Structural basis of a phototropin light switch

Science

Volumn 301, Issue 5639, 2003, Pages 1541-1544

  Harper, Shannon M ^a   Neil, Lori C ^a   Gardner, Kevin H ^a  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYMES; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PROTEINS; SOLUTIONS;

PHOTOTROPINS;

HORMONES;

FLAVINE MONONUCLEOTIDE; PHOSPHOTRANSFERASE; PHOTOTROPIN; UNCLASSIFIED DRUG; VEGETABLE PROTEIN;

LIGHT; PLANT; PROTEIN; RESPONSE ANALYSIS;

ALPHA HELIX; ARTICLE; CONTROLLED STUDY; LIGHT; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PHOTOTROPISM; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN INTERACTION; PROTEIN STRUCTURE; SIGNAL TRANSDUCTION; STRUCTURE ANALYSIS;

AMINO ACID SEQUENCE; AVENA SATIVA; DARKNESS; DROSOPHILA PROTEINS; EYE PROTEINS; FLAVOPROTEINS; LIGHT; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PHOTORECEPTORS, INVERTEBRATE; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; RECEPTORS, G-PROTEIN-COUPLED; SIGNAL TRANSDUCTION;

EID: 0141707094     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1086810     Document Type: Article

References (31)

1. B. L. Taylor, I. B. Zhulin, Microbiol. Mol. Biol. Rev. 63, 479 (1999).
2. E. Huala et al., Science 278, 2120 (1997).
3. S. Crosson, K. Moffat, Proc. Natl. Acad. Sci. U.S.A. 98, 2995 (2001).
4. K. Sakamoto, W. R. Briggs, Plant Cell 14, 1723 (2002).
5. W. R. Briggs, J. H. Christie, Trends Plant Sci. 7, 204 (2002).
6. J. M. Christie, T. E. Swartz, R. A. Bogomolni, W. R. Briggs, Plant J. 32, 205 (2002).
7. M. Salomon, J. M. Christie, E. Knieb, U. Lempert, W. R. Briggs, Biochemistry 39, 9401 (2000).
8. T. E. Swartz et al., J. Biol. Chem. 276, 36493 (2001).
9. S. Crosson, S. Rajagopal, K. Moffat, Biochemistry 42, 2 (2003).
10. R. Fedorov et al., Biophys. J. 84, 2474 (2003).
11. M. Salomon et al., Proc. Natl. Acad. Sci. U.S.A. 98, 12357 (2001).
12. T. E. Swartz, P. J. Wenzel, S. B. Corchnoy, W. R. Briggs, R. A. Bogomolni, Biochemistry 41, 7183 (2002).
13. S. B. Corchnoy et al., J. Biol. Chem. 278, 724 (2003).
14. Materials and methods are available as supporting material on Science Online.
15. J. Cavanagh, W. J. Fairbrother, A. G. Palmer, N. J. Skelton, Protein NMR Spectroscopy: Principles and Practice (Academic Press, San Diego, CA, 1996).
16. B. T. Farmer et al., Nature Struct. Biol. 3, 995 (1996).
17. S. M. Harper, L. C. Neil, K. H. Gardner, unpublished data.
18. Y. Bai, T. R. Sosnick, L. Mayne, S. W. Englander, Science 269, 192 (1995).
19. T. E. Creighton, Proteins: Structure and Molecular Properties (Freeman, New York, 1984).
20. M. A. Gilles-Gonzalez, G. Gonzalez, J. Biol. Chem. 268, 16293 (1993).
21. E. K. Monson, G. S. Ditta, D. R. Helinski, J. Biol. Chem. 270, 5243 (1995).
22. J. Rutter, C. Michnoff, S. M. Harper, K. H. Gardner, S. L. McKnight, Proc. Natl. Acad. Sci. U.S.A. 98, 8991 (2001).
23. C. A. Amezcua, S. M. Harper, J. Rutter, K. H. Gardner, Structure 10, 1349 (2002).
24. K. J. Hettingwerf, J. Hendriks, T. Gensch, J. Phys. Chem. A 107, 1082 (2003).
25. P. Düx et al., Biochemistry 37, 12689 (1998).
26. G. Rubinstenn et al., Nature Struct. Biol. 5, 568 (1998).
27. W. D. Hoff et al., Biochemistry 38, 1009 (1999).
28. D. S. Wishart, B. D. Sykes, J. Biomol. NMR 4, 171 (1994).
29. G. Comilescu, F. Delaglio, A. Bax, J. Biomol. NMR 13, 289 (1999).
30. G. Rubinstenn et al., J. Magn. Reson. 137, 443 (1999).
31. We thank P. J. Hore and I. Day for their assistance with collecting preliminary spectra of photoactivated LOV2 and discussions of equipment design; J. Christie, W. Briggs, and members of the Briggs research group for discussions and reagents; S. Crosson and K. Moffat for insightful discussions; and M. Rosen, M. A. Gilles-Gonzalez, and Y. Liu for critical readings of this manuscript. Supported by NIH training grant award (GM08297) for S.M.H. and grants from the NIH (CA90601) and the Robert A. Welch Foundation (I-1424) to K.H.G.