Immobilization of lipases on hydrophobic supports involves the open form of the enzyme

Enzyme and Microbial Technology

Volumn 71, Issue , 2015, Pages 53-57

  Manoel, Evelin A ^a,b   dos Santos, José C S ^b,c   Freire, Denise M G ^d   Rueda, Nazzoly ^e   Fernandez Lafuente, Roberto ^b  

^a FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

^b CSIC   (Spain)

^c FEDERAL UNIVERSITY OF CEARÁ   (Brazil)

^d Programa de Pós Graduação em Bioquímica Departamento de Bioquímica   (Brazil)

^e UNIVERSIDAD INDUSTRIAL DE SANTANDER   (Colombia)

Author keywords

Detergents; Interfacial activation; Ionic strength; Irreversible inhibition; Lipase immobilization; Octyl agarose

Indexed keywords

DETERGENTS; ENZYME IMMOBILIZATION; LIPASES; SOAPS (DETERGENTS); SODIUM COMPOUNDS;

AGAROSE; HYDROPHOBIC SUPPORTS; INTERFACIAL ACTIVATION; IRREVERSIBLE INHIBITIONS; LIPASE IMMOBILIZATION; LIPASE PREPARATIONS; SODIUM PHOSPHATE BUFFER; THERMOMYCES LANUGINOSUS;

IONIC STRENGTH;

4 NITROPHENYL PHOSPHATE; AGAROSE; CYANOGEN BROMIDE; DETERGENT; IMMOBILIZED ENZYME; SODIUM DIHYDROGEN PHOSPHATE; TRIACYLGLYCEROL LIPASE; BACTERIAL PROTEIN; FUNGAL PROTEIN; OCTYL-SEPHAROSE CL-4B; SEPHAROSE;

ARTICLE; BURKHOLDERIA CEPACIA; COVALENT BOND; ENZYME CONFORMATION; ENZYME IMMOBILIZATION; ENZYME INHIBITION; HYDROPHOBICITY; IONIC STRENGTH; NONHUMAN; THERMOMYCES LANUGINOSUS; ANALOGS AND DERIVATIVES; ANTAGONISTS AND INHIBITORS; ASCOMYCETES; CHEMICAL PHENOMENA; CHEMISTRY; ENZYMOLOGY; KINETICS; METABOLISM; OSMOLARITY; PROTEIN CONFORMATION;

BURKHOLDERIA CEPACIA; THERMOMYCES LANUGINOSUS;

ASCOMYCOTA; BACTERIAL PROTEINS; BURKHOLDERIA CEPACIA; CYANOGEN BROMIDE; ENZYMES, IMMOBILIZED; FUNGAL PROTEINS; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; KINETICS; LIPASE; OSMOLAR CONCENTRATION; PROTEIN CONFORMATION; SEPHAROSE;

EID: 84923359729     PISSN: 01410229     EISSN: 18790909     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2015.02.001     Document Type: Article

References (53)

1. Jaeger K.E., Eggert T. Lipases for biotechnology. Curr Opin Biotechnol 2002, 13:390-397.
2. Sharma R., Chisti Y., Banerjee U.C. Production, purification, characterization, and applications of lipases. Biotechnol Adv 2001, 19:627-662.
3. Jaeger K.E., Reetz M.T. Microbial lipases form versatile tools for biotechnology. Trends Biotechnol 1998, 16:396-403.
4. Schmid R.D., Verger R. Lipases interfacial enzymes with attractive applications. Angew Chem Int Ed 1998, 37:1609-1633.
5. Pandey A., Benjamin S., Soccol C.R., Nigam P., Krieger N., Soccol V.T. The realm of microbial lipases in biotechnology. Biotechnol Appl Biochem 1999, 29(Pt 2):119-131.
6. Zhang J., Shi H., Wu D., Xing Z., Zhang A., Yang Y., et al. Recent developments in lipase-catalyzed synthesis of polymeric materials. Process Biochem 2014, 49:797-806.
7. Adlercreutz P. Immobilisation and application of lipases in organic media. Chem Soc Rev 2013, 42:6406-6436.
8. Chibata I., Tosa T., Sato T. Biocatalysis immobilized cells and enzymes. J Mol Catal 1986, 37:1-24.
9. Hartmeier W. Immobilized biocatalysts? From simple to complex systems. Trends Biotechnol 1985, 3:149-153.
10. Katchalski-Katzir E. Immobilized enzymes-learning from past successes and failures. Trends Biotechnol 1993, 11:471-478.
11. Kennedy J.F., Melo E.H.M., Jumel K. Immobilized enzymes and cells. Chem Eng Prog 1990, 86:81-89.
12. Klibanov A.M. Immobilized enzymes and cells as practical catalysts. Science 1983, 219:722-727.
13. Brady D., Jordaan J. Advances in enzyme immobilisation. Biotechnol Lett 2009, 31:1639-1650.
14. Fernandez-Lafuente R. Stabilization of multimeric enzymes: strategies to prevent subunit dissociation. Enzyme Microb Technol 2009, 45:405-418.
15. Mateo C., Palomo J.M., Fernandez-Lorente G., Guisan J.M., Fernandez-Lafuente R. Improvement of enzyme activity, stability and selectivity via immobilization techniques. Enzyme Microb Technol 2007, 40:1451-1463.
16. Petkar M., Lali A., Caimi P., Daminati M. Immobilization of lipases for non-aqueous synthesis. J Mol Catal B: Enzym 2006, 39:83-90.
17. Rodrigues R.C., Ortiz C., Berenguer-Murcia Á., Torres R., Fernández-Lafuente R. Modifying enzyme activity and selectivity by immobilization. Chem Soc Rev 2013, 42:6290-6307.
18. Hernandez K., Fernandez-Lafuente R. Control of protein immobilization: coupling immobilization and site-directed mutagenesis to improve biocatalyst or biosensor performance. Enzyme Microb Technol 2011, 48:107-122.
19. Guzik U., Hupert-Kocurek K., Wojcieszyńska D. Immobilization as a strategy for improving enzyme properties-application to oxidoreductases. Molecules 2014, 19:8995-9018.
20. Garcia-Galan C., Berenguer-Murcia Á., Fernandez-Lafuente R., Rodrigues R.C. Potential of different enzyme immobilization strategies to improve enzyme performance. Adv Synth Catal 2011, 353:2885-2904.
21. Barbosa O., Torres R., Ortiz C., Berenguer-Murcia A., Rodrigues R.C., Fernandez-Lafuente R. Heterofunctional supports in enzyme immobilization: from traditional immobilization protocols to opportunities in tuning enzyme properties. Biomacromolecules 2013, 14:2433-2462.
22. Verger R. Interfacial activation of lipases: facts and artifacts. Trends Biotechnol 1997, 15:32-38.
23. Cambillau C., Longhi S., Nicolas A., Martinez C. Acyl glycerol hydrolases: inhibitors, interface and catalysis. Curr Opin Struct Biol 1996, 6:449-455.
24. Brzozowski A.M., Derewenda U., Derewenda Z.S., Dodson G.G., Lawson D.M., Turkenburg J.P., et al. A model for interfacial activation in lipases from the structure of a fungal lipase-inhibitor complex. Nature 1991, 351:491-494.
25. Kim K.K., Song H.K., Shin D.H., Hwang K.Y., Suh S.W. The crystal structure of a triacylglycerol lipase from Pseudomonas cepacia reveals a highly open conformation in the absence of a bound inhibitor. Structure 1997, 5:173-185.
26. Jaeger K.E., Ransac S., Koch H.B., Ferrato F., Dijkstra B.W. Topological characterization and modeling of the 3D structure of lipase from Pseudomonas aeruginosa. FEBS Lett 1993, 332:143-149.
27. Cygler M., Schrag J.D. Structure and conformational flexibility of Candida rugosa lipase. Biochim Biophys Acta 1999, 1441:205-214.
28. Bastida A., Sabuquillo P., Armisen P., Fernández-Lafuente R., Huguet J., Guisán J.M. A single step purification, immobilization, and hyperactivation of lipases via interfacial adsorption on strongly hydrophobic supports. Biotechnol Bioeng 1998, 58:486-493.
29. Blanco R.M., Terreros P., Fernández-Pérez M., Otero C., Díaz-González G. Functionalization of mesoporous silica for lipase immobilization. J Mol Catal B: Enzym 2004, 30:83-93.
30. Bosley J.A., Clayton J.C. Blueprint for a lipase support: use of hydrophobic controlled-pore glasses as model systems. Biotechnol Bioeng 1994, 43:934-938.
31. Fernandez-Lafuente R., Armisén P., Sabuquillo P., Fernández-Lorente G., Guisán M.J. Immobilization of lipases by selective adsorption on hydrophobic supports. Chem Phys Lipids 1998, 93:185-197.
32. Baron A.M., Barouh N., Barea B., Villeneuve P., Mitchell D.A., Krieger N. Transesterification of castor oil in a solvent-free medium using the lipase from Burkholderia cepacia LTEB11 immobilized on a hydrophobic support. Fuel 2014, 117:458-462.
33. Du C., Zhang G., Wang Z., Li L., Tang J., Wang L. Application of ring-opening metathesis polymerization in study of polymer molecular weight-mediated catalytic properties of immobilized lipase. Chin Sci Bull 2009, 54:382-386.
34. Du C., Zhao B., Li C., Wang P., Wang Z., Tang J., et al. Improvement of the enantioselectivity and activity of lipase from Pseudomonas sp. via adsorption on a hydrophobic support: kinetic resolution of 2-octanol 2009.
35. Palomo J.M., Fuentes M., Fernández-Lorente G., Mateo C., Guisan J.M., Fernández-Lafuente R. General trend of lipase to self-assemble giving bimolecular aggregates greatly modifies the enzyme functionality. Biomacromolecules 2003, 4:1-6.
36. Fernández-Lorente G., Palomo J.M., Fuentes M., Mateo C., Guisán J.M., Fernández-Lafuente R. Self-assembly of Pseudomonas fluorescens lipase into bimolecular aggregates dramatically affects functional properties. Biotechnol Bioeng 2003, 82:232-237.
37. Palomo J.M., Ortiz C., Fuentes M., Fernandez-Lorente G., Guisan J.M., Fernandez-Lafuente R. Use of immobilized lipases for lipase purification via specific lipase-lipase interactions. J Chromatogr A 2004, 1038:267-273.
38. Palomo J.M., Ortiz C., Fernández-Lorente G., Fuentes M., Guisán J.M., Fernández-Lafuente R. Lipase-lipase interactions as a new tool to immobilize and modulate the lipase properties. Enzyme Microb Technol 2005, 36:447-454.
39. Fernandez-Lorente G., Palomo J.M., Cabrera Z., Fernandez-Lafuente R., Guisán J.M. Improved catalytic properties of immobilized lipases by the presence of very low concentrations of detergents in the reaction medium. Biotechnol Bioeng 2007, 97:242-250.
40. Hermoso J., Pignol D., Kerfelec B., Crenon I., Chapus C., Fontecilla-Camps J.C. Lipase activation by nonionic detergents. The crystal structure of the porcine lipase-colipase-tetraethylene glycol monooctyl ether complex. J Biol Chem 1996, 271:18007-18016.
41. Fernández-Lorente G., Palomo J.M., Mateo C., Munilla R., Ortiz C., Cabrera Z., et al. Glutaraldehyde cross-linking of lipases adsorbed on aminated supports in the presence of detergents leads to improved performance. Biomacromolecules 2006, 7:2610-2615.
42. Mogensen J.E., Sehgal P., Otzen D.E. Activation, inhibition, and destabilization of Thermomyces lanuginosus lipase by detergents. Biochemistry 2005, 44:1719-1730.
43. Helistö P., Korpela T. Effects of detergents on activity of microbial lipases as measured by the nitrophenyl alkanoate esters method. Enzyme Microb Technol 1998, 23:113-117.
44. Jutila A., Zhu K., Patkar S.A., Vind J., Svendsen A., Kinnunen P.K. Detergent-induced conformational changes of Humicola lanuginosa lipase studied by fluorescence spectroscopy. Biophys J 2000, 78:1634-1642.
45. Carrasco-López C., Godoy C., de las Rivas B., Fernández-Lorente G., Palomo J.M., Guisán J.M., et al. Activation of bacterial thermo alkalophilic lipases is spurred by dramatic structural rearrangements. J Biol Chem 2009, 284:4365-4372.
46. Fernandez-Lafuente R. Lipase from Thermomyces lanuginosus: uses and prospects as an industrial biocatalyst. J Mol Catal B: Enzym 2010, 62:197-212.
47. Bora L., Gohain D., Das R. Recent advances in production and biotechnological applications of thermostable and alkaline bacterial lipases. J Chem Technol Biotechnol 2013, 88:1959-1970.
48. Chebil L., Humeau C., Falcimaigne A., Engasser J.M., Ghoul M. Enzymatic acylation of flavonoids. Process Biochem 2006, 41:2237-2251.
49. Pàmies O., Bäckvall J-E. Chemoenzymatic dynamic kinetic resolution. Trends Biotechnol 2004, 22:130-135.
50. Mateo C., Abian O., Bernedo M., Cuenca E., Fuentes M., Fernandez-Lorente G., et al. Some special features of glyoxyl supports to immobilize proteins. Enzyme Microb Technol 2005, 37:456-462.
51. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 1976, 72:248-254.
52. dos Santos J.C.S., Garcia-Galan C., Rodrigues R.C., de Sant' Ana H.B., Gonçalves L.R.B., Fernandez-Lafuente R. Improving the catalytic properties of immobilized Lecitase via physical coating with ionic polymers. Enzyme Microb Technol 2014, 60:1-8.
53. Garcia-Galan C., Barbosa O., Hernandez K., dos Santos J.C.S., Rodrigues R.C., Fernandez-Lafuente R. Evaluation of styrene-divinylbenzene beads as a support to immobilize lipases. Molecules 2014, 19:7629-7645.