Isolation of lysozyme from chicken egg white using polyacrylamide-based cation-exchange cryogel

Chinese Journal of Chemical Engineering

Volumn 19, Issue 5, 2011, Pages 876-880

  Yan, Luding ^a   Shen, Shaochuan ^a   Yun, Junxian ^a   Yao, Kejian ^a  

^a ZHEJIANG UNIVERSITY OF TECHNOLOGY   (China)

Author keywords

cation exchange cryogel; chicken egg white; isolation; lysozyme; sequential elution

Indexed keywords

CHICKEN EGG WHITE; CRYOGELS; ISOLATION; LYSOZYME; SEQUENTIAL ELUTION;

ANIMALS; CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; ELECTROPHORESIS; FUNCTIONAL GROUPS; POSITIVE IONS; SODIUM CHLORIDE; SODIUM SULFATE;

ENZYMES;

EID: 81355160862     PISSN: 10049541     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1004-9541(11)60068-2     Document Type: Conference Paper

References (21)

1. F.E. Cunningham, V.A. Proctor, S.J. Goetsch "Egg-white lysozyme as a food preservative: An overview" Worlds. Poultry. Sci. J. 47 2 1991 141 163
2. B. Masschalck, C.W. Michiels "Antimicrobial properties of lysozyme in relation to foodborne vegetative bacteria" Crit. Rev. Microbiol. 29 3 2003 191 214 (Pubitemid 37204782)
3. . Mecitoglu, A. Yemenicioglu, A. Arslanoglu, Z.S. ElmacI, F. Korel, A.E. etin "Incorporation of partially purified hen egg white lysozyme into zein films for antimicrobial food packaging" Food. Res. Int. 39 1 2006 12 21 (Pubitemid 41455489)
4. S. Das, S. Banerjee, J.D. Gupta "Experimental evaluation of preventive and therapeutic potentials of lysozyme" Chemotherapy 38 5 1992 350 357 (Pubitemid 23009983)
5. F. Murakami, T. Sasaki, T. Sugahara "Lysozyme stimulates immunoglobulin production by human-human hybridoma and human peripheral blood lymphocytes" Cytotechnology 24 2 1997 177 182 (Pubitemid 27222180)
6. A. Derazshamshir, B. Ergün, G. Pesint, M. Odabai "Preparation of Zn2+-chelated poly(HEMA-MAH) cryogel for affinity purification of chicken egg lysozyme" J. Appl. Polym. Sci. 109 5 2008 2905 2913
7. D. Aktas Uygun, A.A. Karagözler, S. Akgöl, A. Denizli "Magnetic hydrophobic affinity nanobeads for lysozyme separation" Mater. Sci. Eng. C. 29 7 2009 2165 2173
8. M. Mayani, D.M. Filipe Carlos, R. Ghosh "Cascade ultrafiltration systems-Integrated processes for purification and concentration of lysozyme" J. Membr. Sci. 347 1-2 2010 150 158
9. R. Dembczynski, W. Bialas, K. Regulski, T. Jankowski "Lysozyme extraction from hen egg white in an aqueous two-phase system composed of ethylene oxide-propylene oxide thermoseparating copolymer and potassium phosphate" Process. Biochem. 45 3 2010 369 374
10. K.J. Yao, J.X. Yun, S.C. Shen, F. Chen "In-situ graft-polymerization preparation of cation-exchange supermacroporous cryogel with sulfo groups in glass columns" J. Chromatogr. A. 1157 1-2 2007 246 251 (Pubitemid 46963313)
11. F.M. Plieva, J. Andersson, I.Y. Galaev, B. Mattiasson "Characterization of polyacrylamide based monolithic columns" J. Sep. Sci. 27 10-11 2004 828 836 (Pubitemid 39136469)
12. K.J. Yao, S.C. Shen, J.X. Yun, L.H. Wang, X.J. He, X.M. Yu "Preparation of poly-acrylamide-based supermacroporous monolithic cryogel beds under freezing-temperature variation conditions" Chem. Eng. Sci. 61 20 2006 6701 6708
13. F. Chen, K.J. Yao, S.C. Shen, J.X. Yun "Influence of grafting conditions on the properties of polyacrylamide-based cation-exchange cryogels grafted with 2-acrylamido-2-methyl-1-propane-sulfonic acid" Chem. Eng. Sci. 63 1 2008 71 77 (Pubitemid 350101470)
14. 2 nanoparticles" Chin. J. Chem. Eng. 18 4 2010 667 671
15. C. Guerin-Dubiard, M. Pasco, A. Hietanen, A.Q. del Bosque, F. Nau, T. Croguennec "Hen egg white fractionation by ion-exchange chromatography" J. Chromatogr. A. 1090 1-2 2005 58 67 (Pubitemid 41207664)
16. U.K. Laemmli "Cleavage of structural proteins during the assembly of the head of bacteriophage T4" Nature 227 5259 1970 680 685
17. M.M. Bradford "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding" Anal. Biochem. 72 1-2 1976 248 254
18. K.J. Yao, J.X. Yun, S.C. Shen, L.H. Wang, X.J. He, X.M. Yu "Characterization of a novel continuous supermacroporous monolithic cryogel embedded with nanoparticles for protein chromatography" J. Chromatogr. A. 1109 1 2006 103 110
19. X.J. He, K.J. Yao, S.C. Shen, J.X. Yun "Freezing characteristics of acrylamide-based aqueous solution used for the preparation of supermacroporous cryogels via cryo-copolymerization" Chem. Eng. Sci. 62 5 2007 1334 1342 (Pubitemid 46109425)
20. L. Stevens "Egg-White Proteins" Comp. Biochem. Phys. B. 100 1 1991 1 9
21. R. Ghosh, Z.F. Gui "Purification of lysozyme using ultrafiltration" Biotechnol. Bioeng. 68 2 2000 191 203