Inhibited biofilm formation and improved antibacterial activity of a novel nanoemulsion against cariogenic Streptococcus mutans in vitro and in vivo

International Journal of Nanomedicine

Volumn 10, Issue , 2015, Pages 447-462

  Li, Yun Fei ^a,b   Sun, Hong Wu ^a,b   Gao, Rong ^a,b,c   Liu, Kai Yun ^a,b   Zhang, Hua Qi ^d   Fu, Qi Huan ^a,b   Qing, Sheng Li ^a,b   Guo, Gang ^a,b   Zou, Quan Ming ^a,b  

^a National Engineering Research Center of Immunological Products Department of Microbiology and Biochemical Pharmacy College of Pharmacy   (China)

^b Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology   (China)

^c THIRD MILITARY MEDICAL UNIVERSITY   (China)

^d Chongqing Wanzhou Institute for Food and Drug Control   (China)

Author keywords

Antibacterial; Chlorhexidine acetate; Nanoemulsion; Streptococcus mutans

Indexed keywords

CHLORHEXIDINE ACETATE; MYRISTIC ACID ISOPROPYL ESTER; PHOSPHATE BUFFERED SALINE; POLYSORBATE 80; PROPYLENE GLYCOL; SALIVA SUBSTITUTE; ANTIINFECTIVE AGENT; EMULSION; NANOMATERIAL;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIBACTERIAL ACTIVITY; ARTICLE; BACTERIAL MEMBRANE; BACTERICIDAL ACTIVITY; BIOFILM; CONTROLLED STUDY; DENTAL CARIES; DRUG DOSAGE FORM COMPARISON; DRUG RELEASE; DRUG SOLUBILITY; DRUG SOLUTION; DRUG STABILITY; FEMALE; IN VITRO STUDY; IN VIVO STUDY; MINIMUM BACTERICIDAL CONCENTRATION; MINIMUM INHIBITORY CONCENTRATION; NANOEMULSION; NONHUMAN; PARTICLE SIZE; PH; RAT; STREPTOCOCCUS MUTANS; ZETA POTENTIAL; ANIMAL; CHEMISTRY; DRUG EFFECTS; EMULSION; MICROBIOLOGY; PHARMACOLOGY; SPRAGUE DAWLEY RAT; THERAPEUTIC USE;

RATTUS; STREPTOCOCCUS MUTANS;

ANIMALS; ANTI-BACTERIAL AGENTS; BIOFILMS; DENTAL CARIES; EMULSIONS; NANOSTRUCTURES; RATS; RATS, SPRAGUE-DAWLEY; STREPTOCOCCUS MUTANS;

EID: 84921286770     PISSN: 11769114     EISSN: 11782013     Source Type: Journal    
DOI: 10.2147/IJN.S72920     Document Type: Article

References (26)

1. Zander A, Sivaneswaran S, Skinner J, Byun R, Jalaludin B. Risk factors for dental caries in small rural and regional Australian communities. Rural Remote Health. 2013;13(3):2492.
2. Shakeri R, Malekzadeh R, Etemadi A, et al. Association of tooth loss and oral hygiene with risk of gastric adenocarcinoma. Cancer Prev Res (Phila). 2013;6(5):477–482.
3. Prasai Dixit L, Shakya A, Shrestha M, Shrestha A. Dental caries preva­lence, oral health knowledge and practice among indigenous Chepang school children of Nepal. BMC Oral Health. 2013;13(20):5.
4. Liu L, Zhang Y, Wu W, Cheng M, Li Y, Cheng R. prevalence and correlates of dental caries in an elderly population in northeast China. PloS One. 2013;8(11):e78723.
5. Wu L, Chang R, Mu Y, et al. Association between obesity and dental caries in Chinese children. Caries Res. 2013;47(2):171–176.
6. Bagramian RA, Garcia-Godoy F, Volpe AR. The global increase in den­tal caries. A pending public health crisis. Am J Dent. 2009;22(1):3–8.
7. Tong Z, Tao R, Jiang W, et al. In vitro study of the properties of Strep­tococcus mutans in starvation conditions. Arch Oral Biol. 2011;56(11):1306–1311.
8. Karthikeyan R, Amaechi BT, Rawls HR, Lee VA. Antimicrobial activity of nanoemulsion on cariogenic Streptococcus mutans. Arch Oral Biol. 2011;56(5):437–445.
9. Autio-Gold J. The role of chlorhexidine in caries prevention. Oper Dent. 2008;33(6):710–716.
10. Sajjan PG, Nagesh L, Sajjanar M, Reddy SK, Venktesh UG. Compara­tive evaluation of chlorhexidine varnish and fluoride varnish on plaque Streptococcus mutans count – an in vivo study. Intl Jf Dent Hyg. 2013;11(3):191–197.
11. Riggs PD, Braden M, Patel M. Chlorhexidine release from room tem­perature polymerising methacrylate systems. Biomaterials. 2000;21(4):345–351.
12. Musa SH, Basri M, Masoumi HR, et al. Formulation optimization of palm kernel oil esters nanoemulsion-loaded with chloramphenicol suitable for meningitis treatment. Colloids Surf B Biointerfaces. 2013;112:113–119.
13. Sun H, Liu K, Liu W, et al. Development and characterization of a novel nanoemulsion drug-delivery system for potential application in oral delivery of protein drugs. Int J Nanomed. 2012;7:5529–5543.
14. Zhao L, Wei Y, Huang Y, He B, Zhou Y, Fu J. Nanoemulsion improves the oral bioavailability of baicalin in rats: in vitro and in vivo evaluation. Int J Nanomedicine. 2013;8:3769–3779.
15. Gil FJ, Sanchez LA, Espias A, Planell JA. In vitro corrosion behaviour and metallic ion release of different prosthodontic alloys. Int Dent J. 1999;49(6):361–367.
16. Fu D, Pei D, Huang C, Liu Y, Du X, Sun H. Effect of desensitising paste containing 8% arginine and calcium carbonate on biofilm formation of Streptococcus mutans in vitro. J Dent. 2013;41(7):619–627.
17. Keyes PH. Dental caries in the molar teeth of rats. I. Distribution of lesions induced by high-carbohydrate low-fat diets. J Dent Res. 1958;37(6):1077–1087.
18. Keyes PH. Dental caries in the molar teeth of rats. II. A method for diagnosing and scoring several types of lesions simultaneously. J Dent Res. 1958;37(6):1088–1099.
19. Krzysciak W, Jurczak A, Koscielniak D, Bystrowska B, Skalniak A. The virulence of Streptococcus mutans and the ability to form biofilms. Eur J Clin Microbiol Infect Dis. 2013;33(4):499–515.
20. Arslan S, Silici S, Perçin D, Koç AN, Er O. Antimicrobial activity of poplar propolis on mutans streptococci and caries development in rats. Turk J Biol. 2012;(36):65–73.
21. Hwang YY, Ramalingam K, Bienek DR, Lee V, You T, Alvarez R. Antimicrobial activity of nanoemulsion in combination with cetylpyri­dinium chloride in multidrug-resistant Acinetobacter baumannii. Antimicrob Agents Chemother. 2013;57(8):3568–3575.
22. Chepurnov AA, Bakulina LF, Dadaeva AA, Ustinova EN, Chepurnova TS, Baker JR Jr. Inactivation of Ebola virus with a surfactant nanoemulsion. Acta Trop. 2003;87(3):315–320.
23. Hamouda T, Hayes MM, Cao Z, et al. A novel surfactant nanoemul­sion with broad-spectrum sporicidal activity against Bacillus species. J Infect Dis. 1999;180(6):1939–1949.
24. Van Oosten B, Marquardt D, Komljenovic I, Bradshaw JP, Sternin E, Harroun TA. Small molecule interaction with lipid bilayers: a molecular dynamics study of chlorhexidine. J Mol Graph Model. 2014;48:96–104.
25. Song JH, Yang TC, Chang KW, Han SK, Yi HK, Jeon JG. In vitro anti-cariogenic activity of dichloromethane fraction from Rheum undulatum L. root. Arch Pharm Res. 2006;29(6):490–496.
26. Ban SH, Kwon YR, Pandit S, Lee YS, Yi HK, Jeon JG. Effects of a bio-assay guided fraction from Polygonum cuspidatum root on the viability, acid production and glucosyltranferase of mutans streptococci. Fitoterapia. 2010;81(1):30–34.