메뉴 건너뛰기




Volumn 9, Issue 10, 2014, Pages 2382-2394

Pre-embedding immunogold labeling to optimize protein localization at subcellular compartments and membrane microdomains of leukocytes

Author keywords

[No Author keywords available]

Indexed keywords

RESIN; PROTEIN;

EID: 84923138765     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2014.163     Document Type: Article
Times cited : (60)

References (34)
  • 1
    • 38449104405 scopus 로고    scopus 로고
    • Processing tissue and cells for transmission electron microscopy in diagnostic pathology and research
    • Graham, L. & Orenstein, J.M. Processing tissue and cells for transmission electron microscopy in diagnostic pathology and research. Nat. Protoc. 2, 2439-2450 (2007).
    • (2007) Nat. Protoc. , vol.2 , pp. 2439-2450
    • Graham, L.1    Orenstein, J.M.2
  • 2
    • 0041837558 scopus 로고    scopus 로고
    • Electron microscopy in cell biology: Integrating structure and function
    • Koster, A.J. & Klumperman, J. Electron microscopy in cell biology: integrating structure and function. Nat. Rev. Mol. Cell Biol. (suppl.): SS6-SS10 (2003).
    • (2003) Nat. Rev. Mol. Cell Biol. , pp. SS6-SS10
    • Koster, A.J.1    Klumperman, J.2
  • 3
    • 84885390620 scopus 로고    scopus 로고
    • Studying synapses in human brain with array tomography and electron microscopy
    • Kay, K.R. et al. Studying synapses in human brain with array tomography and electron microscopy. Nat. Protoc. 8, 1366-1380 (2013).
    • (2013) Nat. Protoc. , vol.8 , pp. 1366-1380
    • Kay, K.R.1
  • 4
    • 84881023889 scopus 로고    scopus 로고
    • Insights into cell entry and intracellular trafficking of peptide and protein drugs provided by electron microscopy
    • Margus, H., Padari, K. & Pooga, M. Insights into cell entry and intracellular trafficking of peptide and protein drugs provided by electron microscopy. Adv. Drug Deliv. Rev. 65, 1031-1038 (2013).
    • (2013) Adv. Drug Deliv. Rev. , vol.65 , pp. 1031-1038
    • Margus, H.1    Padari, K.2    Pooga, M.3
  • 6
    • 84864386738 scopus 로고    scopus 로고
    • Correlative light and electron microscopy in parasite research
    • Loussert, C., Forestier, C.L. & Humbel, B.M. Correlative light and electron microscopy in parasite research. Methods Cell Biol. 111, 59-73 (2012).
    • (2012) Methods Cell Biol. , vol.111 , pp. 59-73
    • Loussert, C.1    Forestier, C.L.2    Humbel, B.M.3
  • 7
    • 78049525941 scopus 로고    scopus 로고
    • Pre-embedding immunogold localization of antigens in mammalian brain slices
    • Schikorski, T. Pre-embedding immunogold localization of antigens in mammalian brain slices. Methods Mol. Biol. 657, 133-144 (2010).
    • (2010) Methods Mol. Biol. , vol.657 , pp. 133-144
    • Schikorski, T.1
  • 8
    • 0031879396 scopus 로고    scopus 로고
    • Preparation, use, and enlargement of ultrasmall gold particles in immunoelectron microscopy
    • Baschong, W. & Stierhof, Y.D. Preparation, use, and enlargement of ultrasmall gold particles in immunoelectron microscopy. Microsc. Res. Tech. 42, 66-79 (1998).
    • (1998) Microsc. Res. Tech. , vol.42 , pp. 66-79
    • Baschong, W.1    Stierhof, Y.D.2
  • 9
    • 0018082456 scopus 로고
    • Ultrastructural localization of intracellular antigens by the use of protein A-gold complex
    • Roth, J., Bendayan, M. & Orci, L. Ultrastructural localization of intracellular antigens by the use of protein A-gold complex. J. Histochem. Cytochem. 26, 1074-1081 (1978).
    • (1978) J. Histochem. Cytochem. , vol.26 , pp. 1074-1081
    • Roth, J.1    Bendayan, M.2    Orci, L.3
  • 10
    • 0034037769 scopus 로고    scopus 로고
    • Ultrastructural localization of the vascular permeability factor/vascular endothelial growth factor (VPF/VEGF) receptor-2 (FLK-1, KDR) in normal mouse kidney and in the hyperpermeable vessels induced by VPF/VEGF-expressing tumors and adenoviral vectors
    • Feng, D. et al. Ultrastructural localization of the vascular permeability factor/vascular endothelial growth factor (VPF/VEGF) receptor-2 (FLK-1, KDR) in normal mouse kidney and in the hyperpermeable vessels induced by VPF/VEGF-expressing tumors and adenoviral vectors. J. Histochem. Cytochem. 48, 545-556 (2000).
    • (2000) J. Histochem. Cytochem. , vol.48 , pp. 545-556
    • Feng, D.1
  • 11
    • 73549102459 scopus 로고    scopus 로고
    • An initial step of GAS-containing autophagosome-like vacuoles formation requires Rab7
    • Yamaguchi, H. et al. An initial step of GAS-containing autophagosome-like vacuoles formation requires Rab7. PLoS Pathog. 5, e1000670 (2009).
    • (2009) PLoS Pathog. , vol.5 , pp. e1000670
    • Yamaguchi, H.1
  • 12
    • 84875291355 scopus 로고    scopus 로고
    • Relation between ultrastructural localization, changes in caveolin-1, and capillarization of liver sinusoidal endothelial cells in human hepatitis C-related cirrhotic liver
    • Yamazaki, H. et al. Relation between ultrastructural localization, changes in caveolin-1, and capillarization of liver sinusoidal endothelial cells in human hepatitis C-related cirrhotic liver. J. Histochem. Cytochem. 61, 169-176 (2013).
    • (2013) J. Histochem. Cytochem. , vol.61 , pp. 169-176
    • Yamazaki, H.1
  • 13
    • 78049500404 scopus 로고    scopus 로고
    • Pre-embedding Nanogold silver and gold intensification
    • Yamamoto, A. & Masaki, R. Pre-embedding Nanogold silver and gold intensification. Methods Mol. Biol. 657, 225-235 (2010).
    • (2010) Methods Mol. Biol. , vol.657 , pp. 225-235
    • Yamamoto, A.1    Masaki, R.2
  • 14
    • 0028036932 scopus 로고
    • Prostaglandin endoperoxide synthase (cyclooxygenase): Ultrastructural localization to nonmembrane-bound cytoplasmic lipid bodies in human eosinophils and 3T3 fibroblasts
    • Dvorak, A.M., Morgan, E.S., Tzizik, D.M. & Weller, P.F. Prostaglandin endoperoxide synthase (cyclooxygenase): ultrastructural localization to nonmembrane-bound cytoplasmic lipid bodies in human eosinophils and 3T3 fibroblasts. Int. Arch. Allergy Immunol. 105, 245-250 (1994).
    • (1994) Int. Arch. Allergy Immunol. , vol.105 , pp. 245-250
    • Dvorak, A.M.1    Morgan, E.S.2    Tzizik, D.M.3    Weller, P.F.4
  • 15
    • 0031715571 scopus 로고    scopus 로고
    • Pathways for eosinophil lipid body induction: Differing signal transduction in cells from normal and hypereosinophilic subjects
    • Bozza, P.T., Yu, W., Cassara, J. & Weller, P.F. Pathways for eosinophil lipid body induction: differing signal transduction in cells from normal and hypereosinophilic subjects. J. Leukoc. Biol. 64, 563-569 (1998).
    • (1998) J. Leukoc. Biol. , vol.64 , pp. 563-569
    • Bozza, P.T.1    Yu, W.2    Cassara, J.3    Weller, P.F.4
  • 16
    • 78049522481 scopus 로고    scopus 로고
    • Pre-embedding immunoelectron microscopy of chemically fixed mammalian tissue culture cells
    • Hagiwara, H., Aoki, T., Suzuki, T. & Takata, K. Pre-embedding immunoelectron microscopy of chemically fixed mammalian tissue culture cells. Methods Mol. Biol. 657, 145-154 (2010).
    • (2010) Methods Mol. Biol. , vol.657 , pp. 145-154
    • Hagiwara, H.1    Aoki, T.2    Suzuki, T.3    Takata, K.4
  • 17
    • 67649880314 scopus 로고    scopus 로고
    • Vesicle-mediated secretion of human eosinophil granule-derived major basic protein
    • Melo, R.C.N. et al. Vesicle-mediated secretion of human eosinophil granule-derived major basic protein. Lab. Invest. 89, 769-781 (2009).
    • (2009) Lab. Invest. , vol.89 , pp. 769-781
    • Melo, R.C.N.1
  • 18
    • 0026536069 scopus 로고
    • A 1.4-nm gold cluster covalently attached to antibodies improves immunolabeling
    • Hainfeld, J.F. & Furuya, F.R. A 1.4-nm gold cluster covalently attached to antibodies improves immunolabeling. J. Histochem. Cytochem. 40, 177-184 (1992).
    • (1992) J. Histochem. Cytochem. , vol.40 , pp. 177-184
    • Hainfeld, J.F.1    Furuya, F.R.2
  • 19
    • 84871482550 scopus 로고    scopus 로고
    • Eosinophils: Changing perspectives in health and disease
    • Rosenberg, H.F., Dyer, K.D. & Foster, P.S. Eosinophils: changing perspectives in health and disease. Nat. Rev. Immunol. 13, 9-22 (2013).
    • (2013) Nat. Rev. Immunol. , vol.13 , pp. 9-22
    • Rosenberg, H.F.1    Dyer, K.D.2    Foster, P.S.3
  • 20
    • 26944471127 scopus 로고    scopus 로고
    • Human eosinophils secrete preformed, granule-stored interleukin-4 through distinct vesicular compartments
    • Melo, R.C.N. et al. Human eosinophils secrete preformed, granule-stored interleukin-4 through distinct vesicular compartments. Traffic 6, 1047-1057 (2005).
    • (2005) Traffic , vol.6 , pp. 1047-1057
    • Melo, R.C.N.1
  • 21
    • 33644765838 scopus 로고    scopus 로고
    • Cytokine receptor-mediated trafficking of preformed IL-4 in eosinophils identifies an innate immune mechanism of cytokine secretion
    • Spencer, L.A. et al. Cytokine receptor-mediated trafficking of preformed IL-4 in eosinophils identifies an innate immune mechanism of cytokine secretion. Proc. Natl. Acad. Sci. USA 103, 3333-3338 (2006).
    • (2006) Proc. Natl. Acad. Sci. USA , vol.103 , pp. 3333-3338
    • Spencer, L.A.1
  • 22
    • 63849314570 scopus 로고    scopus 로고
    • Mature human eosinophils express functional Notch ligands mediating eosinophil autocrine regulation
    • Radke, A.L. et al. Mature human eosinophils express functional Notch ligands mediating eosinophil autocrine regulation. Blood 113, 3092-3101 (2009).
    • (2009) Blood , vol.113 , pp. 3092-3101
    • Radke, A.L.1
  • 23
    • 24944528428 scopus 로고    scopus 로고
    • Intragranular vesiculotubular compartments are involved in piecemeal degranulation by activated human eosinophils
    • Melo, R.C.N., Perez, S.A., Spencer, L.A., Dvorak, A.M. & Weller, P.F. Intragranular vesiculotubular compartments are involved in piecemeal degranulation by activated human eosinophils. Traffic 6, 866-879 (2005).
    • (2005) Traffic , vol.6 , pp. 866-879
    • Melo, R.C.N.1    Perez, S.A.2    Spencer, L.A.3    Dvorak, A.M.4    Weller, P.F.5
  • 24
    • 84856683094 scopus 로고    scopus 로고
    • MHC class II and CD9 in human eosinophils localize to detergent-resistant membrane microdomains
    • Akuthota, P., Melo, R.C.N., Spencer, L.A. & Weller, P.F. MHC class II and CD9 in human eosinophils localize to detergent-resistant membrane microdomains. Am. J. Respir. Cell Mol. Biol. 46, 188-195 (2012).
    • (2012) Am. J. Respir. Cell Mol. Biol. , vol.46 , pp. 188-195
    • Akuthota, P.1    Melo, R.C.N.2    Spencer, L.A.3    Weller, P.F.4
  • 25
    • 49549087335 scopus 로고    scopus 로고
    • Electron tomography and immunonanogold electron microscopy for investigating intracellular trafficking and secretion in human eosinophils
    • Melo, R.C.N., Dvorak, A.M. & Weller, P.F. Electron tomography and immunonanogold electron microscopy for investigating intracellular trafficking and secretion in human eosinophils. J. Cell Mol. Med. 12, 1416-1419 (2008).
    • (2008) J. Cell Mol. Med. , vol.12 , pp. 1416-1419
    • Melo, R.C.N.1    Dvorak, A.M.2    Weller, P.F.3
  • 26
    • 38949090309 scopus 로고    scopus 로고
    • Mechanisms of eosinophil secretion: Large vesiculotubular carriers mediate transport and release of granule-derived cytokines and other proteins
    • Melo, R.C.N., Spencer, L.A., Dvorak, A.M. & Weller, P.F. Mechanisms of eosinophil secretion: large vesiculotubular carriers mediate transport and release of granule-derived cytokines and other proteins. J. Leukoc. Biol. 83, 229-236 (2008).
    • (2008) J. Leukoc. Biol. , vol.83 , pp. 229-236
    • Melo, R.C.N.1    Spencer, L.A.2    Dvorak, A.M.3    Weller, P.F.4
  • 27
    • 84901413350 scopus 로고    scopus 로고
    • Human eosinophil leukocytes express protein disulfide isomerase in secretory granules and vesicles: Ultrastructural studies
    • Dias, F.F. et al. Human eosinophil leukocytes express protein disulfide isomerase in secretory granules and vesicles: ultrastructural studies. J. Histochem. Cytochem. 62, 450-459 (2014).
    • (2014) J. Histochem. Cytochem. , vol.62 , pp. 450-459
    • Dias, F.F.1
  • 29
    • 0034085885 scopus 로고    scopus 로고
    • A practical technique to postfix Nanogold-immunolabeled specimens with osmium and to embed them in Epon for electron microscopy
    • Sawada, H. & Esaki, M. A practical technique to postfix Nanogold-immunolabeled specimens with osmium and to embed them in Epon for electron microscopy. J. Histochem. Cytochem. 48, 493-498 (2000).
    • (2000) J. Histochem Cytochem. , vol.48 , pp. 493-498
    • Sawada, H.1    Esaki, M.2
  • 30
    • 0033994538 scopus 로고    scopus 로고
    • The mouse SKD1, a homologue of yeast Vps4p, is required for normal endosomal trafficking and morphology in mammalian cells
    • Yoshimori, T. et al. The mouse SKD1, a homologue of yeast Vps4p, is required for normal endosomal trafficking and morphology in mammalian cells. Mol. Biol. Cell 11, 747-763 (2000).
    • (2000) Mol. Biol. Cell , vol.11 , pp. 747-763
    • Yoshimori, T.1
  • 31
    • 79955932942 scopus 로고    scopus 로고
    • Multiple-labelling immunoEM using different sizes of colloidal gold: Alternative approaches to test for differential distribution and colocalization in subcellular structures
    • Mayhew, T.M. & Lucocq, J.M. Multiple-labelling immunoEM using different sizes of colloidal gold: alternative approaches to test for differential distribution and colocalization in subcellular structures. Histochem. Cell Biol. 135, 317-326 (2011).
    • (2011) Histochem. Cell Biol. , vol.135 , pp. 317-326
    • Mayhew, T.M.1    Lucocq, J.M.2
  • 32
    • 79451470428 scopus 로고    scopus 로고
    • Piecemeal degranulation in human eosinophils: A distinct secretion mechanism underlying inflammatory responses
    • Melo, R.C.N. & Weller, P.F. Piecemeal degranulation in human eosinophils: a distinct secretion mechanism underlying inflammatory responses. Histol. Histopathol. 25, 1341-1354 (2010).
    • (2010) Histol. Histopathol. , vol.25 , pp. 1341-1354
    • Melo, R.C.N.1    Weller, P.F.2
  • 33
    • 79451475122 scopus 로고    scopus 로고
    • Contributions of electron microscopy to understand secretion of immune mediators by human eosinophils
    • Melo, R.C.N., Dvorak, A.M. & Weller, P.F. Contributions of electron microscopy to understand secretion of immune mediators by human eosinophils. Microsc. Microanal. 16, 653-660 (2010).
    • (2010) Microsc. Microanal. , vol.16 , pp. 653-660
    • Melo, R.C.N.1    Dvorak, A.M.2    Weller, P.F.3
  • 34
    • 55749099013 scopus 로고    scopus 로고
    • Neutrophils recruited to the site of Mycobacterium bovis BCG infection undergo apoptosis and modulate lipid body biogenesis and prostaglandin e production by macrophages
    • D'Avila, H. et al. Neutrophils recruited to the site of Mycobacterium bovis BCG infection undergo apoptosis and modulate lipid body biogenesis and prostaglandin E production by macrophages. Cell Microbiol. 10, 2589-2604 (2008).
    • (2008) Cell Microbiol. , vol.10 , pp. 2589-2604
    • D'avila, H.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.