Melittin radiosensitizes esophageal squamous cell carcinoma with induction of apoptosis in vitro and in vivo

Tumor Biology

Volumn 35, Issue 9, 2014, Pages 8699-8705

  Zhu, Hongcheng ^a   Yang, Xi ^a   Liu, Jia ^a   Ge, Yangyang ^b   Qin, Qin ^a   Lu, Jing ^a   Zhan, Liangliang ^a   Liu, Zheming ^a   Zhang, Hao ^a   Chen, Xiaochen ^a   Zhang, Chi ^a   Xu, Liping ^a   Cheng, Hongyan ^a   Sun, Xinchen ^a  

^a THE FIRST AFFILIATED HOSPITAL OF NANJING MEDICAL UNIVERSITY   (China)

^b Nantong Tumor Hospital   (China)

Author keywords

Apoptosis; Esophageal squamous cell carcinoma; Melittin; Radiosensitivity

Indexed keywords

APOPTOSIS REGULATORY PROTEIN; MELITTIN; PROTEIN BAX; PROTEIN BCL 2; RADIOSENSITIZING AGENT;

ANIMAL EXPERIMENT; ANIMAL MODEL; APOPTOSIS; ARTICLE; CANCER RADIOTHERAPY; CANCER SURVIVAL; CELL ASSAY; CELL COUNTING; CELL PROLIFERATION; CELL SURVIVAL; CLONOGENIC ASSAY; CONTROLLED STUDY; DRUG POTENCY; ESOPHAGEAL SQUAMOUS CELL CARCINOMA; FLOW CYTOMETRY; IN VITRO STUDY; IN VIVO STUDY; MALE; MOUSE; NONHUMAN; PROTEIN EXPRESSION; RADIOSENSITIVITY; RADIOSENSITIZATION; SQUAMOUS CELL CARCINOMA CELL LINE; TUMOR XENOGRAFT; ANIMAL; BAGG ALBINO MOUSE; CARCINOMA, SQUAMOUS CELL; CHEMORADIOTHERAPY; CONFOCAL MICROSCOPY; DOSE RESPONSE; DRUG EFFECTS; DRUG SCREENING; ESOPHAGEAL NEOPLASMS; HUMAN; IMMUNOBLOTTING; METABOLISM; NUDE MOUSE; PATHOLOGY; PROCEDURES; RADIATION RESPONSE; TUMOR CELL LINE;

ANIMALS; APOPTOSIS; BCL-2-ASSOCIATED X PROTEIN; CARCINOMA, SQUAMOUS CELL; CELL LINE, TUMOR; CELL PROLIFERATION; CELL SURVIVAL; CHEMORADIOTHERAPY; DOSE-RESPONSE RELATIONSHIP, DRUG; DOSE-RESPONSE RELATIONSHIP, RADIATION; ESOPHAGEAL NEOPLASMS; FLOW CYTOMETRY; HUMANS; IMMUNOBLOTTING; MALE; MELITTEN; MICE, INBRED BALB C; MICE, NUDE; MICROSCOPY, CONFOCAL; PROTO-ONCOGENE PROTEINS C-BCL-2; RADIATION-SENSITIZING AGENTS; XENOGRAFT MODEL ANTITUMOR ASSAYS;

EID: 84919835224     PISSN: 10104283     EISSN: None     Source Type: Journal    
DOI: 10.1007/s13277-014-2146-z     Document Type: Article

References (26)

1. Jemal A, Bray F, Center MM, et al. Global cancer statistics. CA Cancer J Clin. 2011;61:69–90.
2. Chen W, Zheng R, Zhang S, et al. Annual report on status of cancer in China, 2010. Chin J Cancer Res. 2014;26:48–58.
3. Chen W, He Y, Zheng R, et al. Esophageal cancer incidence and mortality in China, 2009. J Thorac Dis. 2013;5:19–26.
4. Shoji M, Ninomiya I, Makino I, et al. Valproic acid, a histone deacetylase inhibitor, enhances radiosensitivity in esophageal squamous cell carcinoma. Int J Oncol. 2012;40:2140–6.
5. Huang C, Jin H, Qian Y, et al. Hybrid melittin cytolytic Peptide-driven ultrasmall lipid nanoparticles block melanoma growth in vivo. ACS Nano. 2013;7:5791–800.
6. Wooddell CI, Rozema DB, Hossbach M, et al. Hepatocyte-targeted RNAi therapeutics for the treatment of chronic hepatitis B virus infection. Mol Ther. 2013;21:973–85.
7. Liu H, Han Y, Fu H, et al. Construction and expression of sTRAIL-melittin combining enhanced anticancer activity with antibacterial activity in Escherichia coli. Appl Microbiol Biotechnol. 2013;97:877–2884.
8. Park JH, Kim KH, Lee WR, et al. Protective effect of melittin on inflammation and apoptosis in acute liver failure. Apoptosis. 2012;17:61–9.
9. Liu M, Zong J, Liu Z, et al. A novel melittin-MhIL-2 fusion protein inhibits the growth of human ovarian cancer SKOV3 cells in vitro and in vivo tumor growth. Cancer Immunol Immunother. 2013;62:889–95.
10. Rim DS, Altschuld RA, Ganote CE. Effects of calcium depletion and loading on injury during metabolic inhibition of isolated adult rat myocytes. J Mol Cell Cardiol. 1990;22:227–37.
11. Jo M, Park MH, Kollipara PS, et al. Anti-cancer effect of bee venom toxin and melittin in ovarian cancer cells through induction of death receptors and inhibition of JAK2/STAT3 pathway. Toxicol Appl Pharmacol. 2012;258:72–81.
12. Muller UR. Hymenoptera venom proteins and peptides for diagnosis and treatment of venom allergic patients. Inflamm Allergy Drug Targets. 2011;10:420–8.
13. Saugar JM, Rodriguez-Hernandez MJ, de la Torre BG, et al. Activity of cecropin A-melittin hybrid peptides against colistin-resistant clinical strains of Acinetobacter baumannii: molecular basis for the differential mechanisms of action. Antimicrob Agents Chemother. 2006;50:1251–6.
14. Binder M, Fierlbeck G, King T, et al. Individual hymenoptera venom compounds induce upregulation of the basophil activation marker ectonucleotide pyrophosphatase/phosphodiesterase 3 (CD203c) in sensitized patients. Int Arch Allergy Immunol. 2002;129:160–8.
15. Jeong YJ, Choi Y, Shin JM et al. Melittin suppresses EGF-induced cell motility and invasion by inhibiting PI3K/Akt/mTOR signaling pathway in breast cancer cells. Food Chem Toxicol 2014.
16. Gajski G, Garaj-Vrhovac V. Melittin: a lytic peptide with anticancer properties. Environ Toxicol Pharmacol. 2013;36:697–705.
17. Glinka EM. Eukaryotic expression vectors bearing genes encoding cytotoxic proteins for cancer gene therapy. Plasmid. 2012;68:69–85.
18. Park MH, Choi MS, Kwak DH, et al. Anti-cancer effect of bee venom in prostate cancer cells through activation of caspase pathway via inactivation of NF-kappaB. Prostate. 2011;71:801–12.
19. Zhang HP, Takayama K, Su B, et al. Effect of sunitinib combined with ionizing radiation on endothelial cells. J Radiat Res. 2011;52:1–8.
20. Efimova EV, Liang H, Pitroda SP, et al. Radioresistance of Stat1 over-expressing tumour cells is associated with suppressed apoptotic response to cytotoxic agents and increased IL6-IL8 signalling. Int J Radiat Biol. 2009;85:421–31.
21. Wang C, Chen T, Zhang N, et al. Melittin, a major component of bee venom, sensitizes human hepatocellular carcinoma cells to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis by activating CaMKII-TAK1-JNK/p38 and inhibiting IkappaBalpha kinase-NFkappaB. J Biol Chem. 2009;284:3804–13.
22. Kim SJ, Park JH, Kim KH, et al. Melittin inhibits atherosclerosis in LPS/high-fat treated mice through atheroprotective actions. J Atheroscler Thromb. 2011;18:1117–26.
23. Kim SK, Park KY, Yoon WC, et al. Melittin enhances apoptosis through suppression of IL-6/sIL-6R complex-induced NF-kappaB and STAT3 activation and Bcl-2 expression for human fibroblast-like synoviocytes in rheumatoid arthritis. Joint Bone Spine. 2011;78:471–7.
24. Ezekwudo D, Shashidharamurthy R, Devineni D, et al. Inhibition of expression of anti-apoptotic protein Bcl-2 and induction of cell death in radioresistant human prostate adenocarcinoma cell line (PC-3) by methyl jasmonate. Cancer Lett. 2008;270:277–85.
25. Basu A, Haldar S. The relationship between BcI2, Bax and p53: consequences for cell cycle progression and cell death. Mol Hum Reprod. 1998;4:1099–109.
26. Jiang H, Zhao PJ, Su D et al. Paris saponin I induces apoptosis via increasing the Bax/Bcl2 ratio and caspase3 expression in gefitinibresistant nonsmall cell lung cancer in vitro and in vivo. Mol Med Rep 2014.