Next generation digital PCR measurement of hepatitis B virus copy number in formalin-fixed paraffin-embedded hepatocellular carcinoma tissue

Clinical Chemistry

Volumn 61, Issue 1, 2015, Pages 290-296

  Huang, Jing Tao ^a   Liu, Ying Juan ^a   Wang, Jin ^b   Xu, Zhi Gao ^a   Yang, Ying ^a   Shen, Fan ^a   Liu, Xing Hui ^c   Zhou, Xin ^a   Liu, Song Mei ^a  

^a ZHONGNAN HOSPITAL OF WUHAN UNIVERSITY   (China)

^b UNIVERSITY OF TEXAS MD ANDERSON CANCER CENTER   (United States)

^c SECOND MILITARY MEDICAL UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

CHOLINESTERASE; DNA; DOUBLE STRANDED DNA; HEPATITIS B SURFACE ANTIGEN; SINGLE STRANDED DNA; CIRCULAR DNA; PARAFFIN; VIRUS DNA;

ADULT; ARTICLE; ASSESSMENT OF HUMANS; BARCELONA CLINIC LIVER CANCER CLASSIFICATION; CANCER STAGING; CHILD PUGH SCORE; CHOLINESTERASE BLOOD LEVEL; CLINICAL FEATURE; CONTROLLED STUDY; DNA TEMPLATE; DROPLET DIGITAL POLYMERASE CHAIN REACTION; FALSE POSITIVE RESULT; FEMALE; GENE DOSAGE; HEPATITIS B; HUMAN; HUMAN TISSUE; LIVER CELL; LIVER CELL CARCINOMA; LYMPH NODE METASTASIS; MAJOR CLINICAL STUDY; MALE; POLYMERASE CHAIN REACTION; SEROLOGY; VIRUS LOAD; BLOOD; GENETICS; HEPATITIS B VIRUS; ISOLATION AND PURIFICATION; LIVER TUMOR; MIDDLE AGED; PATHOLOGY; PROCEDURES; VIROLOGY;

HEPATITIS B VIRUS;

CARCINOMA, HEPATOCELLULAR; DNA, CIRCULAR; DNA, VIRAL; FEMALE; GENE DOSAGE; HEPATITIS B; HEPATITIS B SURFACE ANTIGENS; HEPATITIS B VIRUS; HUMANS; LIVER NEOPLASMS; MALE; MIDDLE AGED; NEOPLASM STAGING; PARAFFIN EMBEDDING; POLYMERASE CHAIN REACTION;

EID: 84920528661     PISSN: 00099147     EISSN: 15308561     Source Type: Journal    
DOI: 10.1373/clinchem.2014.230227     Document Type: Article

References (31)

1. Ferlay J, Shin HR, Bray F, Forman D, Mathers C, Parkin DM. Estimates of worldwide burden of cancer in 2008: Globocan 2008. Int J Cancer 2010;127:2893-917.
2. Wang J, Chan JY, Fong CC, Tzang CH, Fung KP, Yang M. Transcriptional analysis of doxorubicin-induced cytotoxicity and resistance in human hepatocellular carcinoma cell lines. Liver Int 2009;29:1338-47.
3. Forner A, Llovet JM, Bruix J. Hepatocellular carcinoma. Lancet 2012;379:1245-55.
4. Chen CJ, Yang HI, Su J, Jen CL, You SL, Lu SN, et al. Risk of hepatocellular carcinoma across a biological gradient of serum hepatitis B virus DNA level JAMA 2006;295: 65-73.
5. El-Serag HB. Hepatocellular carcinoma. N Engl J Med 2011;365:1118-27.
6. Sherman M. Hepatocellular carcinoma: epidemiology, surveillance, and diagnosis. Semin Liver Dis 2010;30: 3-16.
7. Lucifora J, Xia Y, Reisinger F, Zhang K, Stadler D, Cheng X, et al. Specific and nonhepatotoxic degradation of nuclear hepatitis B virus cccDNA. Science 2014;343: 1221-8.
8. European Association for the Study of the Liver. EASL clinical practice guidelines: management of chronic hepatitis B virus infection. J Hepatol 2012;57:167-85.
9. Chen JD, Yang HI, Iloeje UH, You SL, Lu SN, Wang LY, et al. Carriers of inactive hepatitis B virus are still at risk for hepatocellular carcinoma and liver-related death. Gastroenterology 2010;138:1747-U38.
10. Hino O, Kitagawa T, Sugano H. Relationship between serum and histochemical markers for hepatitis-B virus and rate of viral integration in hepatocellular carcinomas in Japan. Int J Cancer 1985;35:5-10.
11. Ide T, Kumashiro R, Koga Y, Tanaka E, Hino T, Hisamochi A, et al. A real-time quantitative polymerase chain reaction method for hepatitis B virus in patients with chronic hepatitis B treated with lamivudine. Am J Gastroenterol 2003;98:2048-51.
12. Belgrader P, Tanner SC, Regan JF, Koehler R, Hindson BJ, Brown AS. Droplet digital PCR measurement of Her2 copy number alteration in formalin-fixed paraffinembedded breast carcinoma tissue. Clin Chem 2013; 59:991-4.
13. Hindson BJ, Ness KD, Masquelier DA, Belgrader P, Heredia NJ, Makarewicz AJ, et al. High-throughput droplet digital PCR system for absolute quantitation of DNA copy number. Anal Chem 2011;83:8604-10.
14. Morisset D, Stebih D, Milavec M, Gruden K, Zel J. Quantitative analysis of food and feed samples with droplet digital PCR. PLoS One 2013;8.
15. Miotke L, Lau BT, Rumma RT, Ji HP. High sensitivity detection and quantitation of DNA copy number and single nucleotide variants with single color droplet digital PCR. Anal Chem 2014;86:2618-24.
16. Beck J, Bierau S, Balzer S, Andag R, Kanzow P, Schmitz J, et al. Digital droplet PCR for rapid quantification of donor DNA in the circulation of transplant recipients as a potential universal biomarker of graft injury. Clin Chem 2013;59:1732-41.
17. Hindson CM, Chevillet JR, Briggs HA, Gallichotte EN, Ruf IK, Hindson BJ, et al. Absolute quantification by droplet digital PCR versus analog real-time PCR. Nat Methods 2013;10:1003-5.
18. Heredia NJ, Belgrader P, Wang SL, Koehler R, Regan J, Cosman AM, et al. Droplet digital™ PCR quantitation of Her2 expression in FFPE breast cancer samples. Methods 2013;59:S20-S3.
19. Hayden RT, Gu Z, Ingersoll J, Abdul-Ali D, Shi L, Pounds S, Caliendo AM. Comparison of droplet digital PCR to real-time PCR for quantitative detection of cytomegalovirus. J Clin Microbiol 2013;51:540-6.
20. Whale AS, Huggett JF, Cowen S, Speirs V, Shaw J, Ellison S, et al. Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation. Nucleic Acids Res 2012;40.
21. Kiselinova M, Pasternak AO, De Spiegelaere W, Vogelaers D, Berkhout B, Vandekerckhove L. Comparison of droplet digital PCR and seminested real-time PCR for quantification of cell-associated HIV-1 RNA. PLoS One 2014;9:e85999.
22. White RA 3rd, Quake SR, Curr K. Digital PCR provides absolute quantitation of viral load for an occult RNA virus. J Virol Methods 2012;179:45-50.
23. Li W, Zhao J, Zou Z, Liu Y, Li B, Sun Y, et al. Analysis of hepatitis B virus intrahepatic covalently closed circular DNA and serum viral markers in treatment-naive patients with acute and chronic HBV infection. PLoS One 2014;9:e89046.
24. Qi J, Wang J, Katayama H, Sen S, Liu SM. Circulating microRNAs (cmiRNAs) as novel potential biomarkers for hepatocellular carcinoma. Neoplasma 2013;60:135-42.
25. Chen ML, Shen F, Huang W, Qi JH, Wang YS, Feng YQ, et al. Quantification of 5-methylcytosine and 5-hydroxymethylcytosine in genomic DNA from hepatocellular carcinoma tissues by capillary hydrophilicinteraction liquid chromatography/quadrupole TOF mass spectrometry. Clin Chem 2013;59:824-32.
26. Li XJ, Zhang JB, Yang ZW, Kang JT, Jiang SZ, Zhang T, et al. The function of targeted host genes determines the oncogenicity of HBV integration in hepatocellular carcinoma. J Hepatol 2014;60:975-84.
27. Sung WK, Zheng HC, Li SY, Chen RH, Liu X, Li YR, et al. Genome-wide survey of recurrent HBV integration in hepatocellular carcinoma. Nat Genet 2012;44:765-U188.
28. Jiang SZ, Yang ZW, Li WJ, Li XJ, Wang YF, Zhang JB, et al. Re-evaluation of the carcinogenic significance of hepatitis B virus integration in hepatocarcinogenesis. PLoS One 2012;7.
29. Neuveut C, Wei Y, Buendia MA. Mechanisms of HBVrelated hepatocarcinogenesis. J Hepatol 2010;52: 594-604.
30. Donadon M, Cimino M, Procopio F, Morenghi E, Montorsi M, Torzilli G. Potential role of cholinesterases to predict short-term outcome after hepatic resection for hepatocellular carcinoma. Updates Surg 2013;65: 11-8.
31. Ogunkeye OO, Roluga AI. Serum cholinesterase activity helps to distinguish between liver disease and nonliver disease aberration in liver function tests. Pathophysiology 2006;13:91-3.