Organic substances interfere with reverse transcription-quantitative PCR-based virus detection in water samples

Applied and Environmental Microbiology

Volumn 81, Issue 5, 2015, Pages 1585-1593

  Hata, Akihiko ^a   Katayama, Hiroyuki ^b   Furumai, Hiroaki ^b  

^a KYOTO UNIVERSITY   (Japan)

^b UNIVERSITY OF TOKYO   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL MATERIALS; ORGANIC ACIDS; POLYMERASE CHAIN REACTION; RIVERS; TRANSCRIPTION; VIRUSES; WATER RESOURCES;

EXCITATION EMISSION MATRICES; ORGANIC FRACTIONS; ORGANIC PROPERTIES; ORGANIC SUBSTANCES; REVERSE TRANSCRIPTION; REVERSE TRANSCRIPTION QUANTITATIVE-PCR; RIVER WATER SAMPLES; VIRUS CONCENTRATION;

DRIERS (MATERIALS);

ADSORPTION; CONCENTRATION (COMPOSITION); DETECTION METHOD; HUMIC ACID; INHIBITION; ORGANIC COMPOUND; POLYMERASE CHAIN REACTION; PROTEIN; RIVER WATER; VIRUS;

POLIOVIRUS;

ENZYME INHIBITOR; ORGANIC COMPOUND;

ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR DIAGNOSIS; PROCEDURES; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VIROLOGY; VIRUS;

ENZYME INHIBITORS; MOLECULAR DIAGNOSTIC TECHNIQUES; ORGANIC CHEMICALS; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VIROLOGY; VIRUSES;

EID: 84922876606     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.03082-14     Document Type: Article

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