Reversibility of the Snail-induced epithelial-mesenchymal transition revealed by the Cre-loxP system

Biochemical and Biophysical Research Communications

Volumn 458, Issue 3, 2015, Pages 608-613

  Ozawa, Masayuki ^a   Kobayashi, Wakako ^a  

^a KAGOSHIMA UNIVERSITY   (Japan)

Author keywords

Cre loxP; EMT; MET; Reversibility; Snail

Indexed keywords

CRE RECOMBINASE; TRANSCRIPTION FACTOR SNAIL; UVOMORULIN; CADHERIN; INTEGRASE; SNAIL FAMILY TRANSCRIPTION FACTORS; TRANSCRIPTION FACTOR;

ANIMAL CELL; ARTICLE; CELL STRUCTURE; CONTROLLED STUDY; DOWN REGULATION; EPITHELIAL MESENCHYMAL TRANSITION; LOXP SITE; MDCK CELL LINE; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; ANIMAL; CELL LINE; DNA METHYLATION; DOG; ENTEROBACTERIA PHAGE P1; ENZYMOLOGY; GENE EXPRESSION; GENE VECTOR; GENETICS; HUMAN; TRANSGENE;

GASTROPODA;

ANIMALS; BACTERIOPHAGE P1; CADHERINS; CELL LINE; DNA METHYLATION; DOGS; EPITHELIAL-MESENCHYMAL TRANSITION; GENE EXPRESSION; GENETIC VECTORS; HUMANS; INTEGRASES; MADIN DARBY CANINE KIDNEY CELLS; PROMOTER REGIONS, GENETIC; TRANSCRIPTION FACTORS; TRANSGENES;

EID: 84924061226     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2015.02.012     Document Type: Article

References (33)

1. J.P. Thiery Epithelial-mesenchymal transitions in tumour progression Nat. Rev. Cancer 2 2002 442 454
2. R. Kalluri, and R.A. Weinberg The basics of epithelial-mesenchymal transition J. Clin. Invest. 119 2009 1420 1428
3. A. Cano, M.A. Pérez-Moreno, I. Rodrigo, and A. Locascio The transcription factor Snail controls epithelial-mesenchymal transitions by repressing E-cadherin expression Nat. Cell Biol. 2 2000 76 83
4. E. Batlle, E. Sancho, C. Francí, and D. Domínguez The transcription factor Snail is a repressor of E-cadherin gene expression in epithelial tumour cells Nat. Cell Biol. 2 2000 84 89
5. H. Peinado, E. Ballestar, M. Esteller, and A. Cano Snail mediates E-cadherin repression by the recruitment of the Sin3A/histone deacetylase 1 (HDAC1)/HDAC2 complex Mol. Cell. Biol. 24 2004 306 319
6. H. Cedar, and Y. Bergman Linking DNA methylation and histone modification: patterns and paradigms Nat. Rev. Genet. 10 2009 295 304
7. M.T. McCabe, J.C. Brandes, and P.M. Vertino Cancer DNA methylation: molecular mechanisms and clinical implications Clin. Cancer Res. 15 2009 3927 3937
8. H. Peinado, M. Quintanilla, and A. Cano Transforming growth factor β-1 induces snail transcription factor in epithelial cell lines: mechanisms for epithelial mesenchymal transitions J. Biol. Chem. 278 2003 21113 21123
9. L.A. Romano, and R.B. Runyan Slug is an essential target of TGFβ2 signaling in the developing chicken heart Dev. Biol. 223 2000 91 102
10. X. Yang, B. Pursell, S. Lu, and T.K. Chang Regulation of β4-integrin expression by epigenetic modifications in the mammary gland and during the epithelial-to-mesenchymal transition J. Cell Sci. 122 2009 2473 2480
11. C. Dong, Y. Wu, J. Yao, and Y. Wang G9a interacts with snail and is critical for snail-mediated E-cadherin repression in human breast cancer J. Clin. Invest. 122 2012 1469 1486
12. P.J. Miettinen, R. Ebner, A.R. Lopez, and R. Derynck TGF-beta induced transdifferentiation of mammary epithelial cells to mesenchymal cells: involvement of type I receptors J. Cell Biol. 127 1994 2021 2036
13. P.A. Gregory, C.P. Bracken, E. Smith, and A.G. Bert An autocrine TGF-beta/ZEB/miR-200 signaling network regulates establishment and maintenance of epithelial-mesenchymal transition Mol. Biol. Cell 22 2011 1686 1698
14. D. Olmeda, M. Jordá, H. Peinado, and A. Fabra Snail silencing effectively suppresses tumour growth and invasiveness Oncogene 26 2007 1862 1874
15. N. Sternberg, and D. Hamilton Bacteriophage P1 site specific recombination. I. Recombination between loxP sites J. Mol. Biol. 150 1981 467 486
16. B. Sauer, and N. Henderson Site-specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage P1 Proc. Natl. Acad. Sci. U.S.A. 85 1988 5166 5170
17. T. Ohkubo, and M. Ozawa The transcription factor Snail downregulates the tight junction components independently of E-cadherin downregulation J. Cell Sci. 117 2003 1675 1685
18. H. Niwa, K. Yamamura, and J. Miyazaki Efficient selection for high-expression transfectants with a novel eukaryotic vector Gene 108 1991 193 199
19. M. Sato, Y. Yasuoka, H. Kodama, and T. Watanabe New approach to cell lineage analysis in mammals using the Cre-loxP system Mol. Reprod. Dev. 56 2000 34 44
20. A. Nakayama, M. Sato, M. Shinohara, and S. Matsubara Efficient transfection of primarily cultured porcine embryonic fibroblasts using the Amaxa Nucleofection system Cloning Stem Cells 9 2007 523 534
21. B.G. Herrmann, and A.M. Frischauf Isolation of genomic DNA Methods Enzymol. 152 1987 180 183
22. M. Ozawa, and W. Kobayashi Cadherin cytoplasmic domains inhibit the cell surface localization of endogenous E-cadherin, blocking desmosome and tight junction formation and inducing cell dissociation PLoS One 9 2014 e105313
23. E.D. Hay, and A. Zuk Transformations between epithelium and mesenchyme: normal, pathological, and experimentally induced Am. J. Kidney Dis. 26 1995 678 690
24. J. Comijn, G. Berx, and P. Vermassen The two-handed E box binding zinc finger protein SIP1 downregulates E-cadherin and induces invasion Mol. Cell 7 2001 1267 1278
25. P. Zhang, Y. Wei, L. Wang, and B.G. Debeb ATM-mediated stabilization of ZEB1 promotes DNA damage response and radioresistance through CHK1 Nat. Cell Biol. 16 2014 864 875
26. F. Carrozzino, P. Soulié, D. Huber, and N. Mensi Inducible expression of Snail selectively increases paracellular ion permeability and differentially modulates tight junction proteins Am. J. Physiol. Cell Physiol. 289 2005 C1002 C1014
27. B. De Craene, B. Gilbert, C. Stove, and E. Bruyneel The transcription factor snail induces tumor cell invasion through modulation of the epithelial cell differentiation program Cancer Res. 2005 6237 6244
28. S.A. Mani, W. Guo, M.J. Liao, and E.N. Eaton The epithelial-mesenchymal transition generates cells with properties of stem cells Cell 133 2008 704 715
29. J.G. Lyons, V. Patel, N.C. Roue, and S.Y. Fok Snail up-regulates proinflammatory mediators and inhibits differentiation in oral keratinocytes Cancer Res. 68 2008 4525 4530
30. L.A. Ribeiro-Filho, J. Franks, M. Sasaki, and H. Shiina CpG hypermethylation of promoter region and inactivation of E-cadherin gene in human bladder cancer Mol. Carcinog. 34 2002 187 198
31. C.W. Cheng, P.E. Wu, J.C. Yu, and C.S. Huang Mechanisms of inactivation of E-cadherin in breast carcinoma: modification of the two-hit hypothesis of tumor suppressor gene Oncogene 20 2001 3814 3823
32. H. Peinado, F. Portillo, and A. Cano Transcriptional regulation of cadherins during development and carcinogenesis Int. J. Dev. Biol. 48 2004 365 375
33. T. Shirakihara, M. Saitoh, and K. Miyazono Differential regulation of epithelial and mesenchymal markers by deltaEF1 proteins in epithelial mesenchymal transition induced by TGF-beta Mol. Biol. Cell 18 2007 3533 3544