Drug affinity responsive target stability (DARTS) for small-molecule target identification

Methods in Molecular Biology

Volumn 1263, Issue , 2015, Pages 287-298

  Pai, Melody Y ^a   Lomenick, Brett ^a   Hwang, Heejun ^a   Schiestl, Robert ^a   McBride, William ^a   Loo, Joseph A ^a   Huang, Jing ^a  

^a NONE

Author keywords

Drugs; Immunoblotting; Mass spectrometry; Metabolites; Natural products; Proteomics; Small molecules; Target identification

Indexed keywords

PROTEIN HYDROLYSATE; LIGAND; MOLECULAR LIBRARY; PROTEIN; PROTEIN BINDING;

ARTICLE; DRUG AFFINITY RESPONSIVE TARGET STABILITY; HEK293 CELL LINE; IMMUNOBLOTTING; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN STABILITY; PROTEIN TARGETING; THERMOSTABILITY; WESTERN BLOTTING; CHEMISTRY; METABOLISM; MOLECULAR LIBRARY; PROCEDURES; PROTEOMICS; REPRODUCIBILITY;

LIGANDS; PROTEIN BINDING; PROTEINS; PROTEOMICS; REPRODUCIBILITY OF RESULTS; SMALL MOLECULE LIBRARIES;

EID: 84922021953     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-2269-7_22     Document Type: Article

References (25)

1. O’Connor CJ, Laraia L, Spring DR (2011) Chemical genetics. Chem Soc Rev 40: 4332-4345
2. Yang GX, Li X, Snyder M (2012) Investigating metabolite-protein interactions: an overview of available techniques. Methods 57: 459-466
3. McFedries A, Schwaid A, Saghatelian A (2013) Methods for the elucidation of protein-small molecule interactions. Chem Biol 20: 667-673
4. Rask-Andersen M, Masuram S, Schioth HB (2014) The druggable genome: evaluation of drug targets in clinical trials suggests major shifts in molecular class and indication. Annu Rev Pharmacol Toxicol 54: 9-26
5. Lomenick B, Olsen RW, Huang J (2011) Identification of direct protein targets of small molecules. ACS Chem Biol 6: 34-46
6. Ong SE et al (2012) Identifying cellular targets of small-molecule probes and drugs with biochemical enrichment and SILAC. Methods Mol Biol 803: 129-140
7. Ziegler S et al (2013) Target identification for small bioactive molecules: finding the needle in the haystack. Angew Chem Int Ed Engl 52: 2744-2792
8. Futamura Y, Muroi M, Osada H (2013) Target identification of small molecules based on chemical biology approaches. Mol Biosyst 9: 897-914
9. Lomenick B et al (2009) Target identification using drug affinity responsive target stability (DARTS). Proc Natl Acad Sci U S A 106: 21984-21989
10. Robinson TJ et al (2013) High-throughput screen identifies disulfiram as a potential therapeutic for triple-negative breast cancer cells: interaction with IQ motif-containing factors. Cell Cycle 12: 3013-3024
11. Chin RM et al (2014) The metabolite alphaketoglutarate extends lifespan by inhibiting ATP synthase and TOR. Nature. doi: 10.1038/ nature13264
12. Lomenick B et al (2011) Target identification using drug affinity responsive target stability (DARTS). Curr Protoc Chem Biol 3: 163-180
13. Tohda C et al (2012) Diosgenin is an exogenous activator of 1, 25D (3) -MARRS/Pdia3/ ERp57 and improves Alzheimer’s disease pathologies in 5XFAD mice. Sci Rep 2: 535
14. Sun W et al (2014) Chemical signatures and new drug targets for gametocytocidal drug development. Sci Rep 4: 3743
15. Aghajan M et al (2010) Chemical genetics screen for enhancers of rapamycin identifies a specific inhibitor of an SCF family E3 ubiquitin ligase. Nat Biotechnol 28: 738-742
16. Chen T et al (2011) Chemical genetics identify eIF2alpha kinase heme-regulated inhibitor as an anticancer target. Nat Chem Biol 7: 610-616
17. Gao S et al (2012) The chemistry and biology of nakiterpiosin-C-nor-D-homosteroids. Synlett 16: 2298-2310
18. Xu S et al (2013) Stabilization of MDA-7/ IL-24 for colon cancer therapy. Cancer Lett 335: 421-430
19. Lim M et al (2014) Ligand-independent and tissue-selective androgen receptor inhibition by pyrvinium. ACS Chem Biol 9: 692-702
20. Li H et al (2014) Drug design targeting protein-protein interactions (PPIs) using multiple ligand simultaneous docking (MLSD) and drug repositioning: discovery of raloxifene and bazedoxifene as novel inhibitors of IL-6/ GP130 interface. J Med Chem 57: 632-641
21. Molina DM et al (2013) Monitoring drug target engagement in cells and tissues using the cellular thermal shift assay. Science 341: 84-87
22. Kragten E et al (1998) Glyceraldehyde-3-phosphate dehydrogenase, the putative target of the antiapoptotic compounds CGP 3466 and R- (-) -deprenyl. J Biol Chem 273: 5821-5828
23. Lundberg E et al (2010) Defining the transcriptome and proteome in three functionally different human cell lines. Mol Syst Biol 6: 450
24. Geiger T et al (2012) Comparative proteomic analysis of eleven common cell lines reveals ubiquitous but varying expression of most proteins. Mol Cell Proteomics 11: M111014050
25. Geiger T et al (2013) Initial quantitative proteomic map of 28 mouse tissues using the SILAC mouse. Mol Cell Proteomics 12: 1709-1722