Isolation of Giant Plasma Membrane vesicles for evaluation of plasma membrane structure and protein partitioning

Methods in Molecular Biology

Volumn 1232, Issue , 2015, Pages 65-77

  Levental, K R ^a   Levental, I ^a  

^a NONE

Author keywords

GPMV; Lipid rafts; Phase separation; Plasma membrane

Indexed keywords

PROTEIN;

ARTICLE; CELL COMPOSITION; CELL ISOLATION; CELL MEMBRANE; FLUORESCENCE MICROSCOPY; GIANT PLASMA MEMBRANE VESICLE; MAMMAL CELL; MEMBRANE STRUCTURE; MISCIBILITY; PHASE PARTITIONING; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; TEMPERATURE; ANIMAL; CELL CULTURE; CELL FRACTIONATION; CELL ORGANELLE; CHEMISTRY; DEVICES; EQUIPMENT DESIGN; ISOLATION AND PURIFICATION; MAMMAL; MOLECULAR BIOLOGY; PROCEDURES;

EUKARYOTA; MAMMALIA;

ANIMALS; CELL FRACTIONATION; CELL MEMBRANE; CELLS, CULTURED; EQUIPMENT DESIGN; MAMMALS; MOLECULAR BIOLOGY; ORGANELLES; PROTEINS;

EID: 84921822721     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-1752-5_6     Document Type: Article

References (24)

1. Simons K, Ikonen E (1997) Functional rafts in cell membranes. Nature 387(6633):569–572
2. Simons K, Vaz WL (2004) Model systems, lipid rafts, and cell membranes. Annu Rev Biophys Biomol Struct 33:269–295
3. McConnell HM, Vrljic M (2003) Liquid-liquid immiscibility in membranes. Annu Rev Biophys Biomol Struct 32:469–492
4. Kenworthy AK (2008) Have we become overly reliant on lipid rafts? Talking Point on the involvement of lipid rafts in T-cell activation. EMBO Rep 9(6):531–535
5. Munro S (2003) Lipid rafts: elusive or illusive? Cell 115(4):377–388
6. Baumgart T et al (2007) Large-scale fluid/ fluid phase separation of proteins and lipids in giant plasma membrane vesicles. Proc Natl Acad Sci U S A 104(9):3165–3170
7. Levental I et al (2009) Cholesterol-dependent phase separation in cell-derived giant plasma-membrane vesicles. Biochem J 424(2): 163–167
8. Lingwood D, Ries J, Schwille P, Simons K (2008) Plasma membranes are poised for activation of raft phase coalescence at physiological temperature. Proc Natl Acad Sci U S A 105(29):10005–10010
9. Ayuyan AG, Cohen FS (2008) Raft composition at physiological temperature and pH in the absence of detergents. Biophys J 94:2654–2666
10. Sengupta P, Holowka D, Baird B (2007) Fluorescence resonance energy transfer betweenlipid probes detects nanoscopic heterogeneity in the plasma membrane of live cells. Biophys J 92(10):3564–3574
11. Swamy MJ et al (2006) Coexisting domains in the plasma membranes of live cells characterized by spin-label ESR spectroscopy. Biophys J 90(12):4452
12. Eggeling C et al (2009) Direct observation of the nanoscale dynamics of membrane lipids in a living cell. Nature 457(7233):1159–1162
13. Prior IA, Muncke C, Parton RG, Hancock JF (2003) Direct visualization of Ras proteins in spatially distinct cell surface microdomains. J Cell Biol 160(2):165–170
14. Klemm RW et al (2009) Segregation of sphingolipids and sterols during formation of secretory vesicles at the trans-Golgi network. J Cell Biol 185(4):601–612
15. Gerl MJ et al (2012) Quantitative analysis of the lipidomes of the influenza virus envelope and MDCK cell apical membrane. J Cell Biol 196(2):213–221
16. Hancock JF (2006) Lipid rafts: contentious only from simplistic standpoints. Nat Rev Mol Cell Biol 7(6):456–462
17. Sezgin E et al (2012) Elucidating membrane structure and protein behavior using giant plasma membrane vesicles. Nat Protoc 7(6):1042–1051
18. Levental I, Grzybek M, Simons K (2011) Raft domains of variable properties and compositions in plasma membrane vesicles. Proc Natl Acad Sci U S A 108(28):11411–11416
19. Kaiser HJ et al (2009) Order of lipid phases in model and plasma membranes. Proc Natl Acad Sci U S A 106(39):16645–16650
20. Levental I, Lingwood D, Grzybek M, Coskun U, Simons K (2010) Palmitoylation regulates raft affinity for the majority of integral raft proteins. Proc Natl Acad Sci U S A 107(51): 22050–22054
21. Sengupta P, Hammond A, Holowka D, Baird B (2008) Structural determinants for partitioning of lipids and proteins between coexisting fluid phases in giant plasma membrane vesicles. Biochim Biophys Acta 1778(1):20–32
22. Diaz-Rohrer BB, Levental KR, Simons K, Levental I (2014) Membrane raft association is a determinant of plasma membrane localization. Proc Natl Acad Sci 111(23):8500–8505
23. Scott RE (1976) Plasma membrane vesiculation: a new technique for isolation of plasma membranes. Science 194(4266):743–745
24. Zhou Y et al (2013) Bile acids modulate signaling by functional perturbation of plasma membrane domains. J Biol Chem. 288(50): 35660–35670