Short hairpin rnas of designed sequences can be extracellularly produced by the marine bacterium rhodovulum sulfidophilum

Journal of General and Applied Microbiology

Volumn 60, Issue 6, 2015, Pages 222-226

  Nagao, Nobuyoshi ^a   Suzuki, Hiromichi ^a   Numano, Rika ^a   Umekage, So ^a   Kikuchi, Yo ^a  

^a TOYOHASHI UNIVERSITY OF TECHNOLOGY   (Japan)

Author keywords

Extracellular RNA; Marine Bacteria; Rhodovulum sulfidophilum; RNA medicine; Short hairpin RNA

Indexed keywords

APTAMER; DOUBLE STRANDED RNA; SHORT HAIRPIN RNA; STREPTAVIDIN;

ARTICLE; CONTROLLED STUDY; EXTRACELLULAR SPACE; NONHUMAN; RHODOVULUM; RHODOVULUM SULFIDOPHILUM; RNA PROCESSING; RNA SEQUENCE; RNA SYNTHESIS; RNA TRANSCRIPTION;

BACTERIA (MICROORGANISMS); RHODOVULUM SULFIDOPHILUM;

EID: 84921772046     PISSN: 00221260     EISSN: 13498037     Source Type: Journal    
DOI: 10.2323/jgam.60.222     Document Type: Article

References (16)

1. Ando, T, Tanaka, T., and Kikuchi, Y. (2003) Substrate specificity of E. coli RNase P ribozyme is dependent on the concentration of magnesium ion. J. Biochem., 133, 445-451.
2. Castanotto, D. and Rossi, J. J (2009) The promises and pitfalls of RNA-interference-based therapeutics. Nature, 457, 426-433.
3. Chomczynski, P. and Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem., 162, 156-159.
4. Kikuchi, Y. (2010) Extracellular nucleic acids of the marine phototrophic bacterium Rhodovulum sulfidophilum and related bacteria: Physiology and biotechnology. In Extracellular Nucleic Acids, ed. by Kikuchi, Y. and Rykova, E. Y., Springer-Verlag, Heidelberg, pp. 55-67.
5. Kikuchi, Y., Suzuki, H., and Umekage, S. (2010) Produktion definierter RNAs im Kulturueberstand von Bakterien. LABORWELT, 11, 6-7.
6. Kyzer, S., Ha, K. S., Landick, R., and Palangat, M. (2007) Direct versus limited-step reconstitution reveals key features of an RNA hairpin-stabilized paused transcription complex. J. Biol. Chem., 282, 19020-19028.
7. Nagashima, K. V. P., Hiraishi, A., Shimada, K., and Matsuura, K. (1997) Horizontal transfer of genes coding for the photosynthetic reaction centers of purple bacteria. J. Mol. Evol., 45, 131-136.
8. Nellen, W. and Hammann, C. (2005) Small RNAs: Analysis and regulatory functions. Nucleic Acids and Molecular Biology, 17, Gross, H. J ed., Springer-Verlag, Heidelberg
9. Paddison, P. J., Caudy, A. A., Bernstein, E., Hannon, G. J., and Conklin, D.S. (2002) Short hairpin RNAs (shRNAs) induce sequencespecific silencing in mammalian cells. Genes. Dev., 16, 948-958.
10. Siolas, D., Lerner, C., Burchard, J., Ge, W., Linsley, P. S. et al. (2005) Synthetic shRNAs as potent RNAi triggers. Nature Biotechnol., 23, 227-231.
11. Suzuki, H., Ando, T., Umekage, S., Tanaka, T., and Kikuchi, Y. (2010) Extracellular production of an RNA aptamer by ribonuclease-free marine bacteria harboring engineered plasmids: Proposal for industrial RNA drug production. Appl. Environ. Microbiol., 76, 786-793.
12. Suzuki, H., Daimon, M., Awano, T., Umekage, S., Tanaka, T., and Kikuchi, Y. (2009a) Characterization of extracellular DNA production and flocculation of the marine photosynthetic bacterium Rhodovulum sulfidophilum. Appl. Microbiol. Biotechnol., 84, 349-356.
13. Suzuki, H., Umekage, S., Tanaka, T., and Kikuchi, Y. (2009b) Extracellular tRNAs of the marine photosynthetic bacterium Rhodovulum sulfidophilum are not aminoacylated. Biosci. Biotechnol. Biochem., 73, 425-427.
14. Suzuki, H., Umekage, S., Tanaka, T., and Kikuchi, Y. (2011) Artificial RNA aptamer production by the marine bacterium Rhodovulum sulfidophilum: Improvement of the aptamer yield using a mutated transcriptional promoter. J. Biosci. Bioeng., 112, 458-461.
15. Wilson, K. S. and von Hippel, P. H. (1995) Transcription termination at intrinsic terminators: The role of the RNA hairpin terminators. Proc. Natl. Acad. Sci. USA, 92, 8793-8797.
16. Zhu, B., Cai, G., Hall, E., and Freeman, G. (2007) In-FusionTM assembly: Seamless engineering of multidomain fusion proteins, modular vectors, and mutations. BioTechniques, 43, 354-359