Differential expression of cytochrome P450 enzymes from the CYP2C subfamily in the human brain

Drug Metabolism and Disposition

Volumn 43, Issue 3, 2015, Pages 353-357

  Booth Depaz, Iris M ^a   Toselli, Francesca ^a,b   Wilce, Peter A ^a   Gillam, Elizabeth M J ^a,b  

^a UNIVERSITY OF QUEENSLAND   (Australia)

^b UNIVERSITY OF TORONTO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOCHROME P450 2C; CYTOCHROME P450 2C19; CYTOCHROME P450 2C9; ARACHIDONIC ACID; CYTOCHROME P-450 CYP2C SUBFAMILY; CYTOCHROME P450;

ALCOHOLISM; AMYGDALOID NUCLEUS; ARTICLE; BASAL GANGLION; BRAIN; CASE REPORT; CELL BODY; CEREBELLUM; CONTROLLED STUDY; DENDRITE; FRONTAL CORTEX; HIPPOCAMPUS; HUMAN; HUMAN TISSUE; MALE; NERVE CELL; NERVE FIBER; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; ADULT; AMINO ACID SEQUENCE; ENZYMOLOGY; GENE EXPRESSION; GENETICS; LIVER; LIVER MICROSOME; METABOLISM; MIDDLE AGED; MOLECULAR GENETICS;

ADULT; ALCOHOLICS; AMINO ACID SEQUENCE; ARACHIDONIC ACID; BRAIN; CYTOCHROME P-450 ENZYME SYSTEM; GENE EXPRESSION; HUMANS; LIVER; MALE; MICROSOMES, LIVER; MIDDLE AGED; MOLECULAR SEQUENCE DATA;

EID: 84921369434     PISSN: 00909556     EISSN: 1521009X     Source Type: Journal    
DOI: 10.1124/dmd.114.061242     Document Type: Article

References (20)

1. Alkayed NJ, Birks EK, Hudetz AG, Roman RJ, Henderson L, and Harder DR (1996) Inhibition of brain P-450 arachidonic acid epoxygenase decreases baseline cerebral blood flow. Am J Physiol 271:H1541-H1546.
2. Booth Depaz IM, Toselli F, Wilce PA, and Gillam EM (2013) Differential expression of human cytochrome P450 enzymes from the CYP3A subfamily in the brains of alcoholic subjects and drug-free controls. Drug Metab Dispos 41:1187-1194.
3. Cuttle L, Munns AJ, Hogg NA, Scott JR, Hooper WD, Dickinson RG, and Gillam EMJ (2000) Phenytoin metabolism by human cytochrome P450: involvement of P450 3A and 2C forms in secondary metabolism and drug-protein adduct formation. Drug Metab Dispos 28: 945-950.
4. Dauchy S, Dutheil F, Weaver RJ, Chassoux F, Daumas-Duport C, Couraud PO, Scherrmann JM, De Waziers I, and Declèves X (2008) ABC transporters, cytochromes P450 and their main transcription factors: expression at the human blood-brain barrier. J Neurochem 107 : 1518-1528.
5. Dauchy S, Miller F, Couraud P-O, Weaver RJ, Weksler B, Romero I-A, Scherrmann J-M, De Waziers I, and Decleves X (2009) Expression and transcriptional regulation of ABC transporters and cytochromes P450 in hCMEC/D3 human cerebral microvascular endothelial cells. Biochem Pharmacol 77:897-909.
6. Dutheil F, Dauchy S, Diry M, Sazdovitch V, Cloarec O, Mellottée L, Bièche I, Ingelman-Sundberg M, Flinois JP, and de Waziers I, et al. (2009) Xenobiotic-metabolizing enzymes and transporters in the normal human brain: regional and cellular mapping as a basis for putative roles in cerebral function. Drug Metab Dispos 37:1528-1538.
7. Gervasini G, Carrillo JA, and Benitez J (2004) Potential role of cerebral cytochrome P450 in clinical pharmacokinetics: modulation by endogenous compounds. Clin Pharmacokinet 43: 693-706.
8. Guengerich FP (2005) Human cytochrome P450 enzymes, in Cytochrome P450 (Ortiz de Montellano PR ed) pp 377-530, Kluwer Academic/Plenum Publishing, New York.
9. Hesse LM, He P, Krishnaswamy S, Hao Q, Hogan K, von Moltke LL, Greenblatt DJ, and Court MH (2004) Pharmacogenetic determinants of interindividual variability in bupropion hydroxylation by cytochrome P450 2B6 in human liver microsomes. Pharmacogenetics 14: 225-238.
10. Hillig T, Krustrup P, Fleming I, Osada T, Saltin B, and Hellsten Y (2003) Cytochrome P450 2C9 plays an important role in the regulation of exercise-induced skeletal muscle blood flow and oxygen uptake in humans. J Physiol 546:307-314.
11. Iliff JJ, Close LN, Selden NR, and Alkayed NJ (2007) A novel role for P450 eicosanoids in the neurogenic control of cerebral blood flow in the rat. Exp Physiol 92:653-658.
12. Klose TS, Blaisdell JA, and Goldstein JA (1999) Gene structure of CYP2C8 and extrahepatic distribution of the human CYP2Cs. J Biochem Mol Toxicol 13:289-295.
13. Knüpfer H, Knüpfer MM, Hotfilder M, and Preiss R (1999) P450-expression in brain tumors. Oncol Res 11:523-528.
14. Löfgren S, Baldwin RM, Hiratsuka M, Lindqvist A, Carlberg A, Sim SC, Schülke M, Snait M, Edenro A, and Fransson-Steen R, et al. (2008) Generation of mice transgenic for human CYP2C18 and CYP2C19: characterization of the sexually dimorphic gene and enzyme expression. Drug Metab Dispos 36:955-962.
15. Luo G, Zeldin DC, Blaisdell JA, Hodgson E, and Goldstein JA (1998) Cloning and expression of murine CYP2Cs and their ability to metabolize arachidonic acid. Arch Biochem Biophys 357: 45-57.
16. McFadyen MC, Melvin WT, and Murray GI (1998) Regional distribution of individual forms of cytochrome P450 mRNA in normal adult human brain. Biochem Pharmacol 55: 825-830.
17. Ohtsuki S, Schaefer O, Kawakami H, Inoue T, Liehner S, Saito A, Ishiguro N, Kishimoto W, Ludwig-Schwellinger E, and Ebner T, et al. (2012) Simultaneous absolute protein quantification of transporters, cytochromes P450, and UDP-glucuronosyltransferases as a novel approach for the characterization of individual human liver: comparison with mRNA levels and activities. Drug Metab Dispos 40:83-92.
18. Riedl AG, Watts PM, Douek DC, Edwards RJ, Boobis AR, Rose S, and Jenner P (2000) Expression and distribution of CYP2C enzymes in rat basal ganglia. Synapse 38: 392-402.
19. Rifkind AB, Lee C, Chang TKH, and Waxman DJ (1995) Arachidonic acid metabolism by human cytochrome P450s 2C8, 2C9, 2E1, and 1A2: regioselective oxygenation and evidence for a role for CYP2C enzymes in arachidonic acid epoxygenation in human liver microsomes. Arch Biochem Biophys 320:380-389.
20. Shukla A, Gillam EM, Mitchell DJ, and Bernhardt PV (2005) Direct electrochemistry of enzymes from the cytochrome P450 2C family. Electrochem Commun 7:437-442.