Detecting a quasi-stable imine species on the reaction pathway of SHV-1 β-lactamase and 6β-(hydroxymethyl)penicillanic acid sulfone

Biochemistry

Volumn 54, Issue 3, 2015, Pages 734-743

  Che, Tao ^a   Rodkey, Elizabeth A ^a   Bethel, Christopher R ^b   Shanmugam, Sivaprakash ^a   Ding, Zhe ^a   Pusztai Carey, Marianne ^a   Nottingham, Michael ^c   Chai, Weirui ^c   Buynak, John D ^c   Bonomo, Robert A ^a,b   Van Den Akker, Focco ^a   Carey, Paul R ^a  

^a CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b LOUIS STOKES CLEVELAND VA MEDICAL CENTER   (United States)

^c SOUTHERN METHODIST UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARBOXYLIC ACIDS; ENZYMES; HYDROLYSIS; IONS; MOLECULES; NITROGEN COMPOUNDS; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; REACTION KINETICS; SINGLE CRYSTALS; X RAY CRYSTALLOGRAPHY;

AMINOACRYLATES; CHEMICAL EQUATIONS; CRYSTALLOGRAPHIC DATA; HYDROXYMETHYL GROUPS; MECHANISTIC PROPERTIES; RAMAN STUDIES; REACTION PATHWAYS; WATER MOLECULE;

METHANOL;

BETA LACTAMASE; CLAVULANIC ACID; IMINE; SULBACTAM; TAZOBACTAM; 6BETA-(HYDROXYMETHYL)PENICILLANIC ACID SULFONE; BETA LACTAMASE INHIBITOR; BETA-LACTAMASE PIT-2; SOLUTION AND SOLUBILITY;

ARTICLE; BACTERIAL CELL; CATALYSIS; CHEMICAL STRUCTURE; CONTROLLED STUDY; CRYSTAL STRUCTURE; CRYSTALLIZATION; DEACYLATION; ENZYME INACTIVATION; ENZYME MECHANISM; HYDROGEN BOND; HYDROLYSIS; IN VITRO STUDY; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PH; PRIORITY JOURNAL; PROTON NUCLEAR MAGNETIC RESONANCE; RAMAN SPECTROMETRY; REACTION ANALYSIS; REACTION TIME; STEADY STATE; SUBSTITUTION REACTION; X RAY ANALYSIS; X RAY CRYSTALLOGRAPHY; ANALOGS AND DERIVATIVES; BIOCATALYSIS; CHEMISTRY; DRUG EFFECTS; ENZYME ACTIVE SITE; ESCHERICHIA COLI; KINETICS; METABOLISM; MICROBIAL SENSITIVITY TEST; NORMAL DISTRIBUTION; SOLUTION AND SOLUBILITY;

BETA-LACTAMASE INHIBITORS; BETA-LACTAMASES; BIOCATALYSIS; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; HYDROLYSIS; IMINES; KINETICS; MICROBIAL SENSITIVITY TESTS; NORMAL DISTRIBUTION; SOLUTIONS; SPECTRUM ANALYSIS, RAMAN; SULBACTAM;

EID: 84922065641     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi501197t     Document Type: Article

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