Successful detection of pathogenic Shiga-toxin-producing Escherichia coli in shellfish, environmental waters and sediment using the ISO/TS-13136 method

Letters in Applied Microbiology

Volumn 60, Issue 4, 2015, Pages 315-320

  Baliere C ^a   Rince A ^b   Thevenot, D ^c   Gourmelon, M ^a  

^a IFREMER   (France)

^b UNIVERSITÉ DE CAEN   (France)

^c University of Lyon   (France)

Author keywords

stx; eae; Sediment; Shellfish; Shiga toxin producing Escherichia coli; Water

Indexed keywords

ESCHERICHIA COLI; GENES; HEALTH RISKS; MOLLUSCS; POLYMERASE CHAIN REACTION; SHELLFISH; TOXIC MATERIALS;

EAE; ENVIRONMENTAL WATER; PATHOGENICS; PCR DETECTION; REAL-TIME PCR; SEDIMENT SAMPLES; SHIGA TOXIN-PRODUCING ESCHERICHIA COLI; STX; STX GENES; WATER SAMPLES;

SEDIMENTS;

BACTERIUM; COASTAL ZONE; GENE EXPRESSION; GENETIC MARKER; HEALTH RISK; PATHOGEN; POLYMERASE CHAIN REACTION; PUBLIC HEALTH; REAL TIME; SHELLFISH; TOXIN;

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM DETECTION; COASTAL WATERS; COCKLE; EAE GENE; ESCHERICHIA COLI INFECTION; GENETIC MARKER; MUSSEL; NONHUMAN; OYSTER; REAL TIME POLYMERASE CHAIN REACTION; SEDIMENT; SHELLFISH; SHIGA TOXIN PRODUCING ESCHERICHIA COLI; STX1 GENE; STX2 GENE; WATER CONTAMINATION; ANIMAL; BIOSYNTHESIS; CLASSIFICATION; ESCHERICHIA COLI INFECTIONS; GENETICS; HUMAN; ISOLATION AND PURIFICATION; MICROBIOLOGY; MOLLUSC;

ESCHERICHIA COLI; OSTREIDAE;

ADHESIN; EAEA PROTEIN, E COLI; ESCHERICHIA COLI PROTEIN; GENETIC MARKER; SHIGA TOXIN; VEROTOXIN 1; VEROTOXIN 2;

ADHESINS, BACTERIAL; ANIMALS; ESCHERICHIA COLI INFECTIONS; ESCHERICHIA COLI PROTEINS; GENETIC MARKERS; GEOLOGIC SEDIMENTS; HUMANS; MOLLUSCA; REAL-TIME POLYMERASE CHAIN REACTION; SHELLFISH; SHIGA TOXIN; SHIGA TOXIN 1; SHIGA TOXIN 2; SHIGA-TOXIGENIC ESCHERICHIA COLI;

EID: 84924715277     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/lam.12386     Document Type: Article

References (30)

1. Anon. (1998) ISO 9308-3 Water quality - Detection and enumeration of Escherichia coli and coliform bacteria in surface and waste water - Part 3: Miniaturized method (Most Probable Number) by inoculation in liquid medium. ISO, 20 p.
2. Anon. (2000) NF V 08-600 Microbiologie des aliments - Dénombrement des Escherichia coli présumés dans les coquillages vivants - Technique du nombre le plus propable (Microbiologie of Foods and Foodstuffs Products - Enumeration of Presumptive Escherichia coli in Living Shellfish - MPN Technique). AFNOR, 16 p.
3. Anonymous. (2004) Regulation (EC) No 854/2004 of the European parliament and of the council of 29 April 2004 laying down specific rules for the organisation of official controls on products of animal origin intended for human consumption.
4. Auvray, F., Lecureuil, C., Taché, J., Leclerc, V., Deperrois, V. and Lombard, B. (2007) Detection, isolation and characterization of Shiga toxin-producing Escherichia coli in retail-minced beef using PCR-based techniques, immunoassays and colony hybridization. Lett Appl Microbiol 45, 646-651.
5. Bennani, M., Badri, S., Baibai, T., Oubrim, N., Hassar, M., Cohen, N. and Amarouch, H. (2011) First detection of Shiga toxin-producing Escherichia coli in shellfish and coastal environments of Morocco. Appl Biochem Biotechnol 165, 290-299.
6. Beutin, L. and Fach, P. (2014) Detection of Shiga toxin-producing Escherichia coli from nonhuman sources and strain typing. Microbiol Spectr 2, EHEC-0001-2013.
7. Bibbal, D., Loukiadis, E., Kérourédan, M., Peytavin de Garam, C., Ferré, F., Cartier, P., Gay, E., Oswald, E. et al. (2014) Intimin gene (eae) subtype-based real-time PCR strategy for specific detection of Shiga toxin-producing Escherichia coli serotypes O157:H7, O26:H11, O103:H2, O111:H8, and O145:H28 in cattle feces. Appl Environ Microbiol 80, 1177-1184.
8. DGAL. Direction générale de l'alimentation. (2013) Plans de surveillance-Plan de contrôle, Bilan 2012. Ministère de l'agriculture, de l'agroalimentaire et de la fôret. Octobre.
9. EFSA. European Food Safety Authority. (2009) Technical specifications for the monitoring and reporting of verotoxigenic Escherichia coli (VTEC) on animals and food (VTEC surveys on animals and food). EFSA J.7:1366-1409.http://dx.doi.org/10.2903/j.efsa.2009.1366.
10. Feng, P., Dey, M., Abe, A. and Takeda, T. (2001) Isogenic strain of Escherichia coli O157:H7 that has lost both Shiga toxin 1 and 2 Genes. ASM 8, 711-717.
11. Gourmelon, M., Montet, M.P., Lozach, S., Le Mennec, C., Pommepuy, M., Beutin, L. and Vernozy-Rozand, C. (2006) First isolation of Shiga toxin 1d producing Escherichia coli variant strains in shellfish from coastal areas in France. J Appl Microbiol 100, 85-97.
12. Guyon, R., Dorey, F., Collobert, J.F., Foret, J., Goubert, C., Mariau, V. and Malas, J.P. (2000) Detection of shiga toxin-producing Escherichia coli O157 in shellfish (Crassostrea gigas). Sci Aliment 20, 457-466.
13. Gyles, C.L. (2007) Shiga toxin-producing Escherichia coli: an overview. J Anim Sci 85, 45-62.
14. ISO. (2012) ISO/TS-13136:2012: Microbiology of Food and Animal Feed. Real-Time Polymerase Chain Reaction (PCR)-Based Method for the Detection of Food-Borne Pathogens. Horizontal Method for the Detection of Shiga Toxin-Producing Escherichia coli (STEC) and the Determination of O157, O111, O26, O103 and O145 Serogroups. Geneva, Switzerland: International Organization for Standardization.
15. Loukiadis, E., Kérourédan, M., Beutin, L., Oswald, E. and Brugère, H. (2006) Characterization of Shiga toxin gene (stx)-positive and intimin gene (eae)-positive Escherichia coli isolates from wastewater of slaughterhouses in France. Appl Environ Microbiol 72, 3245-3251.
16. MacRae, M., Hamilton, C., Strachan, N.J., Wright, S. and Ogden, I.D. (2005) The detection of Cryptosporidium parvum and Escherichia coli O157 in UK bivalve shellfish. J Microbiol Methods 60, 395-401.
17. Madic, J., Vingadassalon, N., De Garam, C.P., Marault, M., Scheutz, F., Brugère, H., Jamet, E. and Auvray, F. (2011) Detection of Shiga toxin-producing Escherichia coli serotypes O26:H11, O103:H2, O111:H8, O145:H28, and O157:H7 in raw-milk cheeses by using multiplex real-time PCR. Appl Environ Microbiol 77, 2035-2041.
18. Martinez-Castillo, A., Quirós, P., Navarro, F., Miró, E. and Muniesa, M. (2013) Shiga toxin 2-encoding bacteriophages in human fecal samples from healthy individuals. Appl Environ Microbiol 79, 4862-4868.
19. Muniesa, M., Lucena, F. and Jofre, J. (1999) Study of the potential relationship between the morphology of infectious somatic coliphages and their persistence in the environment. J Appl Microbiol 87, 402-409.
20. Nielsen, E.M. and Andersen, M.T. (2003) Detection and characterization of Verocytotoxin-producing Escherichia coli by automated 5′ nuclease PCR Assay. J Clin Microbiol 41, 2884-2893.
21. O'Brien, A.D., Newland, J.W., Miller, S.F., Holmes, R.K., Smith, H.W. and Formal, S.B. (1984) Shiga-like toxin-converting phages from Escherichia coli strains that cause hemorrhagic colitis or infantile diarrhea. Science 226, 694-696.
22. Ogden, L.D., Fenlon, D.R., Vinten, A.J. and Lewis, D. (2001) The fate of Escherichia coli O157 in soil and its potential to contaminate drinking water. Int J Food Microbiol 66, 111-117.
23. Paton, J.C. and Paton, A.W. (1998) Pathogenesis and diagnosis of Shiga toxin-producing Escherichia coli infections. Clin Microbiol Rev 11, 450-479.
24. Perelle, S., Dilasser, F., Grout, J. and Fach, P. (2007) Screening food raw materials for the presence of the world's most frequent clinical cases of Shiga toxin-encoding Escherichia coli O26, O103, O111, O145 and O157. Int J Food Microbiol 113, 284-288.
25. Posse, B., De Zutter, L., Heyndrickx, M. and Herman, L. (2008) Novel differential and confirmation plating media for Shiga toxin-producing Escherichia coli serotypes O26, O103, O111, O145 and sorbitol-positive and -negative O157. FEMS Microbiol Lett 282, 124-131.
26. Potasman, I., Paz, A. and Odeh, M. (2002) Infectious outbreaks associated with bivalve shellfish consumption: a worldwide perspective. Clin Infect Dis 35, 921-928.
27. Pradel, N., Livrelli, V., Champs, C.D., Palcoux, J.-B., Reynaud, A., Scheutz, F., Sirot, J., Joly, B. et al. (2000) Prevalence and characterization of Shiga toxin-producing Escherichia coli isolated from cattle, food, and children during a one-year prospective study in France. J Clin Microbiol 38, 1023-1031.
28. Sanath Kumar, H., Otta, S.K. and Karunasagar, I. (2001) Detection of Shiga-toxigenic Escherichia coli (STEC) in fresh seafood and meat marketed in Mangalore, India by PCR. Lett Appl Microbiol 33, 334-338.
29. Vernozy-Rozand, C., Montet, M.P., Lequerrec, F., Serillon, E., Tilly, B., Bavai, C., Ray-Gueniot, S., Bouvet, J. et al. (2002) Prevalence of Verotoxin-producing Escherichia coli (VTEC) in slurry, farmyard manure and sewage sludge in France. J Appl Microbiol 93, 473-478.
30. Wetzel, A.N. and LeJeune, J.T. (2007) Isolation of Escherichia coli O157:H7 strains that do not produce Shiga toxin from bovine, avian and environmental sources. Lett Appl Microbiol 45, 504-507.