Analysis of acetohydroxyacid synthase variants from branched-chain amino acids-producing strains and their effects on the synthesis of branched-chain amino acids in Corynebacterium glutamicum

Protein Expression and Purification

Volumn 109, Issue , 2015, Pages 106-112

  Guo, Yanfeng ^a   Han, Mei ^a,b   Xu, Jianzhong ^a   Zhang, Weiguo ^a  

^a JIANGNAN UNIVERSITY   (China)

^b State Key Laboratory of Dairy Biotechnology Technology Center Bright Dairy and Food Co Ltd   (China)

Author keywords

AHAS; BCAAs; Catalytic subunit; Regulatory subunit; Resistance; Variant

Indexed keywords

CORYNEBACTERIUM GLUTAMICUM;

ACETOLACTATE SYNTHASE; BRANCHED CHAIN AMINO ACID; MUTANT PROTEIN;

BIOSYNTHESIS; CORYNEBACTERIUM GLUTAMICUM; ENZYMOLOGY; METABOLISM; POLYACRYLAMIDE GEL ELECTROPHORESIS; SEQUENCE HOMOLOGY;

ACETOLACTATE SYNTHASE; AMINO ACIDS, BRANCHED-CHAIN; CORYNEBACTERIUM GLUTAMICUM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; MUTANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 84923785336     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2015.02.006     Document Type: Article

References (26)

1. D.T. Le, M.Y. Yoon, and Y.T. Kim Homology modeling of the structure of tobacco acetohydroxy acid synthase and examination of the active site by site-directed mutagenesis Biochem. Biophys. Res. Commun. 317 2004 930 938
2. D.M. Chipman, R.G. Duggleby, and K. Tittmann Mechanisms of acetohydroxyacid synthases Curr. Opin. Chem. Biol. 9 2005 475 481
3. D. Leyval, D. Uy, S. Delaunay, J.L. Goergen, and J.M. Engasser Characterisation of the enzyme activities involved in the valine biosynthetic pathway in a valine-producing strain of Corynebacterium glutamicum J. Biotechnol. 104 2003 241 252
4. P.C. Calder Branched-chain amino acids and immunity J. Nutr. 136 2006 288S 293S
5. J.H. Park, and S.Y. Lee Fermentative production of branched chain amino acids: a focus on metabolic engineering Appl. Microbiol. Biotechnol. 85 2010 491 506
6. M. Vogt, S. Haas, S. Klaffl, T. Polen, L. Eggeling, O.J. van, and M. Bott Pushing product formation to its limit: metabolic engineering of Corynebacterium glutamicum for l-leucine overproduction Metab. Eng. 22 2014 40 52
7. J. Becker, O. Zelder, S. Häfner, H. Schröder, and C. Wittmann From zero to hero-design-based systems metabolic engineering of Corynebacterium glutamicum for l-lysine production Metab. Eng. 13 2011 159 168
8. J. Nakamura, S. Hirano, H. Ito, and M. Wachi Mutations of the Corynebacterium glutamicum NCgl1221 gene, encoding a mechanosensitive channel homolog, induce l-glutamic acid production Appl. Environ. Microbiol. 73 2007 4491 4498
9. M. Wada, N. Hijikata, R. Aoki, N. Takesue, and A. Yokota Enhanced valine production in Corynebacterium glutamicum with defective H+-ATPase and C-terminal truncated acetohydroxyacid synthase Biosci. Biotechnol. Biochem. 72 2008 2959 2965
10. S. Epelbaum, R.A. LaRossa, T.K. VanDyk, T. Elkayam, D.M. Chipman, and B. Ze'ev Branched-chain amino acid biosynthesis in Salmonella typhimurium: a quantitative analysis J. Bacteriol. 180 1998 4056 4067
11. L. Yin, X. Hu, D. Xu, J. Ning, J. Chen, and X. Wang Co-expression of feedback-resistant threonine dehydratase and acetohydroxy acid synthase increase l-isoleucine production in Corynebacterium glutamicum Metab. Eng. 14 2012 542 550
12. B. Blombach, M.E. Schreiner, T. Bartek, M. Oldiges, and B.J. Eikmanns Corynebacterium glutamicum tailored for high-yield l-valine production Appl. Microbiol. Biotechnol. 79 2008 471 479
13. rC genes J. Ind. Microbiol. Biotechnol. 39 2012 63 72
14. E. Radmacher, A. Vaitsikova, U. Burger, K. Krumbach, H. Sahm, and L. Eggeling Linking central metabolism with increased pathway flux: l-valine accumulation by Corynebacterium glutamicum Appl. Environ. Microbiol. 68 2002 2246 2250
15. B. Blombach, S. Hans, B. Bathe, and B.J. Eikmanns Acetohydroxyacid synthase, a novel target for improvement of l-lysine production by Corynebacterium glutamicum Appl. Environ. Microbiol. 75 2009 419 427
16. D. Park, K.E. Lee, C.H. Baek, I.H. Kim, J.H. Kwon, W.K. Lee, K.H. Lee, B.S. Kim, S.H. Choi, and K.S. Kim Cyclo(Phe-Pro) modulates the expression of ompU in Vibrio spp J. Bacteriol. 188 2006 2214 2221
17. J. Sambrook, E.F. Fritsch, and T. Maniatis Molecular Cloning: A Laboratory Manual 2nd ed. 1989 Cold Spring Harbor Laboratory Press New York
18. Y. Tan, D. Xu, Y. Li, and X. Wang Construction of a novel sacB-based system for marker-free gene deletion in Corynebacterium glutamicum Plasmid 67 2012 44 52
19. S. Simionatto, S.B. Marchioro, V. Galli, T.D. Luerce, D.D. Hartwig, A.N. Moreira, and O.A. Dellagostin Efficient site-direct mutagenesis using an overlap extension-PCR method for expressing Mycoplasma hyopneumoniae genes in Escherichia coli Microbiol. Meth. 79 2009 101 105
20. M.M. Bradford A dye binding assay for protein Anal. Biochem. 72 1976 248 254
21. C. Zhang, Z. Kang, J. Zhang, G. Du, J. Chen, and X. Yu Construction and application of novel feedback-resistant 3-deoxy-d-arabino-heptulosonate-7-phosphate synthases by engineering the N-terminal domain for l-phenylalanine synthesis FEMS Microbiol. Lett. 353 2014 11 18
22. C. Zhang, J. Zhang, Z. Kang, G. Du, X. Yu, T. Wang, and J. Chen Enhanced production of l-phenylalanine in Corynebacterium glutamicum due to the introduction of Escherichia coli wild-type gene aroH J. Ind. Microbiol. Biotechnol. 40 2013 643 651
23. M. Suchanek, A. Radzikowska, and C. Thiele Photo-leucine and photo-methionine allow identification of protein-protein interactions in living cells Nat. Methods 2 2005 261 268
24. V. Elisáková, M. Pátek, J. Holátko, J. Nesvera, D. Leyval, J.L. Goergen, and S. Delaunay Feedback-resistant acetohydroxy acid synthase increases valine production in Corynebacterium glutamicum Appl. Environ. Microbiol. 71 2005 207 213
25. A. Kaplun, M. Vyazmensky, Y. Zherdev, I. Belenky, A. Slutzker, S. Mendel, Z. Barak, D.M. Chipman, and B. Shaanan Structure of the regulatory subunit of acetohydroxyacid synthase isozyme III from Escherichia coli J. Mol. Biol. 357 2006 951 963
26. A. Slutzker, M. Vyazmensky, D.M. Chipman, and Z. Barak Role of the C-terminal domain of the regulatory subunit of AHAS isozyme III: use of random mutagenesis with in vivo reconstitution (REM-ivrs) Biochim. Biophys. Acta 2011 1814 449 455