Nuclemeter: A reaction-diffusion based method for quantifying nucleic acids undergoing enzymatic amplification

Scientific Reports

Volumn 4, Issue , 2014, Pages

  Liu, Changchun ^a   Sadik, Mohamed M ^a   Mauk, Michael G ^a   Edelstein, Paul H ^b   Bushman, Frederic D ^b   Gross, Robert ^b,c   Bau, Haim H ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b UNIVERSITY OF PENNSYLVANIA   (United States)

^c UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RECOMBINASE; VIRUS DNA;

COMPUTER SYSTEM; DEVICES; DNA SEQUENCE; GENETICS; HUMAN IMMUNODEFICIENCY VIRUS; ISOLATION AND PURIFICATION; MICROFLUIDIC ANALYSIS; NUCLEIC ACID AMPLIFICATION; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; VIRUS LOAD;

COMPUTER SYSTEMS; DNA, VIRAL; HIV; MICROFLUIDIC ANALYTICAL TECHNIQUES; NUCLEIC ACID AMPLIFICATION TECHNIQUES; RECOMBINASES; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA; VIRAL LOAD;

EID: 84923283457     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep07335     Document Type: Article

References (26)

1. Higuchi, R., Fockler, C., Dollinger, G. & Watson, R. Kinetic PCR analysis: realtime monitoring of DNA amplification reactions. Nat. Biotechnol. 11, 1026-1030 (1993).
2. Heid, C. A., Stevens, J., Livak, K. J. &Williams, P. M. Real time quantitative PCR. Genome Res. 6, 986-994 (1996).
3. Toumazou, C. et al. Simultaneous dna amplification and detection using a phsensing semiconductor system. Nat Methods. 10, 641-646 (2013).
4. Rouet, F. et al. Transfer and evaluation of an automated, low-cost real-time reverse transcription-PCR test for diagnosis and monitoring of human immunodeficiency virus type 1 infection in a West African resource-limited setting. J. Clin. Microbiol. 43, 2709-2717 (2005).
5. Granich, R. M., Gilks, C. F., Dye, C., De Cock, K. M. &Williams, B. G. Universal voluntary HIV testing with immediate antiretroviral therapy as a strategy for elimination of HIV transmission: a mathematical model. Lancet 373, 48-57 (2009).
6. Christensen, D. R. et al. Detection of biological threat agents by real-time PCR: comparison of assay performance on the R.A.P.I.D., the LightCycler, and the Smart Cycler platforms. Clin Chem. 52, 141-145 (2006).
7. Bell, A. S.&Ranford-Cartwright, L. C. Real-time quantitative PCR in parasitology. Trends Parasitol 18, 337-342 (2002).
8. Jaroenram, W., Kiatpathomchai, W. & Flegel, T. W. Rapid and sensitive detection of white spot syndrome virus by loop-mediated isothermal amplification combined with a lateral flow dipstick. Mol Cell Probes. 23, 65-70 (2009).
9. Rohrman, B. A., Leautaud, V., Molyneux, E. & Richards-Kortum, R. R. A Lateral Flow Assay for Quantitative Detection of Amplified HIV-1 RNA. PLoS ONE 7, e45611 (2012).
10. Roskos, K. et al. Simple system for isothermal DNA amplification coupled to lateral flow detection. PLoS One. 8, e69355 (2013).
11. Yager, P. et al. Microfluidic diagnostic technologies for global public health. Nature 442, 412-418 (2006).
12. Hart, R. W. et al. Point-of-care oral-based diagnostics. Oral Dis. 17, 745-52 (2011).
13. Song, Y. et al. Point-of-care technologies for molecular diagnostics using a drop of blood. Trends Biotechnol. 32, 132-139 (2014).
14. Niemz, A., Ferguson, T. M. & Boyle, D. S. Point-of-care nucleic acid testing for infectious diseases. Trends Biotechnol. 29, 240-250 (2011).
15. Zhang, C. & Xing, D. Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends. Nucleic Acids Res. 35, 4223-4237 (2007).
16. Lee, J. G. et al. Microchip-based one step DNA extraction and real-time PCR in one chamber for rapid pathogen identification. Lab Chip 6, 886-895 (2006).
17. Dimov, I. K. et al. Integrated microfluidic tmRNA purification and real-time NASBA device for molecular diagnostics. Lab Chip 8, 2071-2078 (2008).
18. Liu, C., Mauk, M. G., Hart, R., Qiu, X. & Bau, H. H. A self-heating cartridge for molecular diagnostics. Lab Chip 11, 2686-2692 (2011).
19. Selck, D. A., Karymov, M. A., Sun, B. & Ismagilov, R. F. Increased robustness of single-molecule counting with microfluidics, digital isothermal amplification, and a mobile phone versus real-time kinetic measurements. Analytical Chemistry 85, 11129-11136 (2013).
20. Notomi, T. et al. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 28, E63 (2000).
21. Tomita, N., Mori, Y., Kanda, H. & Notomi, T. Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products. Nat. Protoc. 3, 877-882 (2008).
22. Fisher, R. A. The wave of advance of advantageous genes. Ann. Eugenics 7, 353-369 (1937).
23. Liu, C. et al. A low-cost microfluidic chip for rapid genotyping of malariatransmitting mosquitoes. PLoS ONE 7, e42222 (2012).
24. Mao, H., Holden, M. A., You, M. & Cremer, P. S. Reusable platforms for high-throughput on-chip temperature gradient assays. Anal Chem. 74, 5071-5075 (2002).
25. Liu, C. et al. An isothermal amplification reactor with an integrated isolation membrane for point-of-care detection of infectious diseases. Analyst 136, 2069-2076 (2011).
26. Curtis, K. A., Rudolph, D. L. & Owen, S. M. Rapid detection of HIV-1 by reverse-transcription, loop-mediated isothermal amplification (RT-LAMP). J. Virol. Meth. 151, 264-270 (2008).