Pre-embedding immunogold localization of antigens in mammalian brain slices

Methods in Molecular Biology

Volumn 657, Issue , 2010, Pages 133-144

  Schikorski, Thomas ^a  

^a SCHOOL OF MEDICINE   (Puerto Rico)

Author keywords

Correlated light and electron microscopy; Electron microscopy; Fluoro nanogold; Gold enhancement; Immunohistochemistry; Permeabilization; Synaptic vesicle protein

Indexed keywords

MAMMALIA;

ANTIBODY; ANTIGEN; COLLOIDAL GOLD; METAL NANOPARTICLE;

ANIMAL; ARTICLE; BRAIN; BRAIN LEVEL; CHEMISTRY; HISTOLOGY; IMMUNOHISTOCHEMISTRY; IMMUNOLOGY; METHODOLOGY; MOUSE; ULTRASTRUCTURE;

ANIMALS; ANTIBODIES; ANTIGENS; BRAIN; BRAIN CHEMISTRY; GOLD COLLOID; IMMUNOHISTOCHEMISTRY; METAL NANOPARTICLES; MICE; TISSUE EMBEDDING;

EID: 78049525941     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60761-783-9_10     Document Type: Article

References (4)

1. Hainfeld, J. F. and Furuya, F. R. (1992) A 1.4-nm gold cluster covalently attached to antibodies improves immunolabeling. J. Histochem. Cytochem. 40, 177-184.
2. Newman, G. R. and Hobot, J. R. (2001) Resin Microscopy and On-Section Immunocytochemistry. Springer Verlag, Berlin.
3. Karnovsky, M. J. (1965) A formaldehydeglutaraldehyde fixative of high osmolarity for use in electron microscopy. J. Cell. Biol. 27, 137A.
4. Hanaichi, T., Sato, T., Iwamato, T., Malavasi-Yamashiro, J., Hoshino, M., and Mizuno, M. (1986) A stable lead by modification of Sato's method. J. Electron Microsc. 35, 304-306. (Pubitemid 17065982)