Pre-embedding immunoelectron microscopy of chemically fixed mammalian tissue culture cells

Methods in Molecular Biology

Volumn 657, Issue , 2010, Pages 145-154

  Hagiwara, Haruo ^a   Aoki, Takeo ^a   Suzuki, Takeshi ^a   Takata, Kuniaki ^a  

^a GUNMA UNIVERSITY   (Japan)

Author keywords

Cultured cells; Nanogold; Pre embedding method; Silver enhancement

Indexed keywords

MAMMALIA;

FIXATIVE; FORMALDEHYDE; GOLD; IMMUNOGLOBULIN F(AB) FRAGMENT; METAL NANOPARTICLE; PARAFORMALDEHYDE; POLYMER; SILVER;

ARTICLE; CELL CULTURE; CHEMISTRY; CYTOLOGY; EUKARYOTIC FLAGELLUM; HISTOLOGY; HUMAN; IMMUNOELECTRON MICROSCOPY; KIDNEY; METHODOLOGY; PARTICLE SIZE; TISSUE FIXATION; ULTRASTRUCTURE;

CELLS, CULTURED; CILIA; FIXATIVES; FORMALDEHYDE; GOLD; HUMANS; IMMUNOGLOBULIN FAB FRAGMENTS; KIDNEY; METAL NANOPARTICLES; MICROSCOPY, IMMUNOELECTRON; PARTICLE SIZE; POLYMERS; SILVER; TISSUE EMBEDDING; TISSUE FIXATION;

EID: 78049522481     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60761-783-9_11     Document Type: Article

References (8)

1. Hagiwara, H., Aoki, T., Kano, T., and Ohwada, N. (2000). Immunocytochemistry of the striated rootlets associated with solitary cilia in human oviductal secretory cells. Histochem. Cell Biol. 114, 205-212.
2. Ferguson, D. J., Hughes, D. A., and Beesley, J. E. (1998) Immunogold probes in electron microscopy. Methods Mol. Biol. 80, 297-311.
3. Hainfeld, J. F. and Furuya, F. R. (1992) A 1.4-nm gold cluster covalently attached to antibodies improves immunolabeling. J. Histochem. Cytochem. 40, 177-184.
4. Mizoguchi, A., Nakanishi, H., Kimura, K., Matsubara, K., Ozaki-Kuroda, K., Katata, T., Honda, T., Kiyohara, Y., Heo, K., Higashi, M., Tsutsumi, T., Sonoda, S., Ide, C., and Takai, Y. (2002) Nectin: an adhesion molecule involved in formation of synapses. J. Cell Biol. 156, 555-565. (Pubitemid 34839902)
5. Shiba, D., Yamaoka, Y., Hagiwara, H., Takamatsu, T., Hamada, H., and Yokoyama, T. (2009) Localization of Inv in a distinctive intraciliary compartment requires the Cterminal ninein-homolog-containing region. J. Cell Sci. 122, 44-54.
6. Humbel, B. M., Sibon, O. C., Stierhof, Y. D., and Schwarz, H. (1995) Ultra-small gold particles and silver enhancement as a detection system in immunolabeling and in situ hybridization experiments. J. Histochem. Cytochem. 43, 735-737.
7. Harlow, E. and Lane, D. (1999) Using Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, USA.
8. Reynolds, E. S. (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208-212.