Isolation of Escherichia coli O157:H7 strains that do not produce Shiga toxin from bovine, avian and environmental sources

Letters in Applied Microbiology

Volumn 45, Issue 5, 2007, Pages 504-507

  Wetzel, A N ^a   LeJeune, J T ^a  

^a OHIO STATE UNIVERSITY   (United States)

Author keywords

Bacteriophage; Escherichia coli; Escherichia coli O157; Lysogeny; Shiga toxin 2

Indexed keywords

ESCHERICHIA COLI; FARMS; GENES; MAMMALS; TOXIC MATERIALS;

E. COLI O157; E.COLI O157; E.COLI O157:H7; ESCHERICHIA COLI O157; ESCHERICHIA COLI O157:H7; LYSOGENY; NATURALLY OCCURRING; SHIGA TOXIN; SHIGA TOXIN 2; STX GENES;

BACTERIOPHAGES;

SHIGA TOXIN;

ESCHERICHIA COLI; GENES; STRAIN; TOXICITY;

BACTERIUM; BIRD; CATTLE; DAIRY FARMING; TOXIN;

ARTICLE; BACTERIAL STRAIN; BACTERIOPHAGE; BACTERIUM ISOLATION; CONTROLLED STUDY; COW; ESCHERICHIA COLI; FOOD INDUSTRY; FOWL; LYSOGENIZATION; NONHUMAN;

NORTH AMERICA; OHIO; UNITED STATES;

ANIMALIA; AVES; BOS; BOVINAE; ESCHERICHIA COLI;

EID: 35448994834     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/j.1472-765X.2007.02228.x     Document Type: Article

References (19)

1. Barkocy-Gallagher, G.A., Arthur, T.M., Rivera-Betancourt, M., Nou, X., Shackelford, S.D., Wheeler, T.L. Koohmaraie, M. (2004) Characterization of O157:H7 and other Escherichia coli isolates recovered from cattle hides, feces, and carcasses. J Food Prot 67, 993 998.
2. Boerlin, P., McEwen, S.A., Boerlin-Petzold, F., Wilson, J.B., Johnson, R.P. Gyles, C.L. (1999) Associations between virulence factors of Shiga toxin-producing Escherichia coli and disease in humans. J Clin Microbiol 37, 497 503.
3. Bonardi, S., Maggi, E., Pizzin, G., Morabito, S. Caprioli, A. (2001) Faecal carriage of Verocytotoxin-producing Escherichia coli O157 and carcass contamination in cattle at slaughter in northern Italy. Int J Food Microbiol 66, 47 53.
4. CDC (2001). One-Day (24-28 h) Standardized Laboratory Protocol for Molecular Subtyping of Escherichia coli O157:H7 by Pulsed Field Gel Electrophoresis (PFGE). Atlanta, GA : CDC.
5. Davis, M.A., Hancock, D.D., Besser, T.E. Call, D.R. (2003) Evaluation of pulsed-field gel electrophoresis as a tool for determining the degree of genetic relatedness between strains of Escherichia coli O157:H7. J Clin Microbiol 41, 1843 1849.
6. Feng, P., Dey, M., Abe, A. Takeda, T. (2001) Isogenic strain of Escherichia coli O157:H7 that has lost both Shiga toxin 1 and 2 genes. Clin Diagn Lab Immunol 8, 711 717.
7. Gentry, M.K. Dalrymple, J.M. (1980) Quantitative microtiter cytotoxicity assay for Shigella toxin. J Clin Microbiol 12, 361 366.
8. Griffin, P.M. Tauxe, R.V. (1991) The epidemiology of infections caused by Escherichia coli O157:H7, other enterohemorrhagic E. coli, and the associated hemolytic uremic syndrome. Epidemiol Rev 13, 60 98.
9. Iguchi, A., Osawa, R., Kawano, J., Shimizu, A., Terajima, J. Watanabe, H. (2003) Effects of lysogeny of Shiga toxin 2-encoding bacteriophages on pulsed-field gel electrophoresis fragment pattern of Escherichia coli K-12. Curr Microbiol 46, 224 227.
10. Karch, H., Meyer, T., Russmann, H. Heesemann, J. (1992) Frequent loss of Shiga-like toxin genes in clinical isolates of Escherichia coli upon subcultivation. Infect Immun 60, 3464 3467.
11. LeJeune, J. Kauffman, M. (2005) Effect of sand and sawdust bedding material on the fecal prevalence of Escherichia coli O157:H7 in dairy cows. Appl Environ Microbiol 71, 326 330.
12. LeJeune, J.T., Abedon, S.T., Takemura, K., Christie, N.P. Sreevatsan, S. (2004) Human Escherichia coli O157:H7 genetic marker in isolates of bovine origin. Emerg Infect Dis 10, 1482 1485.
13. Muniesa, M., Garcia, A., Miro, E., Mirelis, B., Prats, G., Jofre, J. Navarro, F. (2004) Bacteriophages and diffusion of beta-lactamase genes. Emerg Infect Dis 10, 1134 1137.
14. Nielsen, E.M. Scheutz, F. (2002) Characterisation of Escherichia coli O157 isolates from Danish cattle and human patients by genotyping and presence and variants of virulence genes. Vet Microbiol 88, 259 273.
15. Schmidt, H., Bielaszewska, M. Karch, H. (1999a) Transduction of enteric Escherichia coli isolates with a derivative of Shiga toxin 2-encoding bacteriophage phi3538 isolated from Escherichia coli O157:H7. Appl Environ Microbiol 65, 3855 3861.
16. Schmidt, H., Scheef, J., Huppertz, H.I., Frosch, M. Karch, H. (1999b) Escherichia coli O157:H7 and O157:H(-) strains that do not produce Shiga toxin: phenotypic and genetic characterization of isolates associated with diarrhea and hemolytic-uremic syndrome. J Clin Microbiol 37, 3491 3496.
17. Smith, H.R., Day, N.P., Scotland, S.M., Gross, R.J. Rowe, B. (1984) Phage-determined production of vero cytotoxin in strains of Escherichia coli serogroup O157. Lancet 1, 1242 1243.
18. Wang, G., Clarke, C. Rodgers, F. (2002) Detection in Escherichia coli of the genes encoding the major virulence factors, the genes defining the O157:H7 serotype, and components of the type 2 shiga toxin family by mulitiplex PCR. J Clin Microbiol 40, 3613 3619.
19. Wetzel, A.N. LeJeune, J.T. (2006) Clonal dissemination of Escherichia coli O157:H7 subtypes among dairy farms in northeast Ohio. Appl Environ Microbiol 72, 2621 2626.