Chitin binding proteins act synergistically with chitinases in Serratia proteamaculans 568

PLoS ONE

Volumn 7, Issue 5, 2012, Pages

  Purushotham, Pallinti ^a   Arun, P V Parvati Sai ^a   Prakash, Jogadhenu S S ^a   Podile, Appa Rao ^a  

^a UNIVERSITY OF HYDERABAD   (India)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING PROTEIN; CHITIN; CHITIN BINDING PROTEIN; CHITINASE; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; CARRIER PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; BINDING AFFINITY; GENE; GENE OVEREXPRESSION; GENE SEQUENCE; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN BINDING; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN FUNCTION; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY; SERRATIA; SERRATIA PROTEAMACULANS 568; SP CBP GENE; BACTERIAL GENOME; CHEMISTRY; GENETICS; METABOLISM; PHYSIOLOGY;

SERRATIA PROTEAMACULANS;

BACTERIAL PROTEINS; CARRIER PROTEINS; CHITINASE; GENOME, BACTERIAL; RECOMBINANT PROTEINS; SERRATIA;

EID: 84860733560     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0036714     Document Type: Article

References (29)

1. Rinaudo M, (2006) Chitin and chitosan: Properties and applications. Prog Polym Sci 31: 603-632.
2. Neeraja Ch, Anil K, Purushotham P, Suma K, Sarma PVSRN, et al. (2010c) Biotechnological approaches to develop bacterial chitinases as a bioshield against fungal diseases of plants. Crit Rev Biotechnol 30: 231-241.
3. Zamil SS, Ahmad S, Choi MH, Yoon SC, (2010) Production of poly-N-acetylglucosamine by Staphylococcus saprophyticus BMSZ711: Characterization and production optimization. Bioresour Technol 101: 7177-7180.
4. Vaaje-Kolstad G, Bunæs AC, Mathiesen G, Eijsink VGH, (2009) The chitinolytic system of Lactococcus lactis ssp. Lactis comprises a nonprocessive chitinase and a chitin-binding protein that promotes the degradation of α- and β-chitin. FEBS J 276: 2402-2415.
5. Orikoshi H, Nakayama S, Miyamoto K, Hanato C, Yasuda M, et al. (2005) Roles of four chitinases (ChiA, ChiB, ChiC, and ChiD) in the chitin degradation system of marine bacterium Alteromonas sp. Strain O-7. Appl Environ Microbiol 71: 1811-1815.
6. Eijsink VGH, Vaaje-Kolstad G, Vårum KM, Horn SJ, (2008) Towards new enzymes for biofuels: lessons from chitinase research. Trends Biotechnol 26: 228-235.
7. Vaaje-Kolstad G, Houston DR, Riemen AH, Eijsink VGH, van Aalten DM, (2005a) Crystal structure and binding properties of the Serratia marcescens chitin binding protein CBP21. J Biol Chem 280: 11313-11319.
8. Vaaje-Kolstad G, Horn SJ, van Aalten DM, Synstad B, Eijsink VG, (2005b) The non-catalytic chitin-binding protein CBP21 from Serratia marcescens is essential for chitin degradation. J Biol Chem 280: 28492-28497.
9. van Aalten DMF, Synstad B, Brurberg MB, Hough E, Riise BW, et al. (2000) Structure of a two-domain chitotriosidase from Serratia marcescens at 1.9-Å resolution. Proc Natl Acad Sci U S A 97: 5842-5847.
10. Vaaje-Kolstad G, Westereng B, Horn SJ, Liu Z, Zhai H, et al. (2010) An oxidative enzyme boosting the enzymatic conversion of recalcitrant polysaccharides. Science 330: 219-222.
11. Neeraja C, Subramanyam R, Moerschbacher B, Podile AR, (2010b) Swapping the chitin-binding domain in Bacillus chitinases improves the substrate binding affinity and conformational stability. Mol BioSyst 6: 1492-1502.
12. Taghavi S, Garafola C, Monchy S, Newman L, Hoffman A, et al. (2009) Genome survey and characterization of endophytic bacteria exhibiting a beneficial effect on growth and development of poplar trees. Appl Environ Microbiol 75: 748-757.
13. Henrissat B, Davies G, (1997) Structural and sequence-based classification of glycoside hydrolases. Curr Opin Struct Biol 7: 637-644.
14. Suetake T, Tsuda S, Kawabata S, Miura K, Iwanaga S, et al. (2000) Chitin-binding proteins in invertebrates and plants comprise a common chitin-binding structural motif. J Biol Chem 275: 17929-17932.
15. Suzuki K, Suzuki M, Taiyoji M, Nikaidou N, Watanabe T, (1998) Chitin binding protein (CBP21) in the culture supernatant of Serratia marcescens 2170. Biosci Biotechnol Biochem 62: 128-135.
16. Zeltins A, Schrempf H, (1997) Specific interaction of the Streptomyces chitin-binding protein CHB1 with alpha-chitin - the role of individual tryptophan residues. Eur J Biochem 246: 557-564.
17. Chu HH, Hoang V, Hofemeister J, Schrempf H, (2001) A Bacillus amyloliquefaciens ChbB protein binds beta- and alpha-chitin and has homologues in related strains. Microbiology 147: 1793-1803.
18. Mehmood MA, Xiao X, Hafeez FY, Gai Y, Wang F, (2011) Molecular characterization of the modular chitin binding protein Cbp50 from Bacillus thuringiensis serovar konkukian. Antonie Van Leeuwenhoek 53: 125-136.
19. Vaaje-Kolstad G, Bøhle LA, Gåseidnes S, Dalhus B, Bjørås M, et al. (2012) Characterization of the chitinolytic machinery of Enterococcus faecalis V583 and high-resolution structure of its oxidative CBM33 enzyme. J Mol Biol 416: 239-254.
20. Purushotham P, Sarma PVSRN, Podile AR, (2012) Multiple chitinases of an endophytic Serratia proteamaculans 568 generate chitin oligomers. Bioresour Technol 112: 261-269.
21. Berger LR, Reynolds DM, (1988) Colloidal chitin preparation. Methods Enzymol 161: 140-142.
22. Neeraja C, Moerschbacher B, Podile AR, (2010a) Fusion of cellulose bindin domain to the catalytic domain improves the activity and conformational stability of chitinase in Bacillus licheniformis DSM13. Bioresour Technol 101: 3635-3641.
23. Pace CN, Vajdos F, Fee L, Grimsley G, Gray T, (1995) How to measure and predict the molar absorption coefficient of a protein. Protein Sci 4: 2411-2423.
24. Hardt M, Laine RA, (2004) Mutation of active site residues in the chitin-binding domain ChBDChiA1 from chitinase A1 of Bacillus circulans alters substrate specificity: use of a green fluorescent protein binding assay. Arch Biochem Biophys 426: 286-297.
25. Schnellmann J, Zeltins A, Blaak H, Schrempf H, (1994) The novel lectin-like protein CHB1 is encoded by a chitin-inducible Streptomyces olivaceoviridis gene and binds specifically to crystalline alpha-chitin of fungi and other organisms. Mol Microbiol 13: 807-819.
26. Kolbe S, Fischer S, Becirevic A, Hinz P, Schrempf H, (1998) The Streptomyces reticuli alpha-chitin-binding protein CHB2 and its gene. Microbiology 144: 1291-1297.
27. Saito A, Miyashita K, Biukovic G, Schrempf H, (2001) Characteristics of a Streptomyces coelicolor A3(2) extracellular protein targeting chitin and chitosan. Appl Environ Microbiol 67: 1268-1273.
28. Folders J, Tommassen J, van Loon LC, Bitter W, (2000) Identification of a chitin-binding protein secreted by Pseudomonas aeruginosa. J Bacteriol 182: 1257-1263.
29. Moser F, Irwin D, Chen S, Wilson DB, (2008) Regulation and characterization of Thermobifida fusca carbohydrate-binding module proteins E7 and E8. Biotechnol Bioeng 100: 1066-1077.