Refolded scFv antibody fragment against myoglobin shows rapid reaction kinetics

International Journal of Molecular Sciences

Volumn 15, Issue 12, 2014, Pages 23658-23671

  Song, Hyung Nam ^a,b   Jang, Jun Hyuck ^b   Kim, Young Wan ^b   Kim, Dong Hyung ^b   Park, Sung Goo ^a   Lee, Myung Kyu ^a   Paek, Se Hwan ^b   Woo, Eui Jeon ^a,c  

^a Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^b Korea University   (South Korea)

^c University of Science and Technology (UST)   (South Korea)

Author keywords

Acute myocardial infarction; Myoglobin; Premature antibody; Single chain variable fragment (scFv)

Indexed keywords

ARGININE; COMPLEMENTARY DNA; HEMOGLOBIN; IMMUNOGLOBULIN G; MYOGLOBIN; OVOTRANSFERRIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; TROPONIN I;

ARTICLE; BINDING AFFINITY; CHEMICAL REACTION KINETICS; CONTROLLED STUDY; DEGRADATION KINETICS; DISSOCIATION; ENZYME KINETIC ASSAY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; HEART INFARCTION; HUMAN; HUMAN CELL; IMMUNIZATION; MOLECULAR WEIGHT; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DENATURATION; PROTEIN EXPRESSION; PROTEIN ISOLATION; PROTEIN PURIFICATION; PROTEIN REFOLDING; WESTERN BLOTTING; CHEMISTRY; IMMUNOLOGY; KINETICS;

ESCHERICHIA COLI;

HUMANS; KINETICS; MYOGLOBIN; PROTEIN REFOLDING; SINGLE-CHAIN ANTIBODIES;

EID: 84919798035     PISSN: 16616596     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms151223658     Document Type: Article

References (38)

1. Kim, D.H.; Seo, S.M.; Paek, S.H.; Lim, G.S. Premature antibodies with rapid reaction kinetics and their characterization for diagnostic applications. Anal. Biochem. 2012, 420, 54-60.
2. Cho, H.K.; Seo, S.M.; Cho, I.H.; Paek, S.H.; Kim, D.H. Minimum-step immuno-analysis based on continuous recycling of the capture antibody. Analyst 2011, 136, 1374-1379.
3. Paek, S.H.; Cho, I.H.; Seo, S.M.; Kim, D.H. Production of rapidly reversible antibody and its performance characterization as binder for continuous glucose monitoring. Analyst 2011, 136, 4268-4276.
4. Kim, D.H.; Paek, S.H.; Lim, G.S.; Jeon, J.W. Performance characteristics of monoclonal antibodies as recyclable binders to cardiac troponin I. Anal. Biochem. 2012, 431, 11-18.
5. Sagawa, T.; Oda, M.; Ishimura, M.; Furukawa, K.; Azuma, T. Thermodynamic and kinetic aspects of antibody evolution during the immune response to hapten. Mol. Immunol. 2003, 39, 801-808.
6. Boes, M. Role of natural and immune igm antibodies in immune responses. Mol. Immunol. 2000, 37, 1141-1149.
7. Strandh, M.; Andersson, H.S.; Ohlson, S. Weak affinity chromatography. Methods Mol. Biol. 2000, 147, 7-23.
8. Ohlson, S.; Jungar, C.; Strandh, M.; Mandenius, C.F. Continuous weak-affinity immunosensing. Trends Biotechnol. 2000, 18, 49-52.
9. Jungar, C.; Strandh, M.; Ohlson, S.; Mandenius, C.F. Analysis of carbohydrates using liquid chromatography—Surface plasmon resonance immunosensing systems. Anal. Biochem. 2000, 281, 151-158.
10. Paek, S.H.; Cho, I.H.; Kim, D.H.; Jeon, J.W.; Lim, G.S. Label-free, needle-type biosensor for continuous glucose monitoring based on competitive binding. Biosens. Bioelectron. 2013, 40, 38-44.
11. Kagen, L.; Scheidt, S.; Roberts, L.; Porter, A.; Paul, H. Myoglobinemia following acute myocardial infarction. Am. J. Med. 1975, 58, 177-182.
12. Chiu, A.; Chan, W.K.; Cheng, S.H.; Leung, C.K.; Choi, C.H. Troponin-I, myoglobin, and mass concentration of creatine kinase-MB in acute myocardial infarction. QJM 1999, 92, 711-718.
13. Gnedenko, O.V.; Mezentsev, Y.V.; Molnar, A.A.; Lisitsa, A.V.; Ivanov, A.S.; Archakov, A.I. Highly sensitive detection of human cardiac myoglobin using a reverse sandwich immunoassay with a gold nanoparticle-enhanced surface plasmon resonance biosensor. Anal. Chim. Acta 2013, 759, 105-109.
14. Timucin, C.; Gul, O.; Kutuk, O.; Basaga, H. Antibody array-based immunosensor for detecting cardiovascular disease risk markers. J. Immunoass. Immunochem. 2012, 33, 275-290.
15. Henares, T.G.; Mizutani, F.; Hisamoto, H. Current development in microfluidic immunosensing chip. Anal. Chim. Acta 2008, 611, 17-30.
16. Lee, Y.H.; Iijima, M.; Kado, Y.; Mizohata, E.; Inoue, T.; Sugiyama, A.; Doi, H.; Shibasaki, Y.; Kodama, T. Construction and characterization of functional anti-epiregulin humanized monoclonal antibodies. Biochem. Biophys. Res. Commun. 2013, 441, 1011-1017.
17. Dobhal, S.; Chaudhary, V.K.; Singh, A.; Pandey, D.; Kumar, A.; Agrawal, S. Expression of recombinant antibody (single chain antibody fragment) in transgenic plant Nicotiana tabacum cv. Xanthi. Mol. Biol. Rep. 2013, 40, 7027-7037.
18. Kobayashi, N.; Odaka, K.; Uehara, T.; Imanaka-Yoshida, K.; Kato, Y.; Oyama, H.; Tadokoro, H.; Akizawa, H.; Tanada, S.; Hiroe, M, et al. Toward in vivo imaging of heart disease using a radiolabeled single-chain Fv fragment targeting Tenascin-C. Anal. Chem. 2011, 83, 9123-9130.
19. Gilmartin, A.A.; Lamp, B.; Rumenapf, T.; Persson, M.A.; Rey, F.A.; Krey, T. High-level secretion of recombinant monomeric murine and human single-chain Fv antibodies from drosophila S2 cells. Protein Eng. Des. Sel. 2012, 25, 59-66.
20. Vaks, L.; Benhar, I. Production of stabilized scFv antibody fragments in the E. coli bacterial cytoplasm. Methods Mol. Biol. 2014, 1060, 171-184.
21. Onishi, H.; Mizukami, M.; Hanagata, H.; Tokunaga, M.; Arakawa, T.; Miyauchi, A. Efficient production of anti-fluorescein and anti-lysozyme as single-chain anti-body fragments (scFv) by Brevibacillus expression system. Protein Expr. Purif. 2013, 91, 184-191.
22. Huston, J.S.; Levinson, D.; Mudgett-Hunter, M.; Tai, M.S.; Novotny, J.; Margolies, M.N.; Ridge, R.J.; Bruccoleri, R.E.; Haber, E.; Crea, R, et al. Protein engineering of antibody binding sites: Recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in escherichia coli. Proc. Natl. Acad. Sci. USA 1988, 85, 5879-5883.
23. Worn, A.; Pluckthun, A. Stability engineering of antibody single-chain Fv fragments. J. Mol. Biol. 2001, 305, 989-1010.
24. Joosten, V.; Lokman, C.; van den Hondel, C.A.; Punt, P.J. The production of antibody fragments and antibody fusion proteins by yeasts and filamentous fungi. Microb. Cell Fact. 2003, 2, 1.
25. Pluckthun, A. Escherichia coli producing recombinant antibodies. Bioprocess Technol. 1994, 19, 233-252.
26. Gu, Z.; Weidenhaupt, M.; Ivanova, N.; Pavlov, M.; Xu, B.; Su, Z.G.; Janson, J.C. Chromatographic methods for the isolation of, and refolding of proteins from, escherichia coli inclusion bodies. Protein Expr. Purif. 2002, 25, 174-179.
27. Huang, Q.; Chen, L.; Zeng, L.; Wan, L.; Li, S.; Lu, X.; Cheng, J. Evaluation of three in vitro refolding methods for human-derived anti-CTLA4 scFv expressed in E. coli. Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 2006, 23, 388-391. (In Chinese)
28. Song, H.N.; Kim, D.H.; Paek, S.H.; Park, S.G.; Lee, M.K.; Woo, E.J. Purification and characterization of Fab fragments with rapid reaction kinetics against myoglobin. Biosci. Biotech. Biochem. 2014, in press.
29. Umetsu, M.; Tsumoto, K.; Hara, M.; Ashish, K.; Goda, S.; Adschiri, T.; Kumagai, I. How additives influence the refolding of immunoglobulin-folded proteins in a stepwise dialysis system. Spectroscopic evidence for highly efficient refolding of a single-chain Fv fragment. J. Biol. Chem. 2003, 278, 8979-8987.
30. Choi, S.P.; Park, Y.C.; Lee, J.; Sim, S.J.; Chang, H.N. Effects of L-arginine on refolding of lysine-tagged human insulin-like growth factor 1 expressed in Escherichia coli. Bioprocess Biosyst. Eng. 2012, 35, 255-263.
31. Tischer, A.; Lilie, H.; Rudolph, R.; Lange, C. L-Arginine hydrochloride increases the solubility of folded and unfolded recombinant plasminogen activator rPA. Protein Sci. 2010, 19, 1783-1795.
32. Lu, D.; Liu, Z. Dynamic redox environment-intensified disulfide bond shuffling for protein refolding in vitro: Molecular simulation and experimental validation. J. Phys. Chem. B 2008, 112, 15127-15133.
33. St John, R.J.; Carpenter, J.F.; Randolph, T.W. High-pressure refolding of disulfide-cross-linked lysozyme aggregates: Thermodynamics and optimization. Biotechnol. Prog. 2002, 18, 565-571.
34. Liu, J.L.; Zabetakis, D.; Acevedo-Velez, G.; Goldman, E.R.; Anderson, G.P. Comparison of an antibody and its recombinant derivative for the detection of the small molecule explosive 2,4,6-trinitrotoluene. Anal. Chim. Acta 2013, 759, 100-104.
35. Muramatsu, H.; Yoshikawa, K.; Hayashi, T.; Takasu, S.; Kawada, Y.; Uchida, K.; Sato, S.; Takahashi, T.; Saga, S.; Ueda, R. Production and characterization of an active single-chain variable fragment antibody recognizing CD25. Cancer Lett. 2005, 225, 225-236.
36. Rio, D.C.; Ares, M., Jr.; Hannon, G.J.; Nilsen, T.W. Purification of rna using trizol (tri reagent). Cold Spring Harb. Protoc. 2010, 2010, pdb-prot5439.
37. Lilie, H.; Schwarz, E.; Rudolph, R. Advances in refolding of proteins produced in E. coli. Curr. Opin. Biotechnol. 1998, 9, 497-501.
38. Wetlaufer, D.B.; Branca, P.A.; Chen, G.X. The oxidative folding of proteins by disulfide plus thiol does not correlate with redox potential. Protein Eng. 1987, 1, 141-146.