Microchip-based one step DNA extraction and real-time PCR in one chamber for rapid pathogen identification

Lab on a Chip

Volumn 6, Issue 7, 2006, Pages 886-895

  Lee, Jeong Gun ^a   Cheong, Kwang Ho ^a   Huh, Nam ^a   Kim, Suhyeon ^a   Choi, Jeong Woo ^b   Ko, Christopher ^a  

^a Samsung Advanced Institute of Technology (SAIT)   (South Korea)

^b Sogang University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

REAGENT;

ARTICLE; BACTERIAL CELL; BACTERIUM IDENTIFICATION; CARBOXY TERMINAL SEQUENCE; DEVICE; DIODE LASER; DNA EXTRACTION; ESCHERICHIA COLI; GRAM POSITIVE BACTERIUM; HEPATITIS B VIRUS; IRRADIATION; MAGNETISM; MICROCHIP ANALYSIS; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; TECHNIQUE; VIRUS IDENTIFICATION;

BACTERIA (MICROORGANISMS); HEPATITIS B VIRUS; POSIBACTERIA;

EID: 33745509562     PISSN: 14730197     EISSN: 14730189     Source Type: Journal    
DOI: 10.1039/b515876a     Document Type: Article

References (22)

1. B. M. Chassy A. Giuffrida Method for the lysis of Gram-positive, Asporogenous bacteria with lysozyme Appl. Environ. Microbiol. 1980 39 153 158
2. R. Pal M. Yang R. Kin B. N. Johnson N. Srivastava S. Z. Razzacki K. J. Chomistek D. C. Heldsinger R. M. Haque V. M. Ugaz R. K. Thwar Z. Chen K. Alfano M. B. Yim M. Krishnan A. O. Fuller R. G. Larson D. T. Burke M. A. Burns An integrated microfluidic device for influenza and other genetic analyses Lab Chip 2005 5 1024 1032
3. P. Belgrader D. Hansford G. T. A. Kovacs K. Venkateswaran R. Mariella F. Milanovich S. Nasarabadi M. Okuzumi F. Pourahmadi M. A. Northrup A minisonicator to rapidly disrupt bacterial spores for DNA analysis Anal. Chem. 1999 71 4232 4236
4. M. T. Taylor P. Belgrader B. J. Furman F. Pourahmadi G. T. A. Kovacs M. A. Northrup Lysing bacterial spores by sonication through a flexible interface in a microfluidics system Anal. Chem. 2001 73 492 496
5. A. Abolmaaty M. G. El-Shemy M. F. Khallaf R. E. Levin Effect of lysing methods and their variables on the yield of Escherichia coli O157:H7 DNA and its PCR amplification J. Microbiol. Methods 1998 34 133 141
6. I. Z. Shirgaonkar R. R. Lothe A. B. Pandit Comments on the mechanism of microbial cell disruption in high-pressure and high-speed device Biotechnol. Prog. 1998 14 657 660
7. E. Keshavaraz-Moore M. Hoare P. Dunnill Disruption of Baker's yeast in a high-pressure homogenizer: New evidence on mechanism Enzyme Microb. Technol. 1990 12 764 770
8. F. Han Y. Wang C. E. Sims M. Bachman R. Chang G. P. Li N. L. Allbritton Fast electrical lysis of cells for capillary electrophoresis Anal. Chem. 2003 75 3688 3696
9. J. Gao X. F. Yin Z. L. Fang Integration of single cell injection, cell lysis, separation and detection of intracellular constituents on a microfluidic chip Lab Chip 2004 4 24 52
10. P. R. C. Gascoyne J. V. Vykoukal Dielectrophoretic concepts for automated diagnostic instruments Proc. IEEE 2004 92 22 42
11. R. Boom C. J. A. Sol M. M. M. Salimans C. L. Jansen P. M. E. Wertheim-van Dillen J. van der Noordaa Rapid and simple method for purification of nucleic acids J. Clin. Microbiol. 1990 28 495 503
12. M. G. Elgort M. G. Hermann M. Erail J. D. Durschi K. V. Voelkerding R. E. Smith Extraction and amplification of genomic DNA from human blood on nanoporous aluminum oxide membranes Clin. Chem. 2004 50 1817 1819
13. R. W. Chen H. Piiparinen M. Seppanen P. Koskela S. Sarna M. Lappalainen Real-time PCR for detection and quantification of hepatitis B virus DNA J. Med. Virol. 2001 65 250 256
14. G. M. Hale M. R. Querry Optical constants of water in the 200 nm to 200 μm wavelength region Appl. Opt. 1973 12 555 563
15. L. Kou D. Labrie P. Chylek Refractive indices of water and ice in the 0.65-2.5 mm spectral range Appl. Opt. 1993 32 3531 3540
16. T. A. Slieman W. L. Nicholson Artificial and solar UV radiation induces strand breaks and cyclobutane pyrimidine dimers in Bacillus subtilis spore DNA Appl. Environ. Microbiol. 2000 66 199 205
17. Y. K. Cho J. Kim Y. Lee Y.-A. Kim K. Namkoong H. Lim K. W. Oh S. Kim J. Han C. Park Y. E. Pak C.-S. Ki J. R. Choi H.-K. Myeong C. Ko Clinical evaluation of micro-scale chip-based PCR system for rapid detection of hepatitis B virus Biosens. Bioelectron. 2006 21 2161 2169
18. K. W. Oh C. Park K. Namkoong J. Kim K.-S. Ock S. Kim Y.-A. Kim Y.-K. Cho C. Ko World-to-chip microfluidics interface with built-in valves for multichamber chip-based PCR assays Lab Chip 2005 5 845 850
19. ® Micro PCR) for hepatitis B virus DNA detection, Micro Total Analysis System 2004, Malmo, 2004, pp. 150-152
20. O. Hofmann K. Murray A. S. Wilkinson T. Cox A. Manz Laser induced disruption of bacterial spores on a microchip Lab Chip 2005 5 374 377
21. J. W. Hong V. Studer G. Hang W. F. Anderson S. R. Quake A nanoliter-scale nucleic acid processor with parallel architecture Nat. Biotechnol. 2004 22 435 439
22. M. A. Burns B. N. Johnson S. N. Brahmasandra K. Handique J. R. Webster M. Krishnan T. S. Sammarco P. M. Man D. Jones D. Heldsinger C. H. Mastrangelo D. T. Burke An integrated nanoliter analysis device Science 1998 282 484 487