular mycorrhizal fungi colonising roots of the grass species Agrostis capillaris and Lolium perenne in a field experiment. Mycorrhiza 14:111–117. doi:10.1007/s00572-003-0244-7.
    • (2004) Mycorrhiza , vol.14 , pp. 111-117
    • Gollotte, A.1    van Tuinen, D.2    Atkinson, D.3
  • 14
    • 84986992409 scopus 로고
    • The effect of magnesium ions on the vesicular arbuscular mycorrhizal infection of maize roots
    • COI: 1:CAS:528:DyaK3sXitlaqtbg%3D
    • Gryndler M, Vejsadová H, Vančura V (1992) The effect of magnesium ions on the vesicular arbuscular mycorrhizal infection of maize roots. New Phytol 122:455–460. doi:10.1111/j.1469-8137.1992.tb00073.x.
    • (1992) New Phytol , vol.122 , pp. 455-460
    • Gryndler, M.1    Vejsadová, H.2    Vančura, V.3
  • 16
    • 0036223677 scopus 로고    scopus 로고
    • Selectivity and functional diversity in arbuscular mycorrhizas of co-occurring fungi and plants from a temperate deciduous woodland
    • Helgason T, Merryweather JW, Denison J, Wilson P, Young JPW, Fitter AH (2002) Selectivity and functional diversity in arbuscular mycorrhizas of co-occurring fungi and plants from a temperate deciduous woodland. J Ecol 90:371–384. doi:10.1046/j.1365-2745.2001.00674.x.
    • (2002) J Ecol , vol.90 , pp. 371-384
    • Helgason, T.1    Merryweather, J.W.2    Denison, J.3    Wilson, P.4    Young, J.P.W.5    Fitter, A.H.6
  • 17
    • 11444271007 scopus 로고    scopus 로고
    • Rapid determination of fungal colonization and arbuscule formation in roots of Medicago truncatula using real-time (RT) PCR
    • COI: 1:CAS:528:DC%2BD2MXhsVCjs7w%3D, PID: 15658808
    • Isayenkov S, Fester T, Hause B (2004) Rapid determination of fungal colonization and arbuscule formation in roots of Medicago truncatula using real-time (RT) PCR. J Plant Physiol 161:1379–1383. doi:10.1016/j.jplph.2004.04.012.
    • (2004) J Plant Physiol , vol.161 , pp. 1379-1383
    • Isayenkov, S.1    Fester, T.2    Hause, B.3
  • 18
    • 84885037700 scopus 로고    scopus 로고
    • Effects of inoculum additions in the presence of a preestablished arbuscular mycorrhizal fungal community
    • PID: 23956395
    • Janoušková M, Krak K, Wagg C, Štorchová H, Caklová P, Vosátka M (2013) Effects of inoculum additions in the presence of a preestablished arbuscular mycorrhizal fungal community. Appl Environ Microbiol 79:6507–6515. doi:10.1128/aem.02135-13.
    • (2013) Appl Environ Microbiol , vol.79 , pp. 6507-6515
    • Janoušková, M.1    Krak, K.2    Wagg, C.3    Štorchová, H.4    Caklová, P.5    Vosátka, M.6
  • 19
    • 38149052993 scopus 로고    scopus 로고
    • Are there benefits of simultaneous root colonization by different arbuscular mycorrhizal fungi?
    • COI: 1:CAS:528:DC%2BD1cXis1alt7s%3D, PID: 18042204
    • Jansa J, Smith FA, Smith SE (2008) Are there benefits of simultaneous root colonization by different arbuscular mycorrhizal fungi? New Phytol 177:779–789. doi:10.1111/j.1469-8137.2007.02294.x.
    • (2008) New Phytol , vol.177 , pp. 779-789
    • Jansa, J.1    Smith, F.A.2    Smith, S.E.3
  • 20
    • 84898546528 scopus 로고    scopus 로고
    • Soil and geography are more important determinants of indigenous arbuscular mycorrhizal communities than management practices in Swiss agricultural soils
    • COI: 1:CAS:528:DC%2BC2cXmtVegu7w%3D, PID: 24611988
    • Jansa J, Erb A, Oberholzer H-R, Šmilauer P, Egli S (2014) Soil and geography are more important determinants of indigenous arbuscular mycorrhizal communities than management practices in Swiss agricultural soils. Mol Ecol 23:2118–2135.
    • (2014) Mol Ecol , vol.23 , pp. 2118-2135
    • Jansa, J.1    Erb, A.2    Oberholzer, H.-R.3    Šmilauer, P.4    Egli, S.5
  • 23
    • 77953635682 scopus 로고    scopus 로고
    • TaqMan real-time PCR assays to assess arbuscular mycorrhizal responses to field manipulation of grassland biodiversity: effects of soil characteristics, plant species richness, and functional traits
    • PID: 20418424
    • König S, Wubet T, Dormann CF, Hempel S, Renker C, Buscot F (2010) TaqMan real-time PCR assays to assess arbuscular mycorrhizal responses to field manipulation of grassland biodiversity: effects of soil characteristics, plant species richness, and functional traits. Appl Environ Microbiol 76:3765–3775. doi:10.1128/aem.02951-09.
    • (2010) Appl Environ Microbiol , vol.76 , pp. 3765-3775
    • König, S.1    Wubet, T.2    Dormann, C.F.3    Hempel, S.4    Renker, C.5    Buscot, F.6
  • 24
    • 84879228334 scopus 로고
    • A modified procedure for staining roots to detect VA mycorrhizas
    • Koske RE, Gemma JN (1989) A modified procedure for staining roots to detect VA mycorrhizas. Mycol Res 92:486–505. doi:10.1016/S0953-7562(89)80195-9.
    • (1989) Mycol Res , vol.92 , pp. 486-505
    • Koske, R.E.1    Gemma, J.N.2
  • 25
    • 84861149131 scopus 로고    scopus 로고
    • Intraradical dynamics of two coexisting isolates of the arbuscular mycorrhizal fungus Glomus intraradices sensu lato as estimated by real-time PCR of mitochondrial DNA
    • COI: 1:CAS:528:DC%2BC38XmslWrs7o%3D, PID: 22407684
    • Krak K, Janoušková M, Caklová P, Vosátka M, Štorchová H (2012) Intraradical dynamics of two coexisting isolates of the arbuscular mycorrhizal fungus Glomus intraradices sensu lato as estimated by real-time PCR of mitochondrial DNA. Appl Environ Microbiol 78:3630–3637. doi:10.1128/aem.00035-12.
    • (2012) Appl Environ Microbiol , vol.78 , pp. 3630-3637
    • Krak, K.1    Janoušková, M.2    Caklová, P.3    Vosátka, M.4    Štorchová, H.5
  • 26
    • 47249139498 scopus 로고    scopus 로고
    • Quantification of Fusarium solani f. sp. glycines isolates in soybean roots by colony-forming unit assays and real-time quantitative PCR
    • COI: 1:CAS:528:DC%2BD1cXosVyjs74%3D, PID: 18461301
    • Li S, Hartman GL, Domier LL, Boykin D (2008) Quantification of Fusarium solani f. sp. glycines isolates in soybean roots by colony-forming unit assays and real-time quantitative PCR. Theor Appl Genet 117:343–352. doi:10.1007/s00122-008-0779-2.
    • (2008) Theor Appl Genet , vol.117 , pp. 343-352
    • Li, S.1    Hartman, G.L.2    Domier, L.L.3    Boykin, D.4
  • 27
    • 0025659986 scopus 로고
    • A new method which gives an objective measure of colonization of roots by vesicular arbuscular mycorrhizal fungi
    • McGonigle TP, Miller MH, Evans DG, Fairchild GL, Swan JA (1990) A new method which gives an objective measure of colonization of roots by vesicular arbuscular mycorrhizal fungi. New Phytol 115:495–501. doi:10.1111/j.1469-8137.1990.tb00476.x.
    • (1990) New Phytol , vol.115 , pp. 495-501
    • McGonigle, T.P.1    Miller, M.H.2    Evans, D.G.3    Fairchild, G.L.4    Swan, J.A.5
  • 28
    • 50649096399 scopus 로고    scopus 로고
    • Conventional PCR and real time quantitative PCR detection of Phytophthora cryptogea on Gerbera jamesonii
    • COI: 1:CAS:528:DC%2BD1cXhtVektL%2FM
    • Minerdi D, Moretti M, Li Y, Gaggero L, Garibaldi A, Gullino ML (2008) Conventional PCR and real time quantitative PCR detection of Phytophthora cryptogea on Gerbera jamesonii. Eur J Plant Pathol 122:227–237. doi:10.1007/s10658-008-9276-3.
    • (2008) Eur J Plant Pathol , vol.122 , pp. 227-237
    • Minerdi, D.1    Moretti, M.2    Li, Y.3    Gaggero, L.4    Garibaldi, A.5    Gullino, M.L.6
  • 29
    • 0345526437 scopus 로고    scopus 로고
    • Divergent arbuscular mycorrhizal fungal communities colonize roots of Pulsatilla spp. in boreal Scots pine forest and grassland soils
    • Öpik M, Moora M, Liira J, Koljalg U, Zobel M, Sen R (2003) Divergent arbuscular mycorrhizal fungal communities colonize roots of Pulsatilla spp. in boreal Scots pine forest and grassland soils. New Phytol 160:581–593. doi:10.1046/j.1469-8137x.2003.00917.x.
    • (2003) New Phytol , vol.160 , pp. 581-593
    • Öpik, M.1    Moora, M.2    Liira, J.3    Koljalg, U.4    Zobel, M.5    Sen, R.6
  • 30
    • 70349525156 scopus 로고    scopus 로고
    • Large-scale parallel 454 sequencing reveals host ecological group specificity of arbuscular mycorrhizal fungi in a boreonemoral forest
    • PID: 19558424
    • Öpik M, Metsis M, Daniell TJ, Zobel M, Moora M (2009) Large-scale parallel 454 sequencing reveals host ecological group specificity of arbuscular mycorrhizal fungi in a boreonemoral forest. New Phytol 184:424–437. doi:10.1111/j.1469-8137.2009.02920.x.
    • (2009) New Phytol , vol.184 , pp. 424-437
    • Öpik, M.1    Metsis, M.2    Daniell, T.J.3    Zobel, M.4    Moora, M.5
  • 31
    • 77956292194 scopus 로고    scopus 로고
    • The online database MaarjAM reveals global and ecosystemic distribution patterns in arbuscular mycorrhizal fungi (Glomeromycota)
    • PID: 20561207
    • Öpik M, Vanatoa A, Vanatoa E, Moora M, Davison J, Kalwij JM, Reier Ü, Zobel M (2010) The online database MaarjAM reveals global and ecosystemic distribution patterns in arbuscular mycorrhizal fungi (Glomeromycota). New Phytol 188:223–241. doi:10.1111/j.1469-8137.2010.03334.x.
    • (2010) New Phytol , vol.188 , pp. 223-241
    • Öpik, M.1    Vanatoa, A.2    Vanatoa, E.3    Moora, M.4    Davison, J.5    Kalwij, J.M.6    Reier, $1.7    Zobel, M.8
  • 32
    • 0029751313 scopus 로고    scopus 로고
    • The genetic diversity of arbuscular mycorrhizal fungi in natural ecosystems—a key to understanding the ecology and functioning of the mycorrhizal symbiosis
    • Sanders IR, Clapp JP, Wiemken A (1996) The genetic diversity of arbuscular mycorrhizal fungi in natural ecosystems—a key to understanding the ecology and functioning of the mycorrhizal symbiosis. New Phytol 133:123–134. doi:10.1111/j.1469-8137.1996.tb04348.x.
    • (1996) New Phytol , vol.133 , pp. 123-134
    • Sanders, I.R.1    Clapp, J.P.2    Wiemken, A.3
  • 33
    • 84889670117 scopus 로고    scopus 로고
    • Use of quantitative PCR detection methods to study biocontrol agents and phytopathogenic fungi and oomycetes in environmental samples
    • COI: 1:CAS:528:DC%2BC3sXhvV2lsL%2FL
    • Sanzani SM, Nicosia M, Faedda R, Cacciola SO, Schena L (2014) Use of quantitative PCR detection methods to study biocontrol agents and phytopathogenic fungi and oomycetes in environmental samples. J Phytopathol 162:1–13. doi:10.1111/jph.12147.
    • (2014) J Phytopathol , vol.162 , pp. 1-13
    • Sanzani, S.M.1    Nicosia, M.2    Faedda, R.3    Cacciola, S.O.4    Schena, L.5
  • 34
    • 84876131610 scopus 로고    scopus 로고
    • Development of quantitative PCR detection methods for phytopathogenic fungi and oomycetes
    • COI: 1:CAS:528:DC%2BC2cXhvVKmtLzP
    • Schena L, Nicosia M, Sanzani SM, Faedda R, Ippolito A, Cacciola SO (2013) Development of quantitative PCR detection methods for phytopathogenic fungi and oomycetes. J Plant Pathol 95:7–24. doi:10.4454/JPP.V95I1.016.
    • (2013) J Plant Pathol , vol.95 , pp. 7-24
    • Schena, L.1    Nicosia, M.2    Sanzani, S.M.3    Faedda, R.4    Ippolito, A.5    Cacciola, S.O.6
  • 37
    • 84856761198 scopus 로고    scopus 로고
    • Real-time PCR to quantify composition of arbuscular mycorrhizal fungal communities—marker design, verification, calibration and field validation
    • COI: 1:CAS:528:DC%2BC38XkvVCmt78%3D, PID: 22059700
    • Thonar C, Erb A, Jansa J (2012) Real-time PCR to quantify composition of arbuscular mycorrhizal fungal communities—marker design, verification, calibration and field validation. Mol Ecol Resour 12(2):219–232. doi:10.1111/j.1755-0998.2011.03086.x.
    • (2012) Mol Ecol Resour , vol.12 , Issue.2 , pp. 219-232
    • Thonar, C.1    Erb, A.2    Jansa, J.3
  • 38
    • 84893010959 scopus 로고    scopus 로고
    • Competition and facilitation in synthetic communities of arbuscular mycorrhizal fungi
    • PID: 24330316
    • Thonar C, Frossard E, Šmilauer P, Jansa J (2014) Competition and facilitation in synthetic communities of arbuscular mycorrhizal fungi. Mol Ecol 23:733–746. doi:10.1111/mec.12625.
    • (2014) Mol Ecol , vol.23 , pp. 733-746
    • Thonar, C.1    Frossard, E.2    Šmilauer, P.3    Jansa, J.4
  • 39
    • 0000061180 scopus 로고
    • In planta histochemical staining of fungal alkaline phosphatase activity for analysis of efficient arbuscular mycorrhizal infections
    • COI: 1:CAS:528:DyaK3sXkvFWnt70%3D
    • Tisserant B, Gianinazzi-Pearson V, Gianinazzi S, Gollotte A (1993) In planta histochemical staining of fungal alkaline phosphatase activity for analysis of efficient arbuscular mycorrhizal infections. Mycol Res 97:245–250. doi:10.1016/S0953-7562(09)80248-7.
    • (1993) Mycol Res , vol.97 , pp. 245-250
    • Tisserant, B.1    Gianinazzi-Pearson, V.2    Gianinazzi, S.3    Gollotte, A.4
  • 41
    • 28444459983 scopus 로고    scopus 로고
    • An overview of methods for the detection and observation of arbuscular mycorrhizal fungi in roots
    • COI: 1:CAS:528:DC%2BD2MXhtlWms7bO
    • Vierheilig H, Schweiger P, Brundrett M (2005) An overview of methods for the detection and observation of arbuscular mycorrhizal fungi in roots. Physiol Plant 125:393–404. doi:10.1111/j.1399-3054.2005.00564.x.
    • (2005) Physiol Plant , vol.125 , pp. 393-404
    • Vierheilig, H.1    Schweiger, P.2    Brundrett, M.3
  • 42
    • 79959897816 scopus 로고    scopus 로고
    • Mycorrhizal fungal identity and diversity relaxes plant-plant competition
    • PID: 21797158
    • Wagg C, Jansa J, Stadler M, Schmid B, van der Heijden MGA (2011) Mycorrhizal fungal identity and diversity relaxes plant-plant competition. Ecology 92:1303–1313. doi:10.1890/10-1915.1.
    • (2011) Ecology , vol.92 , pp. 1303-1313
    • Wagg, C.1    Jansa, J.2    Stadler, M.3    Schmid, B.4    van der Heijden, M.G.A.5


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.