Chromatographic adsorption of serum albumin and antibody proteins in cryogels with benzyl-quaternary amine ligands

Journal of Chromatography A

Volumn 1381, Issue , 2015, Pages 173-183

  Yun, Junxian ^a   Cheng, Xiuhong ^a   Ye, Jialei ^a   Shen, Shaochuan ^a   Yang, Gensheng ^a   Yao, Kejian ^a   Kirsebom, Harald ^b   Lin, Dong Qiang ^c   Guan, Yi Xin ^c   Yao, Shan Jing ^c  

^a ZHEJIANG UNIVERSITY OF TECHNOLOGY   (China)

^b LUND UNIVERSITY   (Sweden)

^c ZHEJIANG UNIVERSITY   (China)

Author keywords

Adsorption; Bioseparation; Chromatography; Cryogel; Mathematical modeling

Indexed keywords

ADSORPTION; ANTIBODIES; BIOCHEMISTRY; HYDROPHOBICITY; ION EXCHANGE; MAMMALS; MASS TRANSFER; MATHEMATICAL MODELS;

BIO-SEPARATION; BIOACTIVE PROTEINS; CHROMATOGRAPHIC ISOLATION; CRYOGELS; EQUILIBRIUM ADSORPTION ISOTHERMS; HYDROPHOBIC INTERACTIONS; PROTEIN ADSORPTION; PROTEIN ISOELECTRIC POINTS;

CHROMATOGRAPHY;

ADSORBENT; AMMONIUM CHLORIDE; BOVINE SERUM ALBUMIN; IMMUNOGLOBULIN G ANTIBODY; POLYACRYLAMIDE; SERUM ALBUMIN; UNCLASSIFIED DRUG; VINYLBENZYL TRIMETHYLAMMONIUM CHLORIDE; ACRYLIC ACID RESIN; CRYOGEL; IMMUNOGLOBULIN G; LIGAND; POLY(VINYLBENZYLTRIMETHYLAMMONIUM CHLORIDE); POLYSTYRENE DERIVATIVE; QUATERNARY AMMONIUM DERIVATIVE;

ADSORPTION KINETICS; ARTICLE; BIOSEPARATION; CHROMATOGRAPHY; CONTROLLED STUDY; CRYOGEL; HYDROPHOBICITY; ION EXCHANGE; ISOELECTRIC POINT; NONHUMAN; PH; POLYMERIZATION; PROTEIN BINDING; PROTEIN ISOLATION; PROTEIN PURIFICATION; RABBIT; SEPARATION TECHNIQUE; ADSORPTION; ANIMAL; BOVINE; CHEMICAL PHENOMENA; CHEMISTRY; LIQUID CHROMATOGRAPHY; MOLECULAR WEIGHT; PROCEDURES; SYNTHESIS;

BOVINAE; ORYCTOLAGUS CUNICULUS;

ACRYLIC RESINS; ADSORPTION; ANIMALS; CATTLE; CHROMATOGRAPHY, LIQUID; CRYOGELS; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; IMMUNOGLOBULIN G; LIGANDS; MOLECULAR WEIGHT; POLYSTYRENES; QUATERNARY AMMONIUM COMPOUNDS; RABBITS; SERUM ALBUMIN;

EID: 84922791871     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2014.11.081     Document Type: Article

References (78)

1. Mattiasson B., Kumar A., Galaev I.Y. Macroporous Polymers: Production Properties and Biotechnological/Biomedical Applications 2010, CRC Press, Taylor & Francis Group, Boca Raton, USA.
2. Kirsebom H., Mattiasson B. Cryostructuration as a tool for preparing highly porous polymer materials. Polym. Chem. 2011, 2:1059-1062.
3. Plieva F.M., Kirsebom H., Mattiasson B. Preparation of macroporous cryostructurated gel monoliths, their characterization and main applications. J. Sep. Sci. 2011, 34:2164-2172.
4. Plieva F.M., Galaev I.Y., Noppe W., Mattiasson B. Cryogel applications in microbiology. Trends Microbiol. 2008, 16:543-551.
5. Lozinsky V.I. Polymeric cryogels as a new family of macroporous and supermacroporous materials for biotechnological purposes. Russ. Chem. Bull. 2008, 57:1015-1032.
6. Plieva F.M., Galaev I.Y., Mattiasson B. Macroporous gels prepared at subzero temperatures as novel materials for chromatography of particulate-containing fluids and cell culture applications. J. Sep. Sci. 2007, 30:1657-1671.
7. Lozinsky V.I., Galaev I.Y., Plieva F.M., Savina I.N., Jungvid H., Mattiasson B. Polymeric cryogels as promising materials of biotechnological interest. Trends Biotechnol. 2003, 21:445-451.
8. Lozinsky V.I. Cryogels on the basis of natural and synthetic polymers: preparation, properties and applications. Russ. Chem. Rev. 2002, 71:489-511.
9. Lozinsky V.I., Plieva F.M., Galaev I.Y., Mattiasson B. The potential of polymeric cryogels in bioseparation. Bioseparation 2002, 10:163-188.
10. Gun'ko V.M., Savina I.N., Mikhalovsky S.V. Cryogels: morphological, structural and adsorption characterization. Adv. Colloid Interfac. 2013, 187-188:1-46.
11. Arvidsson P., Plieva F.M., Lozinsky V.I., Galaev I.Y., Mattiasson B. Direct chromatographic capture of enzyme from crude homogenate using immobilized metal affinity chromatography on a continuous supermacroporous adsorbent. J. Chromatogr. A 2003, 986:275-290.
12. Arvidsson P., Plieva F.M., Savina I.N., Lozinsky V.I., Fexby S., Bulow L., Galaev I.Y., Mattiasson B. Chromatography of microbial cells using continuous supermacroporous affinity and ion-exchange columns. J. Chromatogr. A 2002, 977:27-38.
13. Bibi N.S., Singh N.K., Dsouza R.N., Aasim M., Fernández-Lahore M. Synthesis and performance of megaporous immobilized metal-ion affinity cryogels for recombinant protein capture and purification. J. Chromatogr. A 2013, 1272:145-149.
14. Chen F., Yao K.J., Yun J.X., Shen S.C. Influence of grafting conditions on the properties of polyacrylamide-based cation-exchange cryogels grafted with 2-acrylamido-2-methyl-1 -propanesulfonic acid. Chem. Eng. Sci. 2008, 63:71-77.
15. Chen Y., Shen S.C., Yun J.X., Yao K.J. Isolation of ATP from a yeast fermentation broth using a cryogel column at high flow velocities. J. Sep. Sci. 2008, 31:3879-3883.
16. Dainiak M.B., Kumara A., Plieva F.M., Galaev I.Y., Mattiasson B. Integrated isolation of antibody fragments from microbial cell culture fluids using supermacroporous cryogels. J. Chromatogr. A 2004, 1045:93-98.
17. Deraz S., Plieva F.M., Galaev I.Y., Karlsson E.N., Mattiasson B. Capture of bacteriocins directly from non-clarified fermentation broth using macroporous monolithic cryogels with phenyl ligands. Enzyme Microb. Technol. 2007, 40:786-793.
18. Hajizadeh S., Kirsebom H., Leistner A., Mattiasson B. Composite cryogel with immobilized concanavalin A for affinity chromatography of glycoproteins. J. Sep. Sci. 2012, 35:2978-2985.
19. Hanora A., Plieva F.M., Hedström M., Galaev I.Y., Mattiasson B. Capture of bacterial endotoxins using a supermacroporous monolithic matrix with immobilized polyethyleneimine, lysozyme or polymyxin B. J. Biotechnol. 2005, 118:421-433.
20. Hanora A., Savina I.N., Plieva F.M., Izumrudov V.A., Mattiasson B., Galaev I.Y. Direct capture of plasmid DNA from non-clarified bacterial lysate using polycation-grafted monoliths. J. Biotechnol. 2006, 123:343-355.
21. Kirsebom H., Rata G., Topgaard D., Mattiasson B., Galaev I.Y. In situ 1H NMR studies of free radical cryopolymerization. Polymer 2008, 49:3855-3858.
22. Kumar A., Bansal V., Nandakumar K.S., Galaev I.Y., Roychoudhury P.K., Holmdah R., Mattiasson B. Integrated bioprocess for the production and isolation of urokinase from animal cell culture using supermacroporous cryogel matrices. Biotechnol. Bioeng. 2006, 93:636-646.
23. Plieva F.M., Bober B., Dainiak M., Galaev I.Y., Mattiasson B. Macroporous polyacrylamide monolithic gels with immobilized metal affinity ligands: the effect of porous structure and ligand coupling chemistry on protein binding. J. Mol. Recogn. 2006, 19:305-312.
24. Savina I.N., Galaev I.Y., Mattiasson B. Anion-exchange supermacroporous monolithic matrices with grafted polymer brushes of N,N-dimethylaminoethyl-methacrylate. J. Chromatogr. A 2005, 1092:199-205.
25. Savina I.N., Mattiasson B., Galaev I.Y. Graft polymerization of acrylic acid onto macroporous polyacrylamide gel (cryogel) initiated by potassium diperiodatocuprate. Polymer 2005, 46:9596-9603.
26. Savina I.N., Galaev I.Y., Mattiasson B. Ion-exchange macroporous hydrophilic gel monolith with grafted polymer brushes. J. Mol. Recogn. 2006, 19:313-321.
27. Savina I.N., Mattiasson B., Galaev I.Y. Graft polymerization of vinyl monomers inside macroporous polyacrylamide gel, cryogel, in aqueous and aqueous-organic media initiated by diperiodatocuprate(III) complexes. J. Polym. Sci. Part A: Polym. Chem. 2006, 44:1952-1963.
28. Yao K.J., Shen S.C., Yun J.X., Wang L.H., Chen F., Yu X.M. Protein adsorption in supermacroporous cryogels with embedded nanoparticles. Biochem. Eng. J. 2007, 36:139-146.
29. Yao K.J., Yun J.X., Shen S.C., Chen F. In-situ graft-polymerization preparation of cation-exchange supermacroporous cryogel with sulfo groups in glass columns. J. Chromatogr. A 2007, 1157:246-251.
30. Yao K.J., Shen S.C., Yun J.X., Wang L.H., He X.J., Yu X.M. Preparation of polyacrylamide-based supermacroporous monolithic cryogel beds under freezing-temperature variation conditions. Chem. Eng. Sci. 2006, 61:6701-6708.
31. Yao K.J., Yun J.X., Shen S.C., Wang L.H., He X.J., Yu X.M. Characterization of a novel continuous supermacroporous monolithic cryogel embedded with nanoparticles for protein chromatography. J. Chromatogr. A 2006, 1109:103-110.
32. Wang L.H., Shen S.C., Yun J.X., Yao K.J., Yao S.J. Chromatographic separation of cytidine triphosphate from fermentation broth of yeast using anion-exchange cryogel. J. Sep. Sci. 2008, 31:689-695.
33. Yan L.D., Shen S.C., Yun J.X., Yao K.J. Isolation of lysozyme from chicken egg white using polyacrylamide-based cation-exchange cryogel. Chin. J. Chem. Eng. 2011, 19:876-880.
34. Yilmaz F., Bereli N., Yavuz H., Denizli A. Supermacroporous hydrophobic affinity cryogels for protein chromatography. Biochem. Eng. J. 2009, 43:272-279.
35. Ye J.L., Yun J.X., Lin D.Q., Xu L.H., Kirsebom H., Shen S.C., Yang G.S., Yao K.J., Guan Y.X., Yao S.J. Poly(hydroxyethyl methacrylate)-based composite cryogel with embedded macroporous cellulose beads for separation of human serum immunoglobulin and albumin. J. Sep. Sci. 2013, 36:3813-3820.
36. Yun J.X., Shen S.C., Chen F., Yao K.J. One-step isolation of adenosine triphosphate from crude fermentation broth of Saccharomyces cerevisiae by anion-exchange chromatography using supermacroporous cryogel. J. Chromatogr. B 2007, 860:57-62.
37. Dong S.S., Chen L., Dai B., Johnson W., Ye J.L., Shen S.C., Yun J.X., Yao K.J., Lin D.Q., Yao S.J. Isolation of immunoglobulin G from bovine milk whey by poly(hydroxyethyl methacrylate)-based anion-exchange cryogel. J. Sep. Sci. 2013, 36:2387-2393.
38. Bhat S., Tripathi A., Kumar A. Supermacroprous chitosan-agarose-gelatin cryogels: in vitro characterization and in vivo assessment for cartilage tissue engineering. J.R. Soc. Interface 2011, 8:540-554.
39. Bloch K., Vanichkin A., Damshkaln L.G., Lozinsky V.I., Vardi P. Vascularization of wide pore agarose-gelatin cryogel scaffolds implanted subcutaneously in diabetic and non-diabetic mice. Acta Biomater. 2010, 6:1200-1205.
40. Dainiak M.B., Allan I.U., Savina I.N., Cornelio L., James E.S., James S.L., Mikhalovsky S.V., Jungvid H., Galaev I.Y. Gelatin-fibrinogen cryogel dermal matrices for wound repair: Preparation, optimisation and in vitro study. Biomaterials 2010, 31:67-76.
41. Jurga M., Dainiak M.B., Sarnowska A., Jablonska A., Tripathi A., Plieva F.M., Savina I.N., Strojek L., Jungvid H., Kumar A., Lukomska B., Domanska-Janik K., Forraz N., McGuckin C.P. The performance of laminin-containing cryogel scaffolds in neural tissue regeneration. Biomaterials 2011, 32:3423-3434.
42. Kathuria N., Tripathi A., Kar K.K., Kumar A. Synthesis and characterization of elastic and macroporous chitosan-gelatin cryogels for tissue engineering. Acta Biomater. 2009, 5:406-418.
43. Kirsebom H., Elowsson L., Berillo D., Cozzi S., Inci I., Piskin E., Galaev I.Y., Mattiasson B. Enzyme-catalyzed crosslinking in a partly frozen state: A new way to produce supermacroporous protein structures. Macromol. Biosci. 2013, 13:67-76.
44. Tripathi A., Kumar A. Multi-featured macroporous agarose-alginate cryogel: synthesis and characterization for bioengineering applications. Macromol. Biosci. 2011, 11:22-35.
45. Börner R.A., Zaushitsyna O., Berillo D., Scaccia N., Mattiasson B., Kirsebom H. Immobilization of Clostridium acetobutylicum DSM 792 as macroporous aggregates through cryogelation for butanol production. Process Biochem. 2014, 49:10-18.
46. Jain E., Karande A.A., Kumar A. Supermacroporous polymer-based cryogel bioreactor for monoclonal antibody production in continuous culture using hybridoma cells. Biotechnol. Prog. 2011, 27:170-180.
47. Jespersen G.R., Matthiesen F., Pedersen A.K., Andersen H.S., Kirsebom H., Nielsen A.L. A thiol functionalized cryogel as a solid phase for selective reduction of a cysteine residue in a recombinant human growth hormone variant. J. Biotechnol. 2014, 173:76-85.
48. Kuyukina M.S., Ivshina I.B., Kamenskikh T.N., Bulicheva M.V., Stukova G.I. Survival of cryogel-immobilized Rhodococcus strains in crude oil-contaminated soil and their impact on biodegradation efficiency. Int. Biodeter. Biodegr. 2013, 84:118-125.
49. Szczesna-Antczak M., Antczak T., Bielecki S. Stability of extracellular proteinase productivity by Bacillus subtilis cells immobilized in PVA-cryogel. Enzyme Microb. Tech. 2004, 34:168-176.
50. Velickova E., Winkelhausen E., Kuzmanova S., Cvetkovska M., Tsvetanov C. Hydroxyethylcellulose cryogels used for entrapment of Saccharomyces cerevisiae cells. React. Funct. Polym. 2009, 69:688-693.
51. Alkan H., Bereli N., Baysal Z., Denizli A. Antibody purification with protein A attached supermacroporous poly(hydroxyethyl methacrylate) cryogel. Biochem. Eng. J. 2009, 45:201-208.
52. Alkan H., Bereli N., Baysal Z., Denizli A. Selective removal of the autoantibodies from rheumatoid arthritis patient plasma using protein A carrying affinity cryogels. Biochem. Eng. J. 2010, 51:153-159.
53. Cramer S.M., Holstein M.A. Downstream bioprocessing: recent advances and future promise. Curr. Opin. Chem. Eng. 2011, 1:27-37.
54. Gagnon P. Technology trends in antibody purification. J. Chromatogr. A 2012, 1221:57-70.
55. Low D., O'Leary R., Pujar N.S. Future of antibody purification. J. Chromatogr. B 2007, 848:48-63.
56. Newcombe C., Newcombe A.R. Antibody production: polyclonal-derived biotherapeutics. J. Chromatogr. B 2007, 848:2-7.
57. Prather K.J., Sagar S., Murphy J., Chartrain M. Industrial scale production of plasmid DNA for vaccine and gene therapy: plasmid design, production, and purification. Enzyme Microb. Technol. 2003, 33:865-883.
58. Shukla A.A., Thömmes J. Recent advances in large-scale production of monoclonal antibodies and related proteins. Trends Biotechnol. 2010, 28:253-261.
59. Perçin I., Sağlar E., Yavuz H., Aksöz E., Denizli A. Poly(hydroxyethyl methacrylate) based affinity cryogel for plasmid DNA purification. Int. J. Biol. Macromol. 2011, 48:577-582.
60. Srivastava A., Shakya A.K., Kumar A. Boronate affinity chromatography of cells and biomacromolecules using cryogel matrices. Enzyme Microb. Technol. 2012, 51:373-381.
61. Bak H., Thomas O.R.T. Evaluation of commercial chromatographic adsorbents for the direct capture of polyclonal rabbit antibodies from clarified antiserum. J. Chromatogr. B 2007, 848:116-130.
62. Bresolin I.T.L., Borsoi-Ribeiro M., Caro J.R., dos Santos F.P., de Castro M.P., Bueno S.M.A. Adsorption of human serum proteins onto TREN-agarose: Purification of human IgG by negative chromatography. J. Chromatogr. B 2009, 877:17-23.
63. Bresolin I.T.L., de Souza M.C.M., Bueno S.M.A. A new process of IgG purification by negative chromatography: adsorption aspects of human serum proteins onto ω-aminodecyl-agarose. J. Chromatogr. B 2010, 878:2087-2093.
64. Chhatre S., Francis R., Titchener-Hooker N.J., Newcombe A.R., Keshavarz-Moore E. Evaluation of a novel agarose-based synthetic ligand adsorbent for the recovery of antibodies from ovine serum. J. Chromatogr. B 2007, 860:209-217.
65. Tong H.F., Lin D.Q., Pan Y., Yao S.J. A new purification process for goose immunoglobulin IgY(δFc) with hydrophobic charge-induction chromatography. Biochem. Eng. J. 2011, 56:205-211.
66. Daoud-Attieh M., Chaib H., Armutcu C., Uzun L., Elkak A., Denizli A. Immunoglobulin G purification from bovine serum with pseudo-specific supermacroporous cryogels. Sep. Purif. Technol. 2013, 118:816-822.
67. Bakhshpour M., Bereli N., Şenel S. Preparation and characterization of thiophilic cryogels with2-mercapto ethanol as the ligand for IgG purification. Colloid Surface B 2014, 113:261-268.
68. Zhao W.W., Liu F.F., Shi Q.H., Sun Y. Octapeptide-based affinity chromatography of human immunoglobulin G: comparisons of three different ligands. J. Chromatogr. A 2014, 1359:100-111.
69. Zhang X., Liu W.H., Chen Y.M., Gong A.J., Chen C.F., Xi F. Self-condensing vinyl polymerization of acrylamide. Polymer. Bull. 1999, 43:29-34.
70. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binging. Anal. Biochem. 1976, 72:248-254.
71. Yun J.X., Jespersen G.R., Kirsebom H., Gustavsson P.-E., Mattiasson B., Galaev I.Y. An improved capillary model for describing the microstructure characteristics, fluid hydrodynamics and breakthrough performance of proteins in cryogel beds. J. Chromatogr. A 2011, 1218:5487-5497.
72. Persson P., Baybak O., Plieva F.M., Galaev I.Y., Mattiasson B., Nilsson B., Axelsson A. Characterization of a continuous supermacroporous monolithic matrix for chromatographic separation of large bioparticles. Biotechnol. Bioeng. 2004, 88:224-236.
73. Yun J.X., Kirsebom H., Galaev I.Y., Mattiasson B. Modeling of protein breakthrough performance in cryogel columns by taking into account the overall axial dispersion. J. Sep. Sci. 2009, 32:2601-2607.
74. Yun J.X., Dafoe J.T., Peterson E., Xu L.H., Yao S.J., Daugulis A.J. Rapid freezing cryo-polymerization and microchannel liquid-flow focusing for cryogel beads: adsorbent preparation and characterization of supermacroporous bead-packed bed. J. Chromatogr. A 2013, 1284:148-154.
75. Gutsche R., Bunke G. Modelling the liquid-phase adsorption in packed beds at low Reynolds numbers: An improved hydrodynamic model. Chem. Eng. Sci. 2008, 63:4203-4217.
76. Seguin D., Montillet A., Bmnjail D., Corniti J. Liquid-solid mass transfer in packed beds of variously shaped particles at low Reynolds numbers: experiments and model. Chem. Eng. J. 1996, 63:1-9.
77. Wang C., Dong X.Y., Jiang Z., Sun Y. Enhanced adsorption capacity of cryogel bed by incorporating polymeric resin particles. J. Chromatogr. A 2013, 1272:20-25.
78. Özdural A.R., Alkan A., Kerkhof P.J.A.M. Modeling chromatographic columns: Non-equilibrium packed-bed adsorption with non-linear adsorption isotherms. J. Chromatogr. A 2004, 1041:77-85.