EZH2 regulates cofilin activity and colon cancer cell migration by targeting ITGA2 gene

PLoS ONE

Volumn 9, Issue 12, 2014, Pages

  Ferraro, Angelo ^a   Boni, Themis ^a   Pintzas, Alexander ^a  

^a INSTITUTE OF BIOLOGY   (Greece)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA2 INTEGRIN; COFILIN; TRANSCRIPTION FACTOR EZH2; ACTIN DEPOLYMERIZING FACTOR; EZH2 PROTEIN, HUMAN; POLYCOMB REPRESSIVE COMPLEX 2; TUMOR PROTEIN;

ARTICLE; CELL MIGRATION; COLORECTAL CANCER; COLORECTAL CANCER CELL LINE; CONTROLLED STUDY; EPIGENETICS; GENE; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENE SILENCING; GENE TARGETING; ITGALPHA2 GENE; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PHOSPHORYLATION; SIGNAL TRANSDUCTION; TRANSCRIPTION REGULATION; CACO 2 CELL LINE; CELL MOTION; COLONIC NEOPLASMS; GENETICS; HUMAN; METABOLISM; PATHOLOGY;

ACTIN DEPOLYMERIZING FACTORS; CACO-2 CELLS; CELL MOVEMENT; COLONIC NEOPLASMS; HUMANS; INTEGRIN ALPHA2; NEOPLASM PROTEINS; POLYCOMB REPRESSIVE COMPLEX 2;

EID: 84920192280     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0115276     Document Type: Article

References (39)

1. Liotta LA, Kohn EC (2001) The microenvironment of the tumour-host interface. Nature 411, 375-379.
2. Condeelis J, Segall JE (2003) Intravital imaging of cell movement in tumours. Nat Rev Cancer 3, 921-930.
3. Bernstein BW, Bamburg JR (2010) ADF/cofilin: a functional node in cell biology. Trends Cell Biol 20, 187-195.
4. Wang W, Eddy R, Condeelis J (2007) The cofilin pathway in breast cancer invasion and metastasis Nat Rev Cancer 7, 429-440.
5. Yamaguchi H, Wyckoff J, Condeelis J (2005) Cell migration in tumors. Curr Opin Cell Biol. 17, 559-64.
6. Wang W, Mouneimne G, Sidani M, Wyckoff J, Chen X, et al. (2006) The activity status of cofilin is directly related to invasion, intravasation, and metastasis of mammary tumors. J Cell Biol. 173, 395-404.
7. Yang N, Higuchi O, Ohashi K, Nagata K, Wada A, et al. (1998) Cofilin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization. Nature 393, 809-812.
8. Toshima J, Toshima JY, Amano T, Yang N, Narumiya S, et al. (2001) Cofilin phosphorylation by protein kinase testicular protein kinase 1 and its role in integrin-mediated actin reorganization and focal adhesion formation. Mol Biol Cell. 12, 1131-1145.
9. Niwa R, Nagata-Ohashi K, Takeichi M, Mizuno K, Uemura T (2002) Control of actin reorganization by Slingshot, a family of phosphatases that dephosphorylate ADF/cofilin. Cell; 108, 233-246.
10. Gohla A, Birkenfeld J, Bokoch G (2005) Chronophin, a novel HAD-type serine protein phosphatase, regulates cofilin-dependent actin dynamics. Nat Cell Biol. 7, 21-29.
11. Agnew BJ, Minamide LS, Bamburg JR. (1995) Reactivation of phosphorylated actin depolymerizing factor and identification of the regulatory site. J Biol Chem 270, 17582-17587.
12. Mizuno K (2013) Signaling mechanisms and functional roles of cofilin phosphorylation and dephosphorylation. Cell Signal. 25, 457-469.
13. Cao R, Wang L, Wang H, Xia L, Erdjument-Bromage H, et al. (2002) Role of histone H3 lysine 27 methylation in Polycomb-group silencing. Science 298, 1039-1043.
14. Varambally S, Dhanasekaran S, Zhou M, Barrette T, Kumar-Sinha C, et al. (2002) The polycomb group protein EZH2 is involved in progression of prostate cancer. Nature 419, 624-629.
15. Kleer C, Cao Q, Varambally S, Shen R, Ota I, et al. (2003) EZH2 is a marker of aggressive breast cancer and promotes neoplastic transformation of breast epithelial cells. Proc Natl Acad Sci U S A. 100, 11606-11611.
16. Yu J, Yu J, Rhodes D, Tomlins S, Cao X, et al. (2007) A polycomb repression signature in metastatic prostate cancer predicts cancer outcome. Cancer Res. 67, 10657-10663.
17. Ferraro A, Mourtzoukou D, Kosmidou V, Avlonitis S, Kontogeorgos G, et al. (2013) EZH2 is regulated by ERK/AKT and targets integrin alpha2 gene to control Epithelial-Mesenchymal Transition and anoikis in colon cancer cells. Int J Biochem Cell Biol. 45, 243-254.
18. Gonzalez M, Li X, Toy K, DuPrie M, Ventura A, et al. (2009) Downregulation of EZH2 decreases growth of estrogen receptor-negative invasive breast carcinoma and requires BRCA1. Oncogene 28, 843-853.
19. Lu C, Han, Mangala L, Ali-Fehmi R, Newton C, et al. (2010) Regulation of tumor angiogenesis by EZH2. Cancer Cell 18, 185-197.
20. Hynes RO (2002) Integrins: bidirectional, allosteric signaling machines. Cell 110, 673-687.
21. Zutter MM, Santoro SA, Staatz WD, Tsung YL (1995) Re-expression of the alpha 2 beta 1 integrin abrogates the malignant phenotype of breast carcinoma cells. Proc Natl Acad Sci U S A 92, 7411-7415.
22. Ivaska J, Reunanen H, Westermarck J, Koivisto L, Kähäri VM, et al. (1999) Integrin alpha2beta1 mediates isoform-specific activation of p38 and upregulation of collagen gene transcription by a mechanism involving the alpha2 cytoplasmic tail. J Cell Biol 147, 401-416.
23. Ellinger-Ziegelbauer H, Kelly K, Siebenlist U (1999) Cell cycle arrest and reversion of Ras-induced transformation by a conditionally activated form of mitogen-activated protein kinase kinase kinase 3. Mol Cell Biol 19:, 3857-3868.
24. Ramirez NE, Zhang Z, Madamanchi A, Boyd KL, O'Rear LD, et al. (2011) The ab integrin is a metastasis suppressor in mouse models and human cancer. J Clin Invest 121, 226-2237.
25. Shaikhibrahim Z, Lindstrot A, Buettner R, Wernert N (2011) Analysis of laser-microdissected prostate cancer tissues reveals potential tumor markers. Int J Mol Med 28, 605-611.
26. Tan J, Yang X, Zhuang L, Jiang X, Chen W, et al. (2007). Pharmacologic disruption of Polycomb-repressive complex 2-mediated gene repression selectively induces apoptosis in cancer cells. Genes & development 21, 1050-1063.
27. Verma S, Tian X, LaFrance L, Duquenne C, Suarez D, et al. (2013) Identification of potent, selective, cell-active inhibitors of the histone lysine methyltransferase EZH2. ACS Medicinal Chemistry Letters 3, 1091-1096.
28. LaLonde DP, Brown MC, Bouverat BP, Turner CE (2005) Actopaxin interacts with TESK1 to regulate cell spreading on fibronectin. J Biol Chem. 280, 21680-21688.
29. Makrodouli E, Oikonomou E, Koc M, Andera L, Sasazuki T, et al. (2011) BRAF and RAS oncogenes regulate Rho GTPase pathways to induce migration and invasion properties in human colon cancer cells: a comparative study. Mol. Cancer 10, 118-139.
30. Ren G, Baritaki S, Marathe H, Feng J, Park S, et al. (2012) Polycomb protein EZH2 regulates tumor invasion via the transcriptional repression of the metastasis suppressor RKIP in breast and prostate cancer. Cancer Res. 72, 3091-3104.
31. Min J, Zaslavsky A, Fedele G, McLaughlin S, Reczek E, et al. (2010) An oncogene-tumor suppressor cascade drives metastatic prostate cancer by coordinately activating Ras and nuclear factor-kappaB. Nat Med. 16, 286-294.
32. Rao Z, Cai M, Yang G, He L, Mai S, et al. (2010) EZH2 supports ovarian carcinoma cell invasion and/or metastasis via regulation of TGF-beta1 and is a predictor of outcome in ovarian carcinoma patients. Carcinogenesis. 31, 1576-1583.
33. Dawe HR, Minamide LS, Bamburg JR, Cramer LP (2003) ADF/cofilin controls cell polarity during fibroblast migration. Curr Biol. 13, 252-257.
34. van Rheenen J, Condeelis J, Glogauer M (2009) A common cofilin activity cycle in invasive tumor cells and inflammatory cells. Journal of cell science 122, 305-311.
35. Madak-Erdogan Z, Ventrella R, Petry L, Katzenellenbogen B (2014) Novel Roles for ERK5 and Cofilin as Critical Mediators Linking ERalpha-Driven Transcription, Actin Reorganization and Invasiveness in Breast Cancer. Molecular Cancer Research. 12, 714-727.
36. Frantz C, Barreiro G, Dominguez L, Chen X, Eddy R, et al. (2008) Cofilin is a pH sensor for actin free barbed end formation: role of phosphoinositide binding. J Cell Biol. 183, 865-879.
37. Oser M, Yamaguchi H, Mader CC, Bravo-Cordero JJ, Arias M, et al. (2009) Cortactin regulates cofilin and N-WASp activities to control the stages of invadopodium assembly and maturation. J Cell Biol. 186, 571-587.
38. Bernstein BW, Painter WB, Chen H, Minamide LS, Abe H, et al. (2000) Intracellular pH modulation of ADF/cofilin proteins. Cell Motil Cytoskeleton. 47, 319-336.
39. Huttenlocher A, Lakonishok M, Kinder M, Wu S, Truong T, et al. (1998) Integrin and cadherin synergy regulates contact inhibition of migration and motile activity. The Journal of cell biology 141, 515-526.