Identification of therapeutic small-molecule leads in cultured cells using multiplexed pathway reporter readouts

Methods in Molecular Biology

Volumn 1263, Issue , 2015, Pages 3-16

  Kulak, Ozlem ^a   Yamaguchi, Kiyoshi ^a   Lum, Lawrence ^a  

^a NONE

Author keywords

Dot blotting; Kras; Luciferase assay; RNAi; Small molecule screening; TP53; Wnt

Indexed keywords

K RAS PROTEIN; PROTEIN P53; SONIC HEDGEHOG PROTEIN; WNT PROTEIN; LUCIFERASE; MOLECULAR LIBRARY;

ARTICLE; BLOTTING; CELL CULTURE; CELL LYSATE; CONTROLLED STUDY; DOT BLOTTING; HCT116 CELL LINE; HIGH THROUGHPUT SCREENING; LUCIFERASE ASSAY; MOLECULAR LIBRARY; PRIORITY JOURNAL; REPORTER GENE; SIGNAL TRANSDUCTION; TRANSIENT TRANSFECTION; WESTERN BLOTTING; WNT SIGNALING PATHWAY; CELL LINE; DRUG EFFECTS; DRUG SCREENING; GENE EXPRESSION; GENETIC TRANSFECTION; GENETICS; HUMAN; LUMINESCENCE; METABOLISM; PROCEDURES;

CELL LINE; CELLS, CULTURED; DRUG EVALUATION, PRECLINICAL; GENE EXPRESSION; GENES, REPORTER; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; LUCIFERASES; LUMINESCENT MEASUREMENTS; SIGNAL TRANSDUCTION; SMALL MOLECULE LIBRARIES; TRANSFECTION;

EID: 84922032208     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-2269-7_1     Document Type: Article

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