Macroporous polyacrylamide monolithic gels with immobilized metal affinity ligands: The effect of porous structure and ligand coupling chemistry on protein binding

Journal of Molecular Recognition

Volumn 19, Issue 4, 2006, Pages 305-312

  Plieva, Fatima ^b   Bober, Beata ^c   Dainiak, Maria ^b   Galaev, Igor Yu ^a   Mattiasson, Bo ^a  

^a LUND UNIVERSITY   (Sweden)

^b Ideon Science Park   (Sweden)

^c JAGIELLONIAN UNIVERSITY   (Poland)

Author keywords

Bimodal pore size distribution; Cryogel; IDA; Macroporous gel; Monolith

Indexed keywords

ACRYLIC ACID RESIN; DIGLYCINE; HIS HIS HIS HIS HIS HIS; HIS-HIS-HIS-HIS-HIS-HIS; HISTIDINE; IMINO ACID; LACTATE DEHYDROGENASE; LIGAND; LYSOZYME; METAL; OLIGOPEPTIDE; POLYACRYLAMIDE GELS; POLYMER; WATER;

CHEMISTRY; CONFERENCE PAPER; ISOLATION AND PURIFICATION; METABOLISM; PROTEIN BINDING;

ACRYLIC RESINS; HISTIDINE; IMINO ACIDS; L-LACTATE DEHYDROGENASE; LIGANDS; METALS; MURAMIDASE; OLIGOPEPTIDES; POLYMERS; PROTEIN BINDING; WATER;

EID: 33747312609     PISSN: 09523499     EISSN: 10991352     Source Type: Journal    
DOI: 10.1002/jmr.775     Document Type: Conference Paper

References (19)

1. Arvidsson P, Plieva FM, Lozinsky VI, Galaev IY, Mattiasson B. 2003. Direct chromatographic capture of enzyme from crude homogenate using immobilized metal affinity chromatography on a continuous supermacroporous adsorbent. J. Chromatogr. A 986: 275-290.
2. Arvidsson P, Plieva FM, Savina IN, Lozinsky Vl, Fexby S, Bulow L, Galaev IY, Mattiasson B. 2002. Chromatography of microbial cells using continuous supermacroporous affinity and ion-exchange columns. J. Chromatogr. A 977: 27-38.
3. Chen W-Y., Wu C-F, Liu C-C. 1996. Interactions of imidazole and proteins with immobilized Cu(II) ions: effect of structure, salt concentration and pH in affinity and binding capacity. J. Colloid. Interface. Sci. 180: 135-143.
4. Dainiak MB, Kumar A, Plieva FM, Galaev IY, Mattiasson B. 2004. Integrated isolation of antibody fragments from microbial cell culture fluids using supermacroporous cryogels. J. Chromatogr. A 1045: 93-98.
5. Galaev IY, Dainiak MB, Plieva FM, Hatti-Kaul R, Mattiasson B. 2005. High throuput screening of particulate-containing samples using supermacroporous elastic monoliths in microtiter (multiwell) plate format. J. Chromatogr. A 1065: 169-175.
6. Hanora A, Bernaudat F, Plieva FM, Dainiak MB, Bulow L, Galaev IY, Mattiasson B. 2005. Screening of peptide affinity tags using immobilized metal affinity chromatography in 96-well plate format. J. Chromatogr. A 1087: 38-44.
7. Hermansson GT, Mallia AK, Smith PK. 1992. Immobolized Affinity Ligand Techniques. Academic press, Inc.; Harcout Brace Jovanovich, Publishers, San Diego, p. 18.
8. Josic D, Buchacher A. 2001. Application of monoliths as supports for affinity chromatography and fast enzymatic conversion. J. Biochem. Biophys. Methods 49: 153-174.
9. Josic D, Buchacher A, Jungbauer A. 2001. Monoliths as stationary phases for separation of proteins and polynucleotides and enzymatic conversion. J. Chromatogr. 6752: 191-205.
10. Kumar A, Plieva FM, Galaev IY, Mattiasson B. 2003. Affinity fractionation of lymphocytes using supermacroporous monolithic cryogel. J. Immunol. Methods 283: 185-194.
11. Margel S, Rembaum A. 1980. Synthesis and characterization of poly(glutaraldehyde). A potential reagent for protein immobilization and cell separation. Macromolecules 13: 19-24.
12. Persson P, Baybak O, Plieva F, Galaev IY, Mattiasson B, Nilsson N, Axelsson A. 2004. Characterization of a continuous supermacroporous monolithic matrix for chromatographic separation of large bioparticles. Biotechnol. Bioeng. 88: 224-232.
13. Peters EC, Svec F, Frechet JMJ. 1999. Rigid macroporous polymer monoliths. Adv. Mater. 11: 1169-1181.
14. Plieva FM, Savina IN, Deraz S, Andersson J, Galaev IY, Mattiasson B. 2004. Characterization of supermacroporous monolithic polyacrylamide based matrices designed for chromatography of bioparticles. J. Chromatogr. B 807: 129-137.
15. Plieva FM, Karlsson M, Aguilar M-R, Gomez D, Mikhalovsky S, Galaev IY. 2005. Pore structure in supermacroporous polyacrylamide based cryogels. Soft Matter 1: 303-309.
16. Savina IN, Galaev IY, Mattiasson B. 2005. Graft polymerization of acrylic acid onto macroporous polyacrylamide gel (cryogel) initiated by potassium diperiodatecuprate. Polymer 46: 9596-9603.
17. Sharma S, Agarwal GP. 2001. Interactions of proteins with immobilized metal ions: a comparative analysis using various isotherm models. Analyt. Biochem. 288: 126-140.
18. Svec F, Frechet JMJ. 1999. Molded rigid monolithic porous polymers: an inexpensive, efficient, and versatile alternative to beads for the design of materials for numerous applications. Ind. Eng. Chem. Res. 38: 34-48.
19. Yeom C-K, Lee K-H. 1996. Pervaporation separation of water-acetic acid mixtures through poly(vinyl alcohol) membranes crosslinked with glutaraldehyde. J. Membr. Sci. 109: 257-265.