Whole-genome sequence of Chryseobacterium oranimense, a colistin-resistant bacterium isolated from a cystic fibrosis patient in France

Antimicrobial Agents and Chemotherapy

Volumn 59, Issue 3, 2015, Pages 1696-1706

  Sharma, Poonam ^a   Gupta, Sushim Kumar ^a   Diene, Seydina M ^a   Rolain, Jean Marc ^a  

^a AIX MARSEILLE UNIVERSITÉ   (France)

Author keywords

[No Author keywords available]

Indexed keywords

AMIKACIN; AMOXICILLIN; AMOXICILLIN PLUS CLAVULANIC ACID; AZTREONAM; CEFOTAXIME; CEFOXITIN; CEFTAZIDIME; CEFTRIAXONE; CIPROFLOXACIN; COLISTIN; COTRIMOXAZOLE; GENTAMICIN; IMIPENEM; O ANTIGEN; OFLOXACIN; POLYKETIDE SYNTHASE; TICARCILLIN; TOBRAMYCIN; ZEAXANTHIN; ANTIINFECTIVE AGENT; BACTERIAL DNA; BACTERIAL PROTEIN; BETA LACTAMASE;

ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL GENOME; BACTERIAL GROWTH; BACTERIUM ISOLATE; BACTERIUM ISOLATION; CASE REPORT; CHILD; CHRYSEOBACTERIUM; CHRYSEOBACTERIUM GLEUM; CHRYSEOBACTERIUM ORANIMENSE; CYSTIC FIBROSIS; DNA BASE COMPOSITION; FRANCE; GENE INSERTION SEQUENCE; GENE SEQUENCE; GENOME SIZE; HUMAN; MINIMUM INHIBITORY CONCENTRATION; MIXED INFECTION; NONHUMAN; NUCLEOTIDE SEQUENCE; OPERON; PHYLOGENETIC TREE; PRESCHOOL CHILD; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; STENOTROPHOMONAS MALTOPHILIA; BACTERIUM IDENTIFICATION; DNA SEQUENCE; DRUG EFFECTS; FLAVOBACTERIACEAE INFECTIONS; GENETICS; MICROBIOLOGY; MULTIDRUG RESISTANCE; MUTATION; PHYLOGENY; PROCEDURES;

ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; BACTERIAL TYPING TECHNIQUES; BASE COMPOSITION; BETA-LACTAMASES; CHRYSEOBACTERIUM; COLISTIN; CYSTIC FIBROSIS; DNA, BACTERIAL; DRUG RESISTANCE, MULTIPLE, BACTERIAL; FLAVOBACTERIACEAE INFECTIONS; FRANCE; HUMANS; IMIPENEM; MUTATION; PHYLOGENY; SEQUENCE ANALYSIS, DNA;

EID: 84923240908     PISSN: 00664804     EISSN: 10986596     Source Type: Journal    
DOI: 10.1128/AAC.02417-14     Document Type: Article

References (68)

1. Rommens JM, Iannuzzi MC, Kerem B, Drumm ML, Melmer G, Dean M, Rozmahel R, Cole JL, Kennedy D, Hidaka N. 1989. Identification of the cystic fibrosis gene: chromosome walking and jumping. Science 245: 1059-1065. http://dx.doi.org/10.1126/science.2772657.
2. Riordan JR, Rommens JM, Kerem B, Alon N, Rozmahel R, Grzelczak Z, Zielenski J, Lok S, Plavsic N, Chou JL. 1989. Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA. Science 245:1066-1073. http://dx.doi.org/10.1126/science.2475911.
3. Lyczak JB, Cannon CL, Pier GB. 2002. Lung infections associated with cystic fibrosis. Clin Microbiol Rev 15:194-222. http://dx.doi.org/10.1128/CMR.15.2.194-222.2002.
4. Miller MB, Gilligan PH. 2002. Laboratory aspects of management of chronic pulmonary infections in patients with cystic fibrosis. J Clin Microbiol 41:4009-4015. http://dx.doi.org/10.1128/JCM.41.9.4009-4015.2003.
5. LiPuma JJ. 2010. The changing microbial epidemiology in cystic fibrosis. Clin Microbiol Rev 23:299-323. http://dx.doi.org/10.1128/CMR.00068-09.
6. Denton M, Kerr K, Mooney L, Keer V, Rajgopal A, Brownlee K, Arundel P, Conway S. 2002. Transmission of colistin-resistant Pseudomonas aeruginosa between patients attending a pediatric cystic fibrosis center. Pediatr Pulmonol 34:257-261. http://dx.doi.org/10.1002/ppul.10166.
7. Johansen HK, Moskowitz SM, Ciofu O, Pressler T, Hoiby N. 2008. Spread of colistin resistant non-mucoid Pseudomonas aeruginosa among chronically infected Danish cystic fibrosis patients. J Cyst Fibros 7:391-397. http://dx.doi.org/10.1016/j.jcf.2008.02.003.
8. Bittar F, Leydier A, Bosdure E, Toro A, Reynaud-Gaubert M, Boniface S, Stremler N, Dubus JC, Sarles J, Raoult D, Rolain JM. 2008. Inquilinus limosus and cystic fibrosis. Emerg Infect Dis 14:993-995. http://dx.doi.org/10.3201/eid1406.071355.
9. Menuet M, Bittar F, Stremler N, Dubus JC, Sarles J, Raoult D, Rolain JM. 2008. First isolation of two colistin-resistant emerging pathogens, Brevundimonas diminuta and Ochrobactrum anthropi, in a woman with cystic fibrosis: a case report. J Med Case Rep 2:373. http://dx.doi.org/10.1186/1752-1947-2-373.
10. Burns JL, Emerson J, Stapp JR, Yim DL, Krzewinski J, Louden L, Ramsey BW, Clausen CR. 1998. Microbiology of sputum from patients at cystic fibrosis centers in the United States. Clin Infect Dis 27:158-163. http://dx.doi.org/10.1086/514631.
11. Hogardt M, Schmoldt S, Gotzfried M, Adler K, Heesemann J. 2004. Pitfalls of polymyxin antimicrobial susceptibility testing of Pseudomonas aeruginosa isolated from cystic fibrosis patients. J Antimicrob Chemother 54:1057-1061. http://dx.doi.org/10.1093/jac/dkh470.
12. Spencer RC. 1995. The emergence of epidemic, multiple-antibioticresistant Stenotrophomonas (Xanthomonas) maltophilia and Burkholderia (Pseudomonas) cepacia. J Hosp Infect 30(Suppl):453-464.
13. Smith J, Han R, Mailman T, MacDonald N. 2012. Chryseobacterium indologenes: distinguishing pathogen from contaminant in a neonate. J Ped Infect Dis 7:31-35. http://dx.doi.org/10.3233/JPI-2012-0337.
14. Reynaud I, Chanteperdrix V, Broux C, Pavese P, Croize J, Maurin M, Stahl JP, Jacquot C. 2007. A severe form of Chryseobacterium indologenes pneumonia in an immunocompetent patient. Med Mal Infect 37:762-764. http://dx.doi.org/10.1016/j.medmal.2007.01.006.
15. Chou DW, Wu SL, Lee CT, Tai FT, Yu WL. 2011. Clinical characteristics, antimicrobial susceptibilities, and outcomes of patients with Chryseobacterium indologenes bacteremia in an intensive care unit. Jpn J Infect Dis 64:520-524.
16. Chen FL, Wang GC, Teng SO, Ou TY, Yu FL, Lee WS. 2013. Clinical and epidemiological features of Chryseobacterium indologenes infections: analysis of 215 cases. J Microbiol Immunol Infect 46(6):425-432. http://dx.doi.org/10.1016/j.jmii.2012.08.007.
17. Vandamme P, Bernardet JF, Segers P, Kersters K, Holmes B. 1994. New perspectives in the classification of the flavobacteria: description of Chryseobacterium gen. nov., Bergeyella gen. nov, and Empedobacter norn. rev. Int J Syst Bacteriol 44:827-831.
18. Maravic A, Skocibusic M, Samanic I, Puizina J. 2013. Profile and multidrug resistance determinants of Chryseobacterium indologenes from seawater and marine fauna. World J Microbiol Biotechnol 29:515-522. http://dx.doi.org/10.1007/s11274-012-1205-0.
19. Lambiase A, Raia V, Del Pezzo M, Sepe A, Carnovale V, Rossano F. 2006. Microbiology of airway disease in a cohort of patients with cystic fibrosis. BMC Infect Dis 6:4. http://dx.doi.org/10.1186/1471-2334-6-4.
20. Lambiase A, Del Pezzo M, Raia V, Sepe A, Ferri P, Rossano F. 2007. Chryseobacterium respiratory tract infections in patients with cystic fibrosis. J Infect 55:518-523. http://dx.doi.org/10.1016/j.jinf.2007.08.002.
21. Lambiase A, Raia V, Stefani S, Sepe A, Ferri P, Buonpensiero P, Rossano F, Del Pezzo M. 2007. Burkholderia cepacia complex infection in a cohort of Italian patients with cystic fibrosis. J Microbiol 45:275-279.
22. Zankari E, Hasman H, Kaas RS, Seyfarth AM, Agerso Y, Lund O, Larsen MV, Aarestrup FM. 2013. Genotyping using whole-genome sequencing is a realistic alternative to surveillance based on phenotypic antimicrobial susceptibility testing. J Antimicrob Chemother 68:771-777. http://dx.doi.org/10.1093/jac/dks496.
23. Snitkin ES, Zelazny A, Gupta J, Palmore TN, Murray PR, Segre JA. 2013. Genomic insights into the fate of colistin resistance and Acinetobacter baumannii during patient treatment. Genome Res 23(7):1155-1162. http://dx.doi.org/10.1101/gr.154328.112.
24. Seng P, Drancourt M, Gouriet F, La Scola B, Fournier PE, Rolain JM, Raoult D. 2009. Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-offlight mass spectrometry. Clin Infect Dis 49:543-551. http://dx.doi.org/10.1086/600885.
25. Lee K, Chong Y, Shin HB, Kim YA, Yong D, Yum JH. 2001. Modified Hodge and EDTA-disk synergy tests to screen metallo-beta-lactamaseproducing strains of Pseudomonas and Acinetobacter species. Clin Microbiol Infect 7:88-91. http://dx.doi.org/10.1046/j.1469-0691.2001.00204.x.
26. Yong D, Lee K, Yum JH, Shin HB, Rossolini GM, Chong Y. 2002. Imipenem-EDTA disk method for differentiation of metallo-betalactamase-producing clinical isolates of Pseudomonas spp. and Acinetobacter spp. J Clin Microbiol 40:3798-3801. http://dx.doi.org/10.1128/JCM.40.10.3798-3801.2002.
27. Bernard L, Vaudaux P, Rohner P, Huggler E, Armanet M, Pittet D, Lew DP, Schrenzel J. 2004. Comparative analysis and validation of different assays for glycopeptide susceptibility among methicillin-resistant Staphylococcus aureus strains. J Microbiol Methods 57:231-239. http://dx.doi.org/10.1016/j.mimet.2004.01.012.
28. Aziz RK, Bartels D, Best AA, DeJongh M, Disz T, Edwards RA, Formsma K, Gerdes S, Glass EM, Kubal M, Meyer F, Olsen GJ, Olson R, Osterman AL, Overbeek RA, McNeil LK, Paarmann D, Paczian T, Parrello B, Pusch GD, Reich C, Stevens R, Vassieva O, Vonstein V, Wilke A, Zagnitko O. 2008. The RAST Server: rapid annotations using subsystems technology. BMC Genomics 9:75. http://dx.doi.org/10.1186/1471-2164-9-75.
29. Gupta SK, Padmanabhan B, Diene SM, Lopez-Rojas R, Kempf M, Landraud L, Rolain J-M. 2014. ARG-ANNOT (Antibiotic Resistance Gene-ANNOTation), a new bioinformatic tool to discover antibiotic resistance genes in bacterial genomes. Antimicrob Agents Chemother 58: 212-220. http://dx.doi.org/10.1128/AAC.01310-13.
30. Hantsis-Zacharov E, Shaked T, Senderovich Y, Halpern M. 2008. Chryseobacterium oranimense sp. nov., a psychrotolerant, proteolytic and lipolytic bacterium isolated from raw cow's milk. Int J Syst Evol Microbiol 58:2635-2639. http://dx.doi.org/10.1099/ijs.0.65819-0.
31. Alikhan NF, Petty NK, Ben Zakour NL, Beatson SA. 2011. BLAST Ring Image Generator (BRIG): simple prokaryote genome comparisons. BMC Genomics 12:402. http://dx.doi.org/10.1186/1471-2164-12-402.
32. Woodford N, Palepou MF, Babini GS, Holmes B, Livermore DM. 2000. Carbapenemases of Chryseobacterium (Flavobacterium) meningosepticum: distribution of blaB and characterization of a novel metallobeta-lactamase gene, blaB3, in the type strain, NCTC 10016. Antimicrob Agents Chemother 44:1448-1452. http://dx.doi.org/10.1128/AAC.44.6.1448-1452.2000.
33. Yasmin S, Garcia G, Sylvester T, Sunenshine R. 2013. Chryseobacterium indologenes in a woman with metastatic breast cancer in the United States of America: a case report. J Med Case Rep 7:190. http://dx.doi.org/10.1186/1752-1947-7-190.
34. Coenye T, Goris J, Spilker T, Vandamme P, LiPuma JJ. 2002. Characterization of unusual bacteria isolated from respiratory secretions of cystic fibrosis patients and description of Inquilinus limosus gen. nov., sp. nov. J Clin Microbiol 40:2062-2069. http://dx.doi.org/10.1128/JCM.40.6.2062-2069.2002.
35. Brown SD, Utturkar SM, Klingeman DM, Johnson CM, Martin SL, Land ML, Lu TY, Schadt CW, Doktycz MJ, Pelletier DA. 2012. Twentyone genome sequences from Pseudomonas species and 19 genome sequences from diverse bacteria isolated from the rhizosphere and endosphere of Populus deltoides. J Bacteriol 194:5991-5993. http://dx.doi.org/10.1128/JB.01243-12.
36. Bhosale P, Bernstein PS. 2004. Beta-carotene production by Flavobacterium multivorum in the presence of inorganic salts and urea. J Ind Microbiol Biotechnol 31:565-571. http://dx.doi.org/10.1007/s10295-004-0187-9.
37. Krishnadev N, Meleth AD, Chew EY. 2010. Nutritional supplements for age-related macular degeneration. Curr Opin Ophthalmol 21:184-189. http://dx.doi.org/10.1097/ICU.0b013e32833866ee.
38. Raetz CR, Reynolds CM, Trent MS, Bishop RE. 2007. Lipid A modification systems in Gram-negative bacteria. Annu Rev Biochem 76:295-329. http://dx.doi.org/10.1146/annurev.biochem.76.010307.145803.
39. Bornet C, Davin-Regli A, Bosi C, Pages JM, Bollet C. 2000. Imipenem resistance of Enterobacter aerogenes mediated by outer membrane permeability. J Clin Microbiol 38:1048-1052.
40. Thiolas A, Bollet C, La Scola B, Raoult D, Pages JM. 2005. Successive emergence of Enterobacter aerogenes strains resistant to imipenem and colistin in a patient. Antimicrob Agents Chemother 49:1354-1358. http://dx.doi.org/10.1128/AAC.49.4.1354-1358.2005.
41. Ingram BO, Sohlenkamp C, Geiger O, Raetz CR. 2010. Altered lipid A structures and polymyxin hypersensitivity of Rhizobium etli mutants lacking the LpxE and LpxF phosphatases. Biochim Biophys Acta 1801:593-604. http://dx.doi.org/10.1016/j.bbalip.2010.02.001.
42. Paulsen IT, Beness AM, Saier MH, Jr. 1997. Computer-based analyses of the protein constituents of transport systems catalysing export of complex carbohydrates in bacteria. Microbiology 143(Part 8):2685-2699.
43. Arakawa Y, Wacharotayankun R, Nagatsuka T, Ito H, Kato N, Ohta M. 1995. Genomic organization of the Klebsiella pneumoniae cps region responsible for serotype K2 capsular polysaccharide synthesis in the virulent strain Chedid. J Bacteriol 177:1788-1796.
44. Perna NT, Plunkett G, III, Burland V, Mau B, Glasner JD, Rose DJ, Mayhew GF, Evans PS, Gregor J, Kirkpatrick HA, Posfai G, Hackett J, Klink S, Boutin A, Shao Y, Miller L, Grotbeck EJ, Davis NW, Lim A, Dimalanta ET, Potamousis KD, Apodaca J, Anantharaman TS, Lin J, Yen G, Schwartz DC, Welch RA, Blattner FR. 2001. Genome sequence of enterohaemorrhagic Escherichia coli O157:H7. Nature 409:529-533. http://dx.doi.org/10.1038/35054089.
45. Reeves PR, Hobbs M, Valvano MA, Skurnik M, Whitfield C, Coplin D, Kido N, Klena J, Maskell D, Raetz CR, Rick PD. 1996. Bacterial polysaccharide synthesis and gene nomenclature. Trends Microbiol 4:495-503. http://dx.doi.org/10.1016/S0966-842X(97)82912-5.
46. Lacour S, Bechet E, Cozzone AJ, Mijakovic I, Grangeasse C. 2008. Tyrosine phosphorylation of the UDP-glucose dehydrogenase of Escherichia coli is at the crossroads of colanic acid synthesis and polymyxin resistance. PLoS One 3:e3053. http://dx.doi.org/10.1371/journal.pone.0003053.
47. Trent MS, Ribeiro AA, Lin S, Cotter RJ, Raetz CR. 2001. An inner membrane enzyme in Salmonella and Escherichia coli that transfers 4-amino-4-deoxy-L-arabinose to lipid A: induction on polymyxin-resistant mutants and role of a novel lipid-linked donor. J Biol Chem 276:43122-43131. http://dx.doi.org/10.1074/jbc.M106961200.
48. MacLean LL, Perry MB, Crump EM, Kay WW. 2003. Structural characterization of the lipopolysaccharide O-polysaccharide antigen produced by Flavobacterium columnare ATCC 43622. Eur J Biochem 270:3440-3446. http://dx.doi.org/10.1046/j.1432-1033.2003.03736.x.
49. MacLean LL, Vinogradov E, Crump EM, Perry MB, Kay WW. 2001. The structure of the lipopolysaccharide O-antigen produced by Flavobacterium psychrophilum (259-93). Eur J Biochem 268:2710-2716. http://dx.doi.org/10.1046/j.1432-1327.2001.02163.x.
50. Qin QL, Zhang XY, Wang XM, Liu GM, Chen XL, Xie BB, Dang HY, Zhou BC, Yu J, Zhang YZ. 2010. The complete genome of Zunongwangia profunda SM-A87 reveals its adaptation to the deep-sea environment and ecological role in sedimentary organic nitrogen degradation. BMC Genomics 11:247. http://dx.doi.org/10.1186/1471-2164-11-247.
51. Rossolini GM, Franceschini N, Lauretti L, Caravelli B, Riccio ML, Galleni M, Frere JM, Amicosante G. 1999. Cloning of a Chryseobacterium (Flavobacterium) meningosepticum chromosomal gene (blaA(CME)) encoding an extended-spectrum class A beta-lactamase related to the Bacteroides cephalosporinases and the VEB-1 and PER beta-lactamases. Antimicrob Agents Chemother 43:2193-2199.
52. Schweizer HP. 2012. Mechanisms of antibiotic resistance in Burkholderia pseudomallei: implications for treatment of melioidosis. Future Microbiol 7:1389-1399. http://dx.doi.org/10.2217/fmb.12.116.
53. Kim C, Milheirico C, Gardete S, Holmes MA, Holden MT, de Lencastre H, Tomasz A. 2012. Properties of a novel PBP2A protein homolog from Staphylococcus aureus strain LGA251 and its contribution to the betalactam-resistant phenotype. J Biol Chem 287:36854-36863. http://dx.doi.org/10.1074/jbc.M112.395962.
54. Moran-Barrio J, Gonzalez JM, Lisa MN, Costello AL, Peraro MD, Carloni P, Bennett B, Tierney DL, Limansky AS, Viale AM, Vila AJ. 2007. The metallo-beta-lactamase GOB is a mono-Zn(II) enzyme with a novel active site. J Biol Chem 282:18286-18293. http://dx.doi.org/10.1074/jbc.M700467200.
55. Yum JH, Lee EY, Hur SH, Jeong SH, Lee H, Yong D, Chong Y, Lee EW, Nordmann P, Lee K. 2010. Genetic diversity of chromosomal metallobeta-lactamase genes in clinical isolates of Elizabethkingia meningoseptica from Korea. J Microbiol 48:358-364. http://dx.doi.org/10.1007/s12275-010-9308-5.
56. Bellais S, Poirel L, Leotard S, Naas T, Nordmann P. 2000. Genetic diversity of carbapenem-hydrolyzing metallo-beta-lactamases from Chryseobacterium (Flavobacterium) indologenes. Antimicrob Agents Chemother 44:3028-3034. http://dx.doi.org/10.1128/AAC.44.11.3028-3034.2000.
57. Glavina Del Rio T, Abt B, Spring S, Lapidus A, Nolan M, Tice H, Copeland A, Cheng JF, Chen F, Bruce D, Goodwin L, Pitluck S, Ivanova N, Mavromatis K, Mikhailova N, Pati A, Chen A, Palaniappan K, Land M, Hauser L, Chang YJ, Jeffries CD, Chain P, Saunders E, Detter JC, Brettin T, Rohde M, Goker M, Bristow J, Eisen JA, Markowitz V, Hugenholtz P, Kyrpides NC, Klenk HP, Lucas S. 2010. Complete genome sequence of Chitinophaga pinensis type strain (UQM 2034). Stand Genomic Sci 2:87-95. http://dx.doi.org/10.4056/sigs.661199.
58. Biswas S, Brunel JM, Dubus JC, Reynaud-Gaubert M, Rolain JM. 2012. Colistin: an update on the antibiotic of the 21st century. Expert Rev Anti Infect Ther 10:917-934. http://dx.doi.org/10.1586/eri.12.78.
59. Landman D, Georgescu C, Martin DA, Quale J. 2008. Polymyxins revisited. Clin Microbiol Rev 21:449-465. http://dx.doi.org/10.1128/CMR.00006-08.
60. Hamad MA, Di Lorenzo F, Molinaro A, Valvano MA. 2012. Aminoarabinose is essential for lipopolysaccharide export and intrinsic antimicrobial peptide resistance in Burkholderia cenocepacia. Mol Microbiol 85: 962-974. http://dx.doi.org/10.1111/j.1365-2958.2012.08154.x.
61. Campos MA, Vargas MA, Regueiro V, Llompart CM, Alberti S, Bengoechea JA. 2004. Capsule polysaccharide mediates bacterial resistance to antimicrobial peptides. Infect Immun 72:7107-7114. http://dx.doi.org/10.1128/IAI.72.12.7107-7114.2004.
62. Diene SM, Merhej V, Henry M, El FA, Roux V, Robert C, Azza S, Gavory F, Barbe V, La SB, Raoult D, Rolain JM. 2013. The rhizome of the multidrug-resistant Enterobacter aerogenes genome reveals how new "killer bugs" are created because of a sympatric lifestyle. Mol Biol Evol 30:369-383. http://dx.doi.org/10.1093/molbev/mss236.
63. Rolain JM, Diene SM, Kempf M, Gimenez G, Robert C, Raoult D. 2013. Real-time sequencing to decipher the molecular mechanism of resistance of a clinical pan-drug-resistant Acinetobacter baumannii isolate from Marseille, France. Antimicrob Agents Chemother 57:592-596. http://dx.doi.org/10.1128/AAC.01314-12.
64. Arroyo LA, Herrera CM, Fernandez L, Hankins JV, Trent MS, Hancock RE. 2011. The pmrCAB operon mediates polymyxin resistance in Acinetobacter baumannii ATCC 17978 and clinical isolates through phosphoethanolamine modification of lipid A. Antimicrob Agents Chemother 55: 3743-3751. http://dx.doi.org/10.1128/AAC.00256-11.
65. Moffatt JH, Harper M, Harrison P, Hale JD, Vinogradov E, Seemann T, Henry R, Crane B, St. Michael F, Cox AD, Adler B, Nation RL, Li J, Boyce JD. 2010. Colistin resistance in Acinetobacter baumannii is mediated by complete loss of lipopolysaccharide production. Antimicrob Agents Chemother 54:4971-4977. http://dx.doi.org/10.1128/AAC.00834-10.
66. Moffatt JH, Harper M, Adler B, Nation RL, Li J, Boyce JD. 2011. Insertion sequence ISAba11 is involved in colistin resistance and loss of lipopolysaccharide in Acinetobacter baumannii. Antimicrob Agents Chemother 55:3022-3024. http://dx.doi.org/10.1128/AAC.01732-10.
67. Clements JM, Coignard F, Johnson I, Chandler S, Palan S, Waller A, Wijkmans J, Hunter MG. 2002. Antibacterial activities and characterization of novel inhibitors of LpxC. Antimicrob Agents Chemother 46:1793-1799. http://dx.doi.org/10.1128/AAC.46.6.1793-1799.2002.
68. Bartling CM, Raetz CR. 2009. Crystal structure and acyl chain selectivity of Escherichia coli LpxD, the N-acyltransferase of lipid A biosynthesis. Biochemistry 48:8672-8683. http://dx.doi.org/10.1021/bi901025v.