Artificial RNA aptamer production by the marine bacterium Rhodovulum sulfidophilum: Improvement of the aptamer yield using a mutated transcriptional promoter

Journal of Bioscience and Bioengineering

Volumn 112, Issue 5, 2011, Pages 458-461

  Suzuki, Hiromichi ^a   Umekage, So ^a   Tanaka, Terumichi ^a   Kikuchi, Yo ^a  

^a TOYOHASHI UNIVERSITY OF TECHNOLOGY   (Japan)

Author keywords

Functional RNA; Phototrophic bacterium; Promoter; RNA aptamer; Transcription

Indexed keywords

APTAMERS; ARTIFICIAL RNA; CULTURE MEDIUM; ECONOMICAL PRODUCTION; EXTRACELLULAR; MARINE BACTERIUM; PHOTOTROPHIC BACTERIUM; PROMOTER; RNA APTAMER; SMALL RNA; STREPTAVIDIN; THERAPEUTIC AGENTS; VECTOR PLASMIDS;

BACTERIA; DNA; ENZYME ACTIVITY; GENES; PROTEINS; TRANSCRIPTION;

RNA;

APTAMER; BACTERIAL RNA; FACTOR FOR INVERSION STIMULATION;

ARTICLE; BACTERIUM CULTURE; BIOLOGICAL ACTIVITY; CULTURE MEDIUM; GENE MUTATION; GENETIC TRANSCRIPTION; MARINE BACTERIUM; NONHUMAN; PHOTOTROPHIC BACTERIUM; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN MODIFICATION; QUANTITATIVE ANALYSIS; RECOMBINANT PLASMID; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RHODOVULUM; RHODOVULUM SULFIDOPHILUM; RNA ANALYSIS; RNA SYNTHESIS; TRANSCRIPTION REGULATION;

APTAMERS, NUCLEOTIDE; BASE SEQUENCE; CULTURE MEDIA; MOLECULAR SEQUENCE DATA; MUTATION; PLASMIDS; PROMOTER REGIONS, GENETIC; RHODOVULUM; RIBONUCLEASES; RNA, BACTERIAL; STREPTAVIDIN; TRANSCRIPTION, GENETIC;

BACTERIA (MICROORGANISMS); PHOTOBACTERIA; RHODOVULUM SULFIDOPHILUM;

EID: 81255197381     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2011.07.025     Document Type: Article

References (20)

1. Nellen, W. and Hammann, C. (Eds.): Small RNAs: Analysis and regulatory functions. Gross, H.J. (Ed.), Nucleic acids and molecular biology, vol. 17. Springer-Verlag, Heidelberg, Germany (2005).
2. Que-Gewirth N.S., Sullenger B.A. Gene therapy progress and prospects: RNA aptamers. Gene Ther. 2007, 14:283-291.
3. Breaker R.R. Natural and engineered nucleic acids as tools to explore biology. Nature 2004, 432:838-845.
4. Khan A.U. Ribozyme: a clinical tool. Clin. Chim. Acta 2006, 367:20-27.
5. Milligan J.F., Groebe D.R., Witherell G.W., Uhlenbeck O.C. Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates. Nucleic Acids Res. 1987, 15:8783-8798.
6. Marshall W.S., Kaiser R.J. Recent advances in the high-speed solid phase synthesis of RNA. Curr. Opin. Chem. Biol. 2004, 8:222-229.
7. Suzuki H., Ando T., Umekage S., Tanaka T., Kikuchi Y. Extracellular production of an RNA aptamer by ribonuclease-free marine bacteria harboring engineered plasmids: proposal for industrial RNA drug production. Appl. Environ. Microbiol. 2010, 76:786-793.
8. Srisawat C., Engelke D.R. Streptavidin aptamers: affinity tags for the study of RNAs and ribonucleoproteins. RNA 2001, 7:632-641.
9. Suzuki H., Daimon M., Awano T., Umekage S., Tanaka T., Kikuchi Y. Characterization of extracellular DNA production and flocculation of the marine photosynthetic bacterium Rhodovulum sulfidophilum. Appl. Microbiol. Biotechnol. 2009, 84:349-356.
10. Suzuki H., Umekage S., Tanaka T., Kikuchi Y. Extracellular tRNAs of the marine photosynthetic bacterium Rhodovulum sulfidophilum are not aminoacylated. Biosci. Biotechnol. Biochem. 2009, 73:425-427.
11. Hansen T.A., Veldkamp H. Rhodopseudomonas sulfidophila, nov. spec., a new species of the purple nonsulfur bacteria. Arch. Mikrobiol. 1973, 92:45-48.
12. Hiraishi A., Ueda Y. Intrageneric structure of the genus Rhodobacter: transfer of Rhodobacter sulfidophilus and related marine species to the genus Rhodovulum gen. nov. Int. J. Syst. Bacteriol. 1994, 44:15-23.
13. Nagashima K.V.P., Hiraishi A., Shimada K., Matsuura K. Horizontal transfer of genes coding for the photosynthetic reaction centers of purple bacteria. J. Mol. Evol. 1997, 45:131-136.
14. Lee J.K., Kaplan S. Transcriptional regulation of puc operon expression in Rhodobacter sphaeroides. J. Biol. Chem. 1995, 270:20453-20458.
15. Szpirer C.Y., Faelen M., Couturier M. Mobilization function of the pBHR1 plasmid, a derivative of the broad-host-range plasmid pBBR1. J. Bacteriol. 2001, 183:2101-2110.
16. Fornari C.S., Kaplan S. Genetic transformation of Rhodopseudomonas sphaeroides by plasmid DNA. J. Bacteriol. 1982, 152:89-97.
17. Miller J.H. Experiments in molecular genetics 1972, 352-355. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
18. Bradford M.M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72:248-254.
19. Richard C.L., Tandon A., Kranz R.G. Rhodobacter capsulatus nifA1 promoter: high-GC -10 regions in high-GC bacteria and the basis for their transcription. J. Bacteriol. 2004, 186:740-749.
20. Dryden S.C., Kaplan S. Identification of cis-acting regulatory regions upstream of the rRNA operons of Rhodobacter sphaeroides. J. Bacteriol. 1993, 175:6392-6402.