In vivo screening for substrates of protein kinase a using a combination of proteomic approaches and pharmacological modulation of kinase activity

Cell Structure and Function

Volumn 40, Issue 1, 2015, Pages 1-12

  Hamaguchi, Tomonari ^a   Nakamuta, Shinichi ^a   Funahashi, Yasuhiro ^a   Takano, Tetsuya ^a   Nishioka, Tomoki ^a   Shohag, Md Hasanuzzaman ^a   Yura, Yoshimitsu ^a   Kaibuchi, Kozo ^a   Amano, Mutsuki ^a  

^a NAGOYA UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

Author keywords

Mass spectrometry; Phosphorylation; Protein kinase A; Rho kinase

Indexed keywords

CYCLIC AMP DEPENDENT PROTEIN KINASE; FORSKOLIN; ISOBUTYLMETHYLXANTHINE; N [2 (4 BROMOCINNAMYLAMINO)ETHYL] 5 ISOQUINOLINESULFONAMIDE; PHOSPHOPROTEIN; PROTEIN ROCK2; RHO KINASE; SERINE; UNCLASSIFIED DRUG; ISOQUINOLINE DERIVATIVE; N-(2-(4-BROMOCINNAMYLAMINO)ETHYL)-5-ISOQUINOLINESULFONAMIDE; PROTEIN BINDING; SULFONAMIDE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; EMBRYO; ENZYME INHIBITION; ENZYME PHOSPHORYLATION; ENZYME SUBSTRATE; HELA CELL LINE; IMMUNOPRECIPITATION; IN VITRO STUDY; IN VIVO STUDY; LIQUID CHROMATOGRAPHY; MALE; MOUSE; NONHUMAN; PROTEIN ANALYSIS; PROTEOMICS; SCREENING; SIGNAL TRANSDUCTION; TANDEM MASS SPECTROMETRY; AMINO ACID SEQUENCE; ANIMAL; CHEMISTRY; CHLOROCEBUS AETHIOPS; COS 1 CELL LINE; DRUG EFFECTS; HUMAN; MASS SPECTROMETRY; METABOLISM; PHOSPHORYLATION;

1-METHYL-3-ISOBUTYLXANTHINE; AMINO ACID SEQUENCE; ANIMALS; CERCOPITHECUS AETHIOPS; COLFORSIN; COS CELLS; CYCLIC AMP-DEPENDENT PROTEIN KINASES; HELA CELLS; HUMANS; IMMUNOPRECIPITATION; ISOQUINOLINES; MASS SPECTROMETRY; PHOSPHORYLATION; PROTEIN BINDING; PROTEOMICS; RHO-ASSOCIATED KINASES; SULFONAMIDES;

EID: 84921954827     PISSN: 03867196     EISSN: 13473700     Source Type: Journal    
DOI: 10.1247/csf.14014     Document Type: Article

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