Isolation of rare recombinants without using selectable markers for one-step seamless BACmutagenesis

Nature Methods

Volumn 11, Issue 9, 2014, Pages 966-970

  Lyozin, George T ^a   Bressloff, Paul C ^b   Kumar, Amit ^c   Kosaka, Yasuhiro ^a   Demarest, Bradley L ^a   Yost, H Joseph ^a   Kuehn, Michael R ^c   Brunelli, Luca ^a  

^a UNIVERSITY OF UTAH SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF UTAH   (United States)

^c NATIONAL CANCER INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL MARKER; PROTEIN NODAL; RECOMBINANT PROTEIN; GENETIC MARKER;

ANIMAL EXPERIMENT; ARTICLE; ARTIFICIAL CHROMOSOME; BACTERIAL ARTIFICIAL CHROMOSOME; CONFOCAL MICROSCOPY; ESCHERICHIA COLI; GENETIC ENGINEERING; INDEL MUTATION; LOXP SITE; MOUSE; MUTAGENESIS; NONHUMAN; P1 PHAGE DERIVED ARTIFICIAL CHROMOSOME; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; ANIMAL; GENETIC MARKER; GENETICS; ISOLATION AND PURIFICATION; PROCEDURES; PROTEIN ENGINEERING; SITE DIRECTED MUTAGENESIS;

ANIMALS; CHROMOSOMES, ARTIFICIAL, BACTERIAL; GENETIC MARKERS; MICE; MUTAGENESIS, SITE-DIRECTED; PROTEIN ENGINEERING; RECOMBINANT PROTEINS;

EID: 84921652226     PISSN: 15487091     EISSN: 15487105     Source Type: Journal    
DOI: 10.1038/nmeth.3030     Document Type: Article

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