Dual labeling with 5-bromo-2'-deoxyuridine and 5-ethynyl-2'-deoxyuridine for estimation of cell migration rate in the small intestinal epithelium

Development Growth and Differentiation

Volumn 57, Issue 1, 2015, Pages 68-73

  Asano, Mami ^a   Yamamoto, Tatsuro ^b   Tsuruta, Takeshi ^a   Nishimura, Naomichi ^b   Sonoyama, Kei ^a  

^a HOKKAIDO UNIVERSITY   (Japan)

^b NAYORO CITY UNIVERSITY   (Japan)

Author keywords

5 bromo 2' deoxyuridine; 5 ehynyl 2' deoxyuridine; Cell migration; Intestine

Indexed keywords

5 ETHYNYL 2' DEOXYURIDINE; BROXURIDINE; THYMIDINE DERIVATIVE; UNCLASSIFIED DRUG; 5-ETHYNYL-2'-DEOXYURIDINE; DEOXYURIDINE;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL LABELING; CELL MIGRATION; CLICK CHEMISTRY; ILEUM; IMMUNOHISTOCHEMISTRY; INTESTINE BRUSH BORDER; JEJUNUM; MALE; NONHUMAN; RAT; SMALL INTESTINE MUCOSA; TISSUE SECTION; ANALOGS AND DERIVATIVES; ANIMAL; CELL MOTION; CYTOLOGY; INTESTINE MUCOSA; METABOLISM; PHYSIOLOGY; PROCEDURES; SMALL INTESTINE; STAINING; WISTAR RAT;

ANIMALIA; RATTUS;

ANIMALS; BROMODEOXYURIDINE; CELL MOVEMENT; DEOXYURIDINE; INTESTINAL MUCOSA; INTESTINE, SMALL; MALE; RATS; RATS, WISTAR; STAINING AND LABELING;

EID: 84921533914     PISSN: 00121592     EISSN: 1440169X     Source Type: Journal    
DOI: 10.1111/dgd.12191     Document Type: Article

References (14)

1. Buck, S. B., Bradford, J., Gee, K. R., Agnew, B. J., Clarke, S. T. & Salic, A. 2008. Detection of S-phase cell cycle progression using 5-ethynyl-2'-deoxyuridine incorporation with click chemistry, an alternative to using 5-bromo-2'-deoxyuridine antibodies. Biotechniques 44, 927-929.
2. Cappella, P., Gasparri, F., Pulici, M. & Moll, J. 2008. A novel method based on click chemistry, which overcomes limitations of cell cycle analysis by classical determination of BrdU incorporation, allowing multiplex antibody staining. Cytometry A. 73, 626-636.
3. van der Flier, L. G. & Clevers, H. 2009. Stem cells, self-renewal, and differentiation in the intestinal epithelium. Annu. Rev. Physiol. 71, 241-260.
4. Hsu, T. L., Hanson, S. R., Kishikawa, K., Wang, S. K., Sawa, M. & Wong, C. H. 2007. Alkynyl sugar analogs for the labeling and visualization of glycoconjugates in cells. Proc. Natl Acad. Sci. USA 104, 2614-2619.
5. Ito, H., Satsukawa, M., Arai, E., Sugiyama, K., Sonoyama, K., Kiriyama, S. & Morita, T. 2009. Soluble fiber viscosity affects both goblet cell number and small intestine mucin secretion in rats. J. Nutr. 139, 1640-1647.
6. Lillie, R. D. & Harold, M. F. 1976. Histopathologic Technic and Practical Histochemistry. McGraw-Hill Book Company, New York, NY.
7. Lloyd, R. V. & Wilson, B. S. 1983. Specific endocrine tissue marker defined by a monoclonal antibody. Science 222, 628-630.
8. Melendez, J., Liu, M., Sampson, L., Akunuru, S., Han, X., Vallance, J., Witte, D., Shroyer, N. & Zheng, Y. 2013. Cdc42 coordinates proliferation, polarity, migration, and differentiation of small intestinal epithelial cells in mice. Gastroenterology 145, 808-819.
9. Reeves, P. G., Nielsen, F. H. & Fahey, G. C. Jr, 1993. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J. Nutr. 123, 1939-1951.
10. Salic, A. & Mitchison, T. J. 2008. A chemical method for fast and sensitive detection of DNA synthesis in vivo. Proc. Natl Acad. Sci. USA 105, 2415-2420.
11. Sawa, M., Hsu, T. L., Itoh, T., Sugiyama, M., Hanson, S. R., Vogt, P. K. & Wong, C. H. 2006. Glycoproteomic probes for fluorescent imaging of fucosylated glycans in vivo. Proc. Natl Acad. Sci. USA 103, 12371-12376.
12. Tornøe, C. W., Christensen, C. & Meldal, M. 2002. Peptidotriazoles on solid phase: [1, 2, 3]-triazoles by regiospecific copper(i)-catalyzed 1, 3-dipolar cycloadditions of terminal alkynes to azides. J. Org. Chem. 67, 3057-3064.
13. Tuttle, A. H., Rankin, M. M., Teta, M., Sartori, D. J., Stein, G. M., Kim, G. J., Virgilio, C., Granger, A., Zhou, D., Long, S. H., Schiffman, A. B. & Kushner, J. A. 2010. Immunofluorescent detection of two thymidine analogues (CldU and IdU) in primary tissue. J. Vis. Exp. 46, e2166.
14. Zeng, C., Pan, F., Jones, L. A., Lim, M. M., Griffin, E. A., Sheline, Y. I., Mintun, M. A., Holtzman, D. M. & Mach, R. H. 2010. Evaluation of 5-ethynyl-2'-deoxyuridine staining as a sensitive and reliable method for studying cell proliferation in the adult nervous system. Brain Res. 1319, 21-32.