Construction of a novel sacB-based system for marker-free gene deletion in Corynebacterium glutamicum

Plasmid

Volumn 67, Issue 1, 2012, Pages 44-52

  Tan, Yanzhen ^a   Xu, Daqing ^b   Li, Ye ^a   Wang, Xiaoyuan ^a  

^a JIANGNAN UNIVERSITY   (China)

^b AGRICULTURAL UNIVERSITY OF HEBEI   (China)

Author keywords

Corynebacterium glutamicum; Gene deletion; Marker free vector; PDXW 3; SacB

Indexed keywords

KANAMYCIN; SUCROSE;

ACEE GENE; ALLELE; ANTIBIOTIC RESISTANCE; ARTICLE; BACILLUS SUBTILIS; BACTERIAL CHROMOSOME; BACTERIAL GENE; BAMHI GENE; CONTROLLED STUDY; CORYNEBACTERIUM GLUTAMICUM; DNA FLANKING REGION; EAGI GENE; GENE CONSTRUCT; GENE DELETION; GENE DISRUPTION; GENE EXPRESSION; GENE VECTOR; GENETIC RECOMBINATION; MARKER GENE; MOLECULAR CLONING; NHEI GENE; NONHUMAN; NOTI GENE; ORIE GENE; PLACM GENE; PROMOTER REGION; REPLICON; RESTRICTION SITE; SACB GENE; SACI GENE; SALI GENE; XHOI GENE;

BASE SEQUENCE; CHROMOSOMES, BACTERIAL; CLONING, MOLECULAR; CORYNEBACTERIUM GLUTAMICUM; DNA, BACTERIAL; ESCHERICHIA COLI; GENE DELETION; GENES, BACTERIAL; GENETIC VECTORS; HOMOLOGOUS RECOMBINATION; MOLECULAR SEQUENCE DATA; PLASMIDS; POLYMERASE CHAIN REACTION; PROMOTER REGIONS, GENETIC; REPLICATION ORIGIN; REPLICON;

BACILLUS SUBTILIS; CORYNEBACTERIUM GLUTAMICUM; ESCHERICHIA COLI;

EID: 83555163727     PISSN: 0147619X     EISSN: 10959890     Source Type: Journal    
DOI: 10.1016/j.plasmid.2011.11.001     Document Type: Article

References (33)

1. Ananthalakshmy V.K., Gunasekaran P. Overproduction of levan in Zymomonas mobilis by using cloned sacB gene. Enzyme Microb. Technol. 1999, 25:109-115.
2. Beck E., Ludwig G., Auerswald E.A., Reiss B., Schaller H. Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5. Gene 1982, 19:327-336.
3. Blombach B., Schreiner M.E., Holatko J., Bartek T., Oldiges M., Eikmanns B.J. l-Valine production with pyruvate dehydrogenase complex-deficient Corynebacterium glutamicum. Appl. Environ. Microbiol. 2007, 73:2079-2084.
4. Burkovski A. Corynebacteria: Genomics and Molecular Biology 2008, Caister Academic Press, Norfolk.
5. Cha-aim K., Fukunaga T., Hoshida H., Akada R. Reliable fusion PCR mediated by GC-rich overlap sequences. Gene 2009, 434:43-49.
6. Eggeling L., Bott M. Handbook of Corynebacterium glutamicum 2005, Taylor & Francis, Boca Raton.
7. Gay P., Le Coq D., Steinmetz M., Berkelman T., Kado C.I. Positive selection procedure for entrapment of insertion sequence elements in Gram-negative bacteria. J. Bacteriol. 1985, 164:918-921.
8. Gerstmeir R., Wendisch V.F., Schnicke S., Ruan H., Farwick M., Reinscheid D., Eikmanns B.J. Acetate metabolism and its regulation in Corynebacterium glutamicum. J. Biotechnol. 2003, 104:99-122.
9. Holatko J., Elisakova V., Prouza M., Sobotka M., Nesvera J., Patek M. Metabolic engineering of the l-valine biosynthesis pathway in Corynebacterium glutamicum using promoter activity modulation. J. Biotechnol. 2009, 139:203-210.
10. Inui M., Tsuge Y., Suzuki N., Vertes A.A., Yukawa H. Isolation and characterization of a native composite transposon, Tn14751, carrying 17.4 kilobases of Corynebacterium glutamicum chromosomal DNA. Appl. Environ. Microbiol. 2005, 71:407-416.
11. Jager W., Schafer A., Puhler A., Labes G., Wohlleben W. Expression of the Bacillus subtilis sacB gene leads to sucrose sensitivity in the Gram-positive bacterium Corynebacterium glutamicum but not in Streptomyces lividans. J. Bacteriol. 1992, 174:5462-5465.
12. Keilhauer C., Eggeling L., Sahm H. Isoleucine synthesis in Corynebacterium glutamicum: molecular analysis of the ilvB-ilvN-ilvC operon. J. Bacteriol. 1993, 175:5595-5603.
13. Kinoshita S., Udaka S., Shimono M. Studies on the amino acid fermentation. Part 1. Production of l-glutamic acid by various microorganisms. J. Gen. Appl. Microbiol. 2004, 50:331-343.
14. Menkel E., Thierbach G., Eggeling L., Sahm H. Influence of increased aspartate availability on lysine formation by a recombinant strain of Corynebacterium glutamicum and utilization of fumarate. Appl. Environ. Microbiol. 1989, 55:684-688.
15. Mishra A.K., Alderwick L.J., Rittmann D., Wang C., Bhatt A., Jacobs W.R., Takayama K., Eggeling L., Besra G.S. Identification of a novel alpha(1→6) mannopyranosyltransferase MptB from Corynebacterium glutamicum by deletion of a conserved gene, NCgl1505, affords a lipomannan- and lipoarabinomannan-deficient mutant. Mol. Microbiol. 2008, 68:1595-1613.
16. Okibe N., Suzuki N., Inui M., Yukawa H. Efficient markerless gene replacement in Corynebacterium glutamicum using a new temperature-sensitive plasmid. J. Microbiol. Meth. 2011, 85:155-163.
17. Patek M., Eikmanns B.J., Patek J., Sahm H. Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif. Microbiology 1996, 142(Pt 5):1297-1309.
18. Pelicic V., Reyrat J.M., Gicquel B. Expression of the Bacillus subtilis sacB gene confers sucrose sensitivity on mycobacteria. J Bacteriol. 1996, 178:1197-1199.
19. Sambrook J., Russell D.W. Molecular Cloning: A Laboratory Manual 2001, Cold Spring Harbor Laboratory Press, New York.
20. Sanchez H., Cozar M.C., Martinez-Jimenez M.I. Targeting the Bacillus subtilis genome: an efficient and clean method for gene disruption. J. Microbiol. Meth. 2007, 70:389-394.
21. Schafer A., Tauch A., Jager W., Kalinowski J., Thierbach G., Puhler A. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene 1994, 145:69-73.
22. Schreiner M.E., Fiur D., Holatko J., Patek M., Eikmanns B.J. E1 enzyme of the pyruvate dehydrogenase complex in Corynebacterium glutamicum: molecular analysis of the gene and phylogenetic aspects. J. Bacteriol. 2005, 187:6005-6018.
23. Schreiner M.E., Riedel C., Holatko J., Patek M., Eikmanns B.J. Pyruvate:quinone oxidoreductase in Corynebacterium glutamicum: molecular analysis of the pqo gene, significance of the enzyme, and phylogenetic aspects. J. Bacteriol. 2006, 188:1341-1350.
24. Schwarzer A., Puhler A. Manipulation of Corynebacterium glutamicum by gene disruption and replacement. Biotechnology (NY) 1991, 9:84-87.
25. Srivastava P., Deb J.K. Gene expression systems in corynebacteria. Protein Expr. Purif. 2005, 40:221-229.
26. Steinmetz M., Le Coq D., Aymerich S., Gonzy-Treboul G., Gay P. The DNA sequence of the gene for the secreted Bacillus subtilis enzyme levansucrase and its genetic control sites. Mol. Gen. Genet. 1985, 200:220-228.
27. van der Geize R., de Jong W., Hessels G.I., Grommen A.W., Jacobs A.A., Dijkhuizen L. A novel method to generate unmarked gene deletions in the intracellular pathogen Rhodococcus equi using 5-fluorocytosine conditional lethality. Nucleic Acids Res. 2008, 36:e151.
28. van der Geize R., Hessels G.I., Dijkhuizen L. Molecular and functional characterization of the kstD2 gene of Rhodococcus erythropolis SQ1 encoding a second 3-ketosteroid Delta(1)-dehydrogenase isoenzyme. Microbiology 2002, 148:3285-3292.
29. van der Rest M.E., Lange C., Molenaar D. A heat shock following electroporation induces highly efficient transformation of Corynebacterium glutamicum with xenogeneic plasmid DNA. Appl. Microbiol. Biotechnol. 1999, 52:541-545.
30. Vertes A.A., Hatakeyama K., Inui M., Kobayashi M., Kurusu Y., Yukawa H. Replacement recombination in coryneform bacteria: high efficiency integration requirement for non-methylated plasmid DNA. Biosci. Biotechnol. Biochem. 1993, 57:2036-2038.
31. Xu D., Tan Y., Huan X., Hu X., Wang X. Construction of a novel shuttle vector for use in Brevibacterium flavum, an industrial amino acid producer. J. Microbiol. Meth. 2010, 80:86-92.
32. Xu D., Tan Y., Li Y., Wang X. Construction of a novel promoter-probe vector and its application for screening strong promoter for Brevibacterium flavum metabolic engineering. World J. Microbiol. Biotechnol. 2011, 27:961-968.
33. Xu D., Tan Y., Shi F., Wang X. An improved shuttle vector constructed for metabolic engineering research in Corynebacterium glutamicum. Plasmid 2010, 64:85-91.