Droplet-based microfluidics for high-throughput screening of a metagenomic library for isolation of microbial enzymes

Biosensors and Bioelectronics

Volumn 67, Issue , 2015, Pages 379-385

  Hosokawa, Masahito ^a,b   Hoshino, Yuri ^a   Nishikawa, Yohei ^a   Hirose, Tomotada ^a   Yoon, Dong Hyun ^a   Mori, Tetsushi ^a,b   Sekiguchi, Tetsushi ^a   Shoji, Shuichi ^a   Takeyama, Haruko ^a,b  

^a WASEDA UNIVERSITY   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

Droplet; Lipolytic enzyme; Metagenomic library; Microfluidics; Single cell analysis

Indexed keywords

CLONING; DROPS; MICROFLUIDICS; SUBSTRATES; THROUGHPUT;

DROPLET-BASED MICROFLUIDICS; HIGH THROUGHPUT SCREENING; LIPOLYTIC ENZYMES; METAGENOMIC LIBRARIES; MICROBIAL ENZYMES; PUTATIVE SIGNAL PEPTIDE; SCREENING APPROACHES; SINGLECELL ANALYSIS;

ENZYMES;

ESTERASE; MICROBIAL ENZYME; OIL; PROTEIN ESTT1; UNCLASSIFIED DRUG; TRIACYLGLYCEROL LIPASE;

ARTICLE; BACTERIAL CELL; CATALYSIS; CELL ACTIVITY; CELL ENCAPSULATION; CELL GROWTH; CONTROLLED STUDY; CYTOPLASM; ENCAPSULATION; ENZYME ISOLATION; HIGH THROUGHPUT SCREENING; LIPOLYSIS; METAGENOMICS; MICROFLUIDICS; MOLECULAR CLONING; NONHUMAN; SOIL ANALYSIS; ENZYME SPECIFICITY; GENETIC PROCEDURES; GENETICS; ISOLATION AND PURIFICATION; MICROBIOLOGY;

QUERCUS;

BIOSENSING TECHNIQUES; HIGH-THROUGHPUT SCREENING ASSAYS; LIPASE; METAGENOMICS; MICROFLUIDICS; SOIL MICROBIOLOGY; SUBSTRATE SPECIFICITY;

EID: 84922247524     PISSN: 09565663     EISSN: 18734235     Source Type: Journal    
DOI: 10.1016/j.bios.2014.08.059     Document Type: Article

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