Catalytic efficiency of chitinase-D on insoluble chitinous substrates was improved by fusing auxiliary domains

PLoS ONE

Volumn 10, Issue 1, 2015, Pages

  Madhuprakash, Jogi ^a   El Gueddari, Nour Eddine ^b   Moerschbacher, Bruno M ^b   Podile, Appa Rao ^a  

^a UNIVERSITY OF HYDERABAD   (India)

^b UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA CHITIN; BETA CHITIN; BINDING PROTEIN; CARBOHYDRATE DERIVATIVE; CHITIN BINDING PROTEIN 21; CHITIN BINDING PROTEIN 21 CHITINASE D FUSION CHIMERA; CHITIN BINDING PROTEIN 21 CHITINASE D POLYCYSTIC KIDNEY DISEASE DOMAIN FUSION CHIMERA; CHITIN DERIVATIVE; CHITINASE; CHITINASE D; CHITINASE D CHITIN BINDING PROTEIN 21 FUSION CHIMERA; CHITINASE D POLYCYSTIC KIDNEY DISEASE DOMAIN FUSION CHIMERA; CHITOTETRAOSE; COLLOIDAL CHITIN; HYBRID PROTEIN; POLYCYSTIC KIDNEY DISEASE DOMAIN CHITINASE D CHITIN BINDING PROTEIN 21 FUSION CHIMERA; POLYCYSTIC KIDNEY DISEASE DOMAIN CHITINASE D FUSION CHIMERA; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; CHITIN;

AMINO TERMINAL SEQUENCE; ARTICLE; AUXILIARY DOMAIN; BINDING AFFINITY; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CATALYTIC EFFICIENCY; COMPARATIVE STUDY; CONTROLLED STUDY; DOMAIN FUSION; ENZYMATIC DEGRADATION; ENZYME ACTIVITY; ENZYME ISOLATION; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE COMPLEX; GLYCOSYLATION; INCUBATION TIME; LIGAND BINDING; MOLECULAR BIOLOGY; POLYCYSTIC KIDNEY DISEASE DOMAIN; PROTEIN DOMAIN; PROTEIN EXPRESSION; SERRATIA; SERRATIA PROTEAMACULANS; STEADY STATE; STRUCTURE ACTIVITY RELATION; ZYMOGRAPHY; BIOCATALYSIS; CHEMISTRY; ENZYMOLOGY; GENETICS; METABOLISM; PROTEIN TERTIARY STRUCTURE;

SERRATIA PROTEAMACULANS;

BACTERIAL PROTEINS; BIOCATALYSIS; CHITIN; CHITINASES; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; SERRATIA; SUBSTRATE SPECIFICITY;

EID: 84921933035     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0116823     Document Type: Article

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